A Novel Acetylcholine Bioensor and Its Electrochemical Behavior

Wang, Wei; Deng, Yan; Li, Song; Liu, Hongna; Lu, Zhuoxuan; Zhang, Liming; Lin, Lin; Xu, Lijian

doi:10.1166/jbn.2013.1577

Cited by 28 publications

(9 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Biosensor is an analysis device consisting of a biosensor and transducer. It mainly uses antigens (antibodies), various sensitive enzymes, alkaloids, and gene sequences to detect microorganisms ( Shiba 2006 ; Wang et al, 2013 ; Liu et al, 2017c ; Ma et al, 2020b ; Guo et al, 2021 ; Das and Mishra, 2022 ). When the sample to be tested reacts with the above substances, biological interactions will occur, which can then be converted into measurable electrical signals by signal transducers, which can be read and detected by signal amplifiers ( Lai et al, 2018a ; Lai et al, 2018b ; Huang et al, 2021 ).…”

Section: Biosensor Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

The global burden of foodborne disease is enormous and foodborne pathogens are the leading cause of human illnesses. The detection of foodborne pathogenic bacteria has become a research hotspot in recent years. Rapid detection methods based on immunoassay, molecular biology, microfluidic chip, metabolism, biosensor, and mass spectrometry have developed rapidly and become the main methods for the detection of foodborne pathogens. This study reviewed a variety of rapid detection methods in recent years. The research advances are introduced based on the above technical methods for the rapid detection of foodborne pathogenic bacteria. The study also discusses the limitations of existing methods and their advantages and future development direction, to form an overall understanding of the detection methods, and for point-of-care testing (POCT) applications to accurately and rapidly diagnose and control diseases.

show abstract

Section: Biosensor Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…5, the axial stiffness of the new pipetting device is represented by a spring constant K 1 and is calculated as per Eq. (1) (Wang et al, 2013).…”

Section: Stiffness Analysis 221 Calculation Of Axial Stiffnessmentioning

confidence: 99%

“…According to the torsion theory, the torsional stiffness of the tip insertion unit can be expressed by the constant K TW and can be calculated as per Eq. (11) (Wang et al, 2013).…”

Section: Torsional Stiffnessmentioning

confidence: 99%

“…The SF3 automatic pipetting station independently developed by the Shanghai Utrao Medical Instrument Co., Ltd. fails to complete the assembly of all 96 pipette tips (Liu et al, 2012). The EpMotion85075 TMX recently rolled out by Eppendorf Switzerland can be used for complex purposes such as multiple proportions of dilution, random sample application, cell culture and nucleic acid isolation and purification by the paramagnetic particle method (Wu et al, 2016). The Freedom EVO8 automatic liquid handling workstation, developed by TECAN Switzerland, provides four workbenches of different capacities, including 75, 100, 150 and 200 cm.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Structure optimization of a pipetting device to improve the insertion effect of tips

Huang

Wang

et al. 2021

Mech. Sci.

View full text Add to dashboard Cite

Abstract. In order to solve the problems of the failure of disposable tip insertion which happens in the pipetting process of most multi-station and high-throughput pipetting devices, this paper proposes a high-rigidity screw-type pipette shaft–disposable tip assembly mechanism with excellent auto-centering effects based on the principle of the ball screw drive. The stiffness model of the new pipetting device is established, and its stiffness and axial deformation are analyzed. This new mechanism was introduced to a multi-station and high-throughput pipetting workstation, and the process of pipetting disposable tips is simulated by ANSYS software. The analytical results show that the stiffness value of the new pipetting device is approximately 90 N/µm, and the amount of deformation of the z-axis manipulator is reduced by about 60 % compared to the original pipetting device. Finally, physical verification of the prototype was carried out in the work. The test results show that the new pipetting workstation can increase the tightening rate of the tips by approximately 12 % after optimization when 96 tips are inserted in a single press. In addition, the pass rate of the tightness test of the optimized pipetting workstation has increased by approximately 20 %.

show abstract

“…15 18-20 These are promising techniques that could be applied in many fields such as in the diagnosis of infectious diseases, the monitoring of the environment and in the detection of potential bioterrorism agents. [21][22][23][24][25] Mast cells have excellent potential in biosensor applications because they undergo a dramatic activation in response to antigenic stimulation within minutes. Allergens bind specifically to immunoglobulin E (IgE), which is linked to the high-affinity receptor for IgE (Fc RI) leading to the crosslinking of membranous Fc RI, and consequently stimulation of a series of cellular events.…”

Section: Introductionmentioning

confidence: 99%

Development of Aequorin-Based Mast Cell Nanosensor for Rapid Identification of Botulinum Neurotoxin Type B

Xin¹,

Yao²,

Gao³

et al. 2014

j biomed nanotechnol

View full text Add to dashboard Cite

An aequorin-based mast cell sensor was developed for rapid identification and detection of protein toxins. To monitor mast cell activation and to improve the sensitivity of detection, aequorin was stably expressed in the cells and used as an indicator of calcium flux and the stable cell line was created. The procedures for preparing cells were optimized to improve the quantum yield and sensitivity of detection. The cells sensitized with anti-DNP (dinitrophenyl) IgE were capable of detecting as little as 0.1 ng/mL DNP-BSA in less than 2 min. To demonstrate the utility of this mast cell sensor in detecting protein toxins, an IgE chimeric protein (p21-Fc was created. This was achieved by fusing the Fc region of IgE antibody to a 21-amino acid peptide (p21) derived from residues 40-60 of synaptotagmin II (Syt II), the protein receptor of botulinum neurotoxin type B (BoNT/B). In addition, magnetic beads linked with anti-BoNT/B polyclonal antibody were prepared to capture BoNT/B molecules in solution to make targets multivalent and concentrated. The p21-Fc binds to Fc RI receptors on RBL (Rat basophilic leukemia) cells and can be cross-linked by BoNT/B captured by the magnetic beads. This led to cell activation and Ca 2+ flux indicated by an increase of quantum yield. This assay can detect as little as 100 pM (15 ng/mL) BoNT/B in less than 1 hr, which included the initial sample preparation time. This study lays a foundation for detecting other protein toxins using mast cell sensors.

show abstract

A Novel Acetylcholine Bioensor and Its Electrochemical Behavior

Cited by 28 publications

References 15 publications

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Structure optimization of a pipetting device to improve the insertion effect of tips

Development of Aequorin-Based Mast Cell Nanosensor for Rapid Identification of Botulinum Neurotoxin Type B

Contact Info

Product

Resources

About