A novel acid phosphatase from cactus (Opuntia megacantha Salm-Dyck) cladodes: Purification and biochemical characterization of the enzyme

Chafik, Abdelbasset; Essamadi, Abdelkhalid; Çelik, Safinur Yıldırım; Mavi, Ahmet

doi:10.1016/j.ijbiomac.2020.05.175

Cited by 15 publications

(9 citation statements)

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“…Effects of metal ions (K + , Co 2+ , Ni + , Mg 2+ , Mn 2+ , Ca 2+ , Ba 2+ , Fe 3+ and Cu 2+ ) on CB6-C antibacterial activity were carried out according to the protocol described previously [ 13 ]. Briefly, a 512 ug/mL of CB6-C was continuously twofold diluted to 1ug/mL and added metal ions to CB6-C diluents of different concentrations to make a final concentration of metal ions in each of a 10 mM.…”

Section: Methodsmentioning

confidence: 99%

Discovery of a novel antibacterial protein CB6-C to target methicillin-resistant Staphylococcus aureus

et al. 2022

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Given a serious threat of multidrug-resistant bacterial pathogens to global healthcare, there is an urgent need to find effective antibacterial compounds to treat drug-resistant bacterial infections. In our previous studies, Bacillus velezensis CB6 with broad-spectrum antibacterial activity was obtained from the soil of Changbaishan, China. In this study, with methicillin-resistant Staphylococcus aureus as an indicator bacterium, an antibacterial protein was purified by ammonium sulfate precipitation, Sephadex G-75 column, QAE-Sephadex A 25 column and RP-HPLC, which demonstrated a molecular weight of 31.405 kDa by SDS-PAGE. LC–MS/MS analysis indicated that the compound was an antibacterial protein CB6-C, which had 88.5% identity with chitosanase (Csn) produced by Bacillus subtilis 168. An antibacterial protein CB6-C showed an effective antimicrobial activity against gram-positive bacteria (in particular, the MIC for MRSA was 16 μg/mL), low toxicity, thermostability, stability in different organic reagents and pH values, and an additive effect with conventionally used antibiotics. Mechanistic studies showed that an antibacterial protein CB6-C exerted anti-MRSA activity through destruction of lipoteichoic acid (LTA) on the cell wall. In addition, an antibacterial protein CB6-C was efficient in preventing MRSA infections in in vivo models. In conclusion, this protein CB6-C is a newly discovered antibacterial protein and has the potential to become an effective antibacterial agent due to its high therapeutic index, safety, nontoxicity and great stability.

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Section: Methodsmentioning

confidence: 99%

Discovery of a novel antibacterial protein CB6-C to target methicillin-resistant Staphylococcus aureus

et al. 2022

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“…Mn 2+ ions inhibited some APases (Sugiura et al, 1981;Guo & Pesacreta, 1997;Yenigun & Guvenilir, 2003;Garcia et al, 2004;Anand & Srivastava, 2013). Fe 3+ also inhibited APase isoforms from Vigna species (V. sinensis and V. aconitifolia) and Opuntia (Biswas & Cundiff, 1991;Biswas et al, 1996;Anand & Srivastava, 2013;Chafik, 2020). Co 2+ inhibited some APases (Turner & Plaxton, 2001;Garcia et al, 2004).…”

Section: A Effect Of Cationsmentioning

confidence: 99%

“…Acid phosphatase (EC 3.1.3.2) (APase) catalyzes the hydrolysis of phosphate esters to yield alcohol and inorganic phosphate at pH below 7.0 (Duff et al, 1994). APase got isolated and characterized from different plant sources (Roknabadi et al, 1999;Turner & Plaxton, 2001;Koffi et al, 2010;Anand & Srivastava, 2013;Tagad & Sabharwal, 2018;Zaman et al, 2020;Chafik et al, 2020;Nongpiur et al, 2021). Reports are there on the influence of ions (cations and anions) and metabolites (effectors) on the acid phosphatase activity (Guo & Roux, 1995;Turner & Plaxton, 2001;Coello, 2002;Greiner & Jany, 2003;Koffi et al, 2010;Nadir et al, 2012;Khan et al, 2016;Chafik, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Rice Bean (Vigna umbellata Thunb.) Acid Phosphatase: Effect of Metal Ions and Metabolites

