2010
DOI: 10.2478/v10007-010-0035-6
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A novel analytical approach for reducing the consumption of organic solvents in the charge transfer-based spectrophotometric analysis: Application in the analysis of certain antihypertensive drugs

Abstract: The present study describes the development of a novel analytical approach that can reduce by 50-fold the consumption of organic solvents in the charge transfer (CT)--based spectrophotometric analysis. The proposed approach employed 96-microwell assay plates for carrying out the reaction. The CT reaction between the electron--donating analyte and electron-accepting reagent was performed in microwells (200-mL of organic solvent) and the color signals were measured with a microwell-plate reader. Optimum conditio… Show more

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Cited by 16 publications
(11 citation statements)
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“…The solutions were dispensed by 8-channel pipette, and the absorbances of the colored CT complex were measured by 96-microwell-plate absorbance reader. The 96-microwell design of the proposed assay was based on the previous success of Darwish et al [28] in the utility of this design for determination of some other pharmaceuticals.…”
Section: Resultsmentioning
confidence: 99%
“…The solutions were dispensed by 8-channel pipette, and the absorbances of the colored CT complex were measured by 96-microwell-plate absorbance reader. The 96-microwell design of the proposed assay was based on the previous success of Darwish et al [28] in the utility of this design for determination of some other pharmaceuticals.…”
Section: Resultsmentioning
confidence: 99%
“…For these reasons, DDQ was used as electron acceptor in the development of the proposed assay. The 96-microwell design of the proposed assay was based on the previous success of Darwish et al [27] in the utility of this design for determination of some other pharmaceuticals.…”
Section: Resultsmentioning
confidence: 99%
“…For these reasons, DDQ was used as electron acceptor in the development of the proposed assay. The 96-microwell design of the proposed assay was based on the previous success of Darwish et al [31] in the utility of this design for determination of some other pharmaceuticals.…”
Section: Resultsmentioning
confidence: 99%