A note on the repair of the <i>Escherichia coli</i> nucleoid structure after heat shock

Pellón, J R

doi:10.1111/j.1365-2672.1983.tb02640.x

Cited by 3 publications

(1 citation statement)

References 3 publications

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“…A careful survey of their functions implied that these proteins might have been activated by PEF for different reasons. The outer membrane protein F (ompF; W9ADK8) directly contributes to pore formation and has been shown to help E. coli cells maintain their structural stability (Nogami and Mizushima, 1983). However, the effects of ompF seem to be mixed, as Tan et al (2017) reported that its deletion led to substantial enhancement of membrane integrity.…”

Section: Discussionmentioning

confidence: 99%

Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field

et al. 2019

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The pulsed electric field (PEF) technology has been widely applied to inactivate pathogenic bacteria in food products. Though irreversible pore formation and membrane disruption is considered to be the main contributing factor to PEF’s sterilizing effects, the exact molecular mechanisms remain poorly understood. In this study, by using mass spectrometry (MS)-based label-free quantitative proteomic analysis, we compared the protein profiles of PEF-treated and untreated Escherichia coli. We identified a total of 175 differentially expressed proteins, including 52 candidates that were only detected in at least two of the three samples in one experiment group but not in the other group. Functional analysis revealed that the differential proteins were primarily involved in the regulation of cell membrane composition and integrity, stress response, as well as various metabolic processes. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis was conducted on the genes of selected differential proteins at varying PEF intensities, which were known to result in different cell killing levels. The qRT-PCR data confirmed that the proteomic results could be reliably used for further data interpretation, and that the changes in the expression levels of the differential candidates were, to a large extent, caused directly by the PEF treatment. The findings of the current study offered valuable insight into PEF-induced cell inactivation.

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Section: Discussionmentioning

confidence: 99%

Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field

et al. 2019

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Factors That Affect Microbial Growth in Food

Banwart

1989

Basic Food Microbiology

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Survival and activity of Klebsiella pneumoniae at super-optimal temperatures

Mason

Hamer

1987

Bioprocess Engineering

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The effect of temperature on a population of Klebsiella pneumoniae was examined together with the imposition of mild starvation conditions at temperatures of 35°, 41 °, 49°, 55° and 60 °C. Results for changes in biomass, protein and metabolic activity are presented in terms of gross popuiation changes and show that these parameters decline with increasing temperature. Increases in the amount of dissolved organic carbon, together with a decrease in the number of cells present with increasing temperature, suggest that death and ly~is processes are, occurring. Regrowth in the ,bioreactor occurred after returning the tempera,ture to 35 ° C and starting a flow of carbon and other nutrients. This was probably due to reinoculation from head space wall growth drainage rather than reversion of heat-stressed microbes. The consequences of this for thermophilic sludge treatment processes are discussed. The concept of endogenous metabolism is questioned with respect to it being a realistic description of the survival process.

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A note on the repair of the Escherichia coli nucleoid structure after heat shock

Cited by 3 publications

References 3 publications

Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field

Proteomics-Based Mechanistic Investigation of Escherichia coli Inactivation by Pulsed Electric Field

Factors That Affect Microbial Growth in Food

Survival and activity of Klebsiella pneumoniae at super-optimal temperatures

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