A Novel and Effective Strategy for the Isolation of Adipose-Derived Stem Cells

Raposio, Edoardo; Caruana, Giorgia; Bonomini, Sabrina; Libondi, Guido

doi:10.1097/prs.0000000000000170

Cited by 92 publications

(58 citation statements)

References 4 publications

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“…[76], [77], [78] described a method that was specifically designed for clinical application, which appeared easy, safe and fast (80 min), allowing collection of a ready-to-use ASC pellet. Just like previous isolation processes, first the adipose tissue was harvested by a standard liposuction procedure, and then the isolation process was carried out through both mechanical (centrifugation) and enzymatic (collagenase) means [78].…”

Section: Adipose-derived Stem Cells (Ascs)mentioning

confidence: 99%

“…However, the clinical application of ASCs isolated using mechanical means during the same operative session falls under the jurisdiction of medical practice and is thus allowed. Therefore, the development of high-yield isolation processes for ASCs with minimal handling would be highly desirable for clinical applications [76].…”

Section: Adipose-derived Stem Cells (Ascs)mentioning

confidence: 99%

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The biological and clinical basis for the use of adipose-derived stem cells in the field of wound healing

Bertozzi

Simonacci

Grieco

et al. 2017

Annals of Medicine &Amp; Surgery

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Worldwide, hard-to-heal lower limb wounds are estimated to affect 1.5–3% of the adult population with a treatment-related annual cost of $10 billion. Thus, chronic skin ulcers of the lower limb are a matter of economic and public concern. Over the years, multiple medical and surgical approaches have been proposed but they are still inadequate, and no effective therapy yet exists. Regenerative medicine and stem cell-based therapies hold great promise for wound healing. Recently, many plastic surgeons have studied the potential clinical application of adipose-derived stem cells (ASCs), which are a readily available adult stem cell population that can undergo multilineage differentiation and secrete growth factors that can enhance wound-healing processes by promoting angiogenesis, and hence increase local blood supply. ASCs have been widely studied in vitro and in vivo in animal models. However, there are few randomized clinical trials on humans, and these are still ongoing or recruiting patients. Moreover, there is no consensus on a common isolation protocol that is clinically feasible and which would ensure reproducible results. The authors aim to provide readers with an overview of the biological properties of ASCs as well as their clinical application, to help better understanding of present and future strategies for the treatment of hard-to-heal wounds by means of stem cell-based therapies.

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Section: Adipose-derived Stem Cells (Ascs)mentioning

confidence: 99%

Section: Adipose-derived Stem Cells (Ascs)mentioning

confidence: 99%

The biological and clinical basis for the use of adipose-derived stem cells in the field of wound healing

Bertozzi

Simonacci

Grieco

et al. 2017

Annals of Medicine &Amp; Surgery

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“…Constricted skin with a very thin or scarred subcutaneous fat layer cannot sustain as large an amount of fat as healthy tissue, preferably with lax skin and a thick subcutaneous fat layer [88]. In fact, the vitality of ASCs has proven to be crucial for stimulating tissue regeneration through the chemotactic, paracrine, and immunomodulatory activities of the cells and in situ differentiation [38], [39].…”

Section: Discussionmentioning

confidence: 99%

“…Stem cells isolated from lipoaspirates have demonstrated a broad in vitro adipogenic, chondrogenic, osteogenic, and myogenic lineage commitment [33], [34] as well as differentiation into pancreatic cells, hepatocytes, and neurogenic cells [35], [36], [37]. Cytometric analysis of adipose-derived stem cells (ASCs) has shown that these cells do not express CD31 and CD45, but do express CD34, CD73, CD105, and the mesenchymal stem cell marker CD90 [38], [39]. ASCs have a differentiation potential similar to that of other mesenchymal stem cells as well as a higher yield upon isolation and a greater proliferative rate in culture than bone marrow–derived stem cells [40], [41], [42].…”

Section: Efficacy Of Lipofillingmentioning

confidence: 99%

Autologous fat transplantation for breast reconstruction: A literature review

Simonacci

Bertozzi

Grieco

et al. 2016

Annals of Medicine &Amp; Surgery

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IntroductionThe use of autologous fat transplantation to correct volume and contour defects, scars, and asymmetry after breast cancer surgery has increased over the past 20 years. Many developments and refinements in this technique have taken place in recent years, and several studies of the safety of lipofilling in the breast have been published.Presentation of caseWe performed a literature review of this technique, highlighting the crucial role of lipofilling in breast cancer reconstruction.DiscussionThe efficacy of the fat graft transplantation depends on the experience and the technique used by the surgeon. The ASCs (adipose-derived stem cells) contained in the fat graft has proven to be crucial for breast reconstruction by mean the regeneration of tissue, through the chemotactic, paracrine, and immunomodulatory activities and their in situ differentiation.ConclusionThe role of lipofilling for breast reconstruction could be more significant with the application of the findings of experimental research on tissue engineering and ASCs.

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“…Newer methods claim quicker and cheaper isolation [40], and some attempt to bypass enzyme digestion all together [53]. Because a key step in adipose-derived stem cell retrieval is the isolation of the SVF (the pellet of mixed cell types after centrifugation of collagenase-treated lipoaspirate), many commercial devices have surfaced over the last decade targeting SVF isolation.…”

Section: Asc Isolationmentioning

confidence: 99%

Regenerative Engineering of Cartilage Using Adipose-Derived Stem Cells

Kasir

Vernekar

Laurencin

2015

Regen. Eng. Transl. Med.

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Injury to the articular cartilage occurs commonly in the general population and undergoes minimal spontaneous healing. Traditional methods of cartilage repair provide no long-term cure and are significant causes of morbidity. For this reason, stem cell therapies have recently been investigated for their ability to regenerate cartilage, and the results have been promising. Since the discovery that adipose tissue is a major source of mesenchymal stem cells in 2001, scientists have been studying the use of adipose-derived stem cells (ASCs) for the treatment of various disorders including lesions of the articular cartilage. ASCs hold several advantages over autologous chondrocytes for cartilage repair, including but not limited to their anti-inflammatory effects, their multi-lineage differentiation potential, and their ability to form new cartilage in a defect. Whereas several investigations have been made in in vitro and animal models, there have been surprisingly little clinical studies on the intra-articular use of adipose-derived stem cells, despite their first isolation about a decade and a half ago. The few studies that have been conducted are encouraging. With approval for various stem cell therapies on the horizon, this review seeks to update the clinician and the researcher on the current state-of-the-art use of adipose-derived stem cells for the treatment of cartilage disorders and the regenerative engineering of cartilaginous tissue.

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A Novel and Effective Strategy for the Isolation of Adipose-Derived Stem Cells

Cited by 92 publications

References 4 publications

The biological and clinical basis for the use of adipose-derived stem cells in the field of wound healing

The biological and clinical basis for the use of adipose-derived stem cells in the field of wound healing

Autologous fat transplantation for breast reconstruction: A literature review

Regenerative Engineering of Cartilage Using Adipose-Derived Stem Cells

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