A note on the isolation of salmonellas from environmental samples using three formulations of Rappaport's broth

Fricker, C. R.; Girdwood, R. W. A.

doi:10.1111/j.1365-2672.1985.tb01470.x

Cited by 12 publications

(4 citation statements)

References 14 publications

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“…The modifications of Vassiliadis (1983) to the Rappaport broth consisted of a pre‐enrichment step followed by an enrichment step, and then streaking onto solid medium. The sensitivity and specificity of RV broth was increased significantly but the method is laborious, time‐consuming and costly ( Fricker and Girdwood 1985). The same medium was modified by the substitution of the enrichment and streaking step with a semi‐solid agar ( De Smedt et al .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of two assays, MSRV and RV, for the isolation of Salmonella spp. from wastewater samples and broiler chickens

Ζdragas

Tsakos²,

Mavrogeni

2000

Lett Appl Microbiol

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A . Z D R A G A S , P . T S A K O S A N D P . M A V R O G E N I . 2000.The Rappaport Vassiliadis (RV) and the Modi®ed Semi-solid Rappaport Vassiliadis (MSRV) methods were evaluated in 130 municipal wastewater samples and 30¯ocks of broilers. Analysis of the results showed that 71 Salmonella serotypes were isolated in wastewater samples. The positivity of the MSRV method was 33% and of the RV method, 45%. The sensitivity was 95% and 83% with the MSRV and RV, respectively. The concordance between the two methods was 89% (k 0785). One hundred serotypes were isolated from broiler internal organs and 50 from intestinal samples. For internal organs, the positivity of MSRV was 56% and of RV, 45%. For intestinal samples, the respective percentages were 28 and 22. For internal organs, the sensitivity was 100% with MSRV and 81% with RV, whereas for intestinal samples, the sensitivity was 100% with MSRV and 80% with RV. The speci®city was 100% in all cases. The concordance between the two methods was 89% (k 0790) for internal organs and 94% (k 0851) for intestinal samples.

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Section: Introductionmentioning

confidence: 99%

Evaluation of two assays, MSRV and RV, for the isolation of Salmonella spp. from wastewater samples and broiler chickens

Ζdragas

Tsakos²,

Mavrogeni

2000

Lett Appl Microbiol

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“…Rappaport et al (1956) designed a medium that contained magnesium chloride (4% w/v) and a new inhibitor, malachite green (0.012% w/v). The Rappaport culture medium has been modified and evaluated by Vassiliadis and co-workers (1974Vassiliadis and co-workers ( , 1979Vassiliadis and co-workers ( , 1981aVassiliadis and co-workers ( ,b, 1983Vassiliadis and co-workers ( , 1985, Papadakis et al (1976), Harvey andPrice (1979, 1983), Kalapothaki et al (1980,1983,1986), Renaud (1983), Fricker (1984), Fricker and Girwood (1985), Watson (1985), De Smedt et al (1986), De Smedt andBolderdijk (1987), Rhodes andQuesnel (1986), Beckers et al (1987), Van Schothorst et al (1987).…”

Section: Introductionmentioning

confidence: 99%

Selective action of inhibitors used in different culture media on the competitive microflora of Salmonella

Arroyo¹,

Arroyo

1995

Journal of Applied Bacteriology

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The action of 12 inhibitors employed in the culture media used to detect the presence of Salmonella in food on 24 bacterial strains including contaminating Gram-positive bacteria common in water and food, Gram-negative bacteria, especially Enterobacteriaceae and Pseudomonadaceae, which are components of the competitive microflora, and six Salmonella serotypes was tested. Two liquid culture media (AR 5 and AE 1) were used. Series of tubes containing increasing concentrations of each inhibitor were inoculated with the test strains and incubated at 37 degrees C until growth was verified spectrophotometrically (24-48 h). The results showed that the inhibitors were effective against the Gram-positive contaminating microflora. They did not preferentially inhibit the competitive microflora of Salmonella, chiefly Enterobacteriaceae, and were ineffective against the Pseudomonas strains, which can tolerate concentrations higher than those customarily employed in culture media.

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“…This medium has subsequently become known as Rappaport-Vassiliadis (RV) medium (Papadakis & Efstratiou, 1980). Since 1976 the results of many studies of the relative efficiency of RV medium have been published, and whilst some workers maintain that the R25 medium is superior (Harvey & Price, 1983), the majority of studies have shown RV medium to be at least as efficient as R25 (Fricker, Girdwood & Munro, 1983;Fricker, 1984a;Vassiliadis et al 1979) and in some cases to be significantly better (Vassiliadis et al 1984;Fricker & Girdwood, 1985).…”

Section: Introductionmentioning

confidence: 99%

“…In comparisons of the efficiency of RV medium containing soya peptone (RV-soya) with that of the standard RV medium, we have demonstrated that RV-soya is a marginally better medium (Fricker & Girdwood, 1985;McGibbon, Quail & Fricker, 1984).…”

Section: Introductionmentioning

confidence: 99%

An evaluation of commercially available dehydrated Rappaport-Vassiliadis medium for the isolation of salmonellae from poultry

Fricker¹,

Quail²,

McGibbon³

et al. 1985

J. Hyg.

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SUMMARYA total of 745 samples of chicken giblets was cultured to determine the relative efficiency of a commercially available Rappaport-Vassiliadis medium (RV-Oxoid). Experiments to determine the optimum inoculation ratio showed that 1: 100 was superior to the other ratios tested. Comparison of RV-Oxoid with standard RV and RV-medium prepared using soya peptone (RV-soya) showed that after 24 h RV-soya was significantly better than RV-Oxoid (P < 0-05), although there was no significant difference between standard RV and RV-Oxoid. Furthermore, when the duration of incubation was extended to 48 h there was no significant difference between the three media (P > 0 25).We conclude that RV-Oxoid is a satisfactory product for the isolation of salmonellae from poultry, providing that it is inoculated at a ratio of 1: 100 and is incubated for 48h. Its use can therefore be recommended to laboratories who wish to use a dehydrated medium.

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A note on the isolation of salmonellas from environmental samples using three formulations of Rappaport's broth

Cited by 12 publications

References 14 publications

Evaluation of two assays, MSRV and RV, for the isolation of Salmonella spp. from wastewater samples and broiler chickens

Evaluation of two assays, MSRV and RV, for the isolation of Salmonella spp. from wastewater samples and broiler chickens

Selective action of inhibitors used in different culture media on the competitive microflora of Salmonella

An evaluation of commercially available dehydrated Rappaport-Vassiliadis medium for the isolation of salmonellae from poultry

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