This study presents a wideband CPW fed four-port multi-input multi-output (MIMO) antenna-based microwave sensor for measuring deep brain temperature inside human head. The range of frequency is from 5.45 to 8.56 GHz at the resonance frequency of 7.25 GHz. Coplanar Waveguide (CPW) fed approach on a low-cost FR4 substrate is used in designing the planar antenna. The presented antenna is compact, having overall dimensions of 40 × 40 × 1.6 mm3. In the operational frequency range, the obtained diversity performance for MIMO antenna shows Envelope Correlation Coefficient (ECC) < 0.05, Diversity Gain > 9.95, total active reflection coefficient (TARC ) < −13 dB and channel capacity loss (CCL) < 0.125 bits/S/Hz. For deep brain temperature measurements, the proposed sensor provides electric field of 3.92 V m−1 and specific absorption rate (SAR) value of 0.16 W kg−1 at the 9 mm distance inside the human head Phantom. The proposed sensor is capable of measuring 0.1 °C change in temperature at 10 W of input power for 60 s when all four ports are active.