A Novel 9.4 Tesla FTICR Mass Spectrometer with Improved Sensitivity, Mass Resolution, and Mass Range

Kaiser, Nathan K.; Quinn, John; Blakney, Greg T.; Hendrickson, Christopher L.; Marshall, Alan G.

doi:10.1007/s13361-011-0141-9

Cited by 207 publications

(209 citation statements)

References 55 publications

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“…38,39,43 The latter procedures are typically applied to soil, plant or algal organic matter, but were used here to characterize the sand patty-derived DOM. Clearly, the organic matter in sand patties However, there is only a 24% degree of similarity between the plots depicted in Figure 4A and 4C.…”

Section: Chemical Characterization Of Dom Biodegradationmentioning

confidence: 99%

Impact of Photooxidation and Biodegradation on the Fate of Oil Spilled During the Deepwater Horizon Incident: Advanced Stages of Weathering

Harriman

Zito

Podgorski

et al. 2017

Environ. Sci. Technol.

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While the biogeochemical forces influencing the weathering of spilled oil have been investigated for decades, the environmental fate and effects of “oxyhydrocarbons” in sand patties deposited on beaches are not well-known. We collected sand patties deposited in the swash zone on Gulf of Mexico beaches following the Deepwater Horizon oil spill. When sand patties were exposed to simulated sunlight, a larger concentration of dissolved organic carbon was leached into seawater than the corresponding dark controls. This result was consistent with the general ease of movement of seawater through the sand patties as shown with a 35SO4 2– radiotracer. Ultrahigh-resolution mass spectrometry, as well as optical measurements revealed that the chemical composition of dissolved organic matter (DOM) leached from the sand patties under dark and irradiated conditions were substantially different, but neither had a significant inhibitory influence on the endogenous rate of aerobic or anaerobic microbial respiratory activity. Rather, the dissolved organic photooxidation products stimulated significantly more microbial O2 consumption (113 ± 4 μM) than either the dark (78 ± 2 μM) controls or the endogenous (38 μM ± 4) forms of DOM. The changes in the DOM quality and quantity were consistent with biodegradation as an explanation for the differences. These results confirm that sand patties undergo a gradual dissolution of DOM in both the dark and in the light, but photooxidation accelerates the production of water-soluble polar organic compounds that are relatively more amenable to aerobic biodegradation. As such, these processes represent previously unrecognized advanced weathering stages that are important in the ultimate transformation of spilled crude oil.

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Section: Chemical Characterization Of Dom Biodegradationmentioning

confidence: 99%

Impact of Photooxidation and Biodegradation on the Fate of Oil Spilled During the Deepwater Horizon Incident: Advanced Stages of Weathering

Harriman

Zito

Podgorski

et al. 2017

Environ. Sci. Technol.

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“…histone samples were dissolved in 50% acetonitrile (H 2 O/CH 3 CN) (50/50) with 1.0% formic acid to reach a final concentration of 1.0 M and introduced into a custom-built 9.4 Tesla FT-ICR mass spectrometer via positive electrospray ionization (44). Both precursor and product ion spectra were acquired by direct infusion at the flow rate of 0.35 l/min.…”

Section: T Ft-icr Ms-preparedmentioning

confidence: 99%

“…However, the intact experimental mass for the first peak does not agree with the calculated H2B1C mass; thus, producing a false positive result, which we eliminate. ProSight Lite was conducted at the same time to validate our identifications (44).…”

Section: Transformation Of Cells By Low-dose Chronic Exposure Tomentioning

confidence: 99%

Quantitative Mass Spectrometry Reveals Changes in Histone H2B Variants as Cells Undergo Inorganic Arsenic-Mediated Cellular Transformation

Rea

Eleazer

Eckstein

et al. 2016

Molecular & Cellular Proteomics

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Exposure to inorganic arsenic, a ubiquitous environmental toxic metalloid, leads to carcinogenesis. However, the mechanism is unknown. Several studies have shown that inorganic arsenic exposure alters specific gene expression patterns, possibly through alterations in chromatin structure. While most studies on understanding the mechanism of chromatin-mediated gene regulation have focused on histone post-translational modifications, the role of histone variants remains largely unknown. Incorporation of histone variants alters the functional properties of chromatin. To understand the global dynamics of chromatin structure and function in arsenic-mediated carcinogenesis, analysis of the histone variants incorporated into the nucleosome and their covalent modifications is required. Here we report the first global mass spectrometric analysis of histone H2B variants as cells undergo arsenic-mediated epithelial to mesenchymal transition. We used electron capture dissociation-based top-down tandem mass spectrometry analysis validated with quantitative reverse transcription real-time polymerase chain reaction to identify changes in the expression levels of H2B variants in inorganic arsenic-mediated epithelial-mesenchymal transition. We identified changes in the expression levels of specific histone H2B variants in two cell types, which are dependent on dose and length of exposure of inorganic arsenic. In particular, we found increases in H2B variants H2B1H/1K/1C/1J/1O and H2B2E/2F, and significant decreases in H2B1N/1D/1B as cells undergo inorganic arsenic-mediated epithelial-mesenchymal transition. The analysis of these histone vari-

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“…). The experiments were performed with a custom-built 9.4 T ESI FT-ICR mass spectrometer recently described in detail [54]. Samples were infused into a tapered 50 μm i.d.…”

Section: Theorymentioning

confidence: 99%

Relative Stability of Peptide Sequence Ions Generated by Tandem Mass Spectrometry

Bythell

Hendrickson

Marshall

2012

J. Am. Soc. Mass Spectrom.

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We report the use of unimolecular dissociation by infrared radiation for gaseous multiphoton energy transfer to determine relative activation energy (E a,laser ) for dissociation of peptide sequence ions. The sequence ions of interest are mass-isolated; the entire ion cloud is then irradiated with a continuous wave CO 2 laser, and the first order rate constant, k d , is determined for each of a series of laser powers. Provided these conditions are met, a plot of the natural logarithm of k d versus the natural logarithm of laser power yields a straight line, whose slope provides a measure of E a,laser . This method reproduces the E a values from blackbody radiative dissociation (BIRD) for the comparatively large, singly and doubly protonated bradykinin ions (nominally y 9 and y 9 2+ ). The comparatively small sequence ion systems produce E a,laser values that are systematic underestimates of theoretical barriers calculated with density functional theory (DFT). However, the relative E a,laser values are in qualitative agreement with the mobile proton model and available theory. Additionally, novel protonated cyclic-dipeptide (diketopiperazine) fragmentation reactions are analyzed with DFT. FT-ICR MS provides access to sequence ions generated by electron capture dissociation, infrared multiphoton dissociation, and collisional activation methods (i.e., b n , y m , c n , z m• ions).

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A Novel 9.4 Tesla FTICR Mass Spectrometer with Improved Sensitivity, Mass Resolution, and Mass Range

Cited by 207 publications

References 55 publications

Impact of Photooxidation and Biodegradation on the Fate of Oil Spilled During the Deepwater Horizon Incident: Advanced Stages of Weathering

Impact of Photooxidation and Biodegradation on the Fate of Oil Spilled During the Deepwater Horizon Incident: Advanced Stages of Weathering

Quantitative Mass Spectrometry Reveals Changes in Histone H2B Variants as Cells Undergo Inorganic Arsenic-Mediated Cellular Transformation

Relative Stability of Peptide Sequence Ions Generated by Tandem Mass Spectrometry

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