Nongpiur,

Ambasht

2022

JSR

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Rice bean acid phosphatase dialyzed against ethylenediaminetetracetic acid (1 mM), yielded no loss in activity at 0.5 mM p-nitrophenylphosphate. It had a mild activating effect at 0.1 mM p-nitrophenylphosphate. Divalent cations belonging to group II of Periodic Table like Mg2+, Ca2+, Sr2+, and Ba2+ at 0.5 mM concentration p-nitrophenylphosphate did not affect the enzyme activity. At 0.1 mM p-nitrophenylphosphate, however, only Mg2+ ions showed little inhibition. The transition metal ions viz. Zn2+ , Cu2+, Co2+, Mn2+ and Fe3+ showed inhibition at both pnitrophenylphosphate concentrations. Na+ , K+ , and NH4 + ions did not influence the enzyme activity, but Li+ inhibited the activity at 0.1 and 0.5 mM p-nitrophenylphosphate concentrations, respectively. Molybdate and phosphate anions proved to be strong inhibitors, but vanadate, a moderate inhibitor. Tartrate anion did not exhibit an effect on rice bean acid phosphatase. Sugars, plant hormones, medicines, vitamins, and amino acids at 1.0 mM concentration did not affect the enzyme activity. NADH exhibited the maximum activation, followed by citric acid, isocitrate, and oxaloacetate. Caffeine showed activation in the enzyme activity. Phosphate esters (1-Naphthylphosphate, phenylphosphate, PEP, and ADP) exhibited competitive inhibition when pnitrophenylphosphate was used as a substrate. Non-ionic detergents Triton X-100 and Tween-20 brought activation to the enzyme. The ionic detergent SDS brought complete loss to the enzyme activity. Dithiothreitol brought inhibition to the enzyme activity. The presence of β-mercaptoethanol did not influence the enzyme activity.

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“…Effects of metal ions (K + , Co 2+ , Ni + , Mg 2+ , Mn 2+ , Ca 2+ , Ba 2+ , Fe 3+ and Cu 2+ ) on CB6-C antibacterial activity were carried out according to the protocol described previously [14]. Brie y, a 512 ug/mL of CB6-C was continuously 2-fold diluted to 1ug/mL and added metal ions to CB6-C diluents of different concentrations to make a nal concentration of metal ions in each of a 10 mM.…”

Section: Effect Of Metal Ion On Cb6-c Activitymentioning

confidence: 99%

Discovery of a Novel Antibacterial Protein CB6-C to Target Methicillin-Resistant Staphylococcus Aureus

Zhang

Liu

Inam

et al. 2021

Preprint

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Given the serious threat of multidrug-resistant bacterial pathogens to global healthcare, there is an urgent need to find effective antibacterial compounds to treat drug-resistant bacterial infections. In our previous studies, Bacillus velezensis CB6 with broad-spectrum antibacterial activity was obtained from the soil of Changbaishan, China. In this study, with MRSA as an indicator bacterium, an antibacterial protein was purified by ammonium sulfate precipitation, a Sephadex G-75 column, a QAE-Sephadex A 25 column and RP-HPLC, which demonstrated a molecular weight of 31405.48 Da by SDS-PAGE. LC-MS/MS analysis indicated that the compound was an antibacterial protein CB6-C, which had 88.5% homology with chitosanase Csn produced by Bacillus subtilis 168. An antibacterial protein CB6-C showed an effective antimicrobial activity against gram-positive bacteria (in particular, the MIC for MRSA was 16 μg/mL), low toxicity, thermostability, stability in different organic reagents and pH values, and an additive effect with conventionally used antibiotics. Mechanistic studies showed that an antibacterial protein CB6-C exerted anti-MRSA activity through destruction of lipoteichoic acid (LTA) on the cell wall. In addition, an antibacterial protein CB6-C was efficient in preventing MRSA infections in In Vivo models. In conclusion, this protein CB6-C is a newly discovered antibacterial protein and has the potential to become an effective antibacterial agent due to its high therapeutic index, safety, nontoxicity and great stability.

show abstract

A novel acid phosphatase from cactus (Opuntia megacantha Salm-Dyck) cladodes: Purification and biochemical characterization of the enzyme

Cited by 15 publications

References 50 publications

Discovery of a novel antibacterial protein CB6-C to target methicillin-resistant Staphylococcus aureus

Discovery of a novel antibacterial protein CB6-C to target methicillin-resistant Staphylococcus aureus

Rice Bean (Vigna umbellata Thunb.) Acid Phosphatase: Effect of Metal Ions and Metabolites

Discovery of a Novel Antibacterial Protein CB6-C to Target Methicillin-Resistant Staphylococcus Aureus

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