A novel and efficient bioprocess from steam exploded corn stover to ethanol in the context of on-site cellulase production

Zhao, Chen; Zou, Zongsheng; Li, Jisheng; Jia, Honglei; Liesche, Johannes; Fang, Hao; Chen, Shaolin

doi:10.1016/j.energy.2017.02.041

Cited by 27 publications

(24 citation statements)

References 36 publications

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“…Then FPAs decreased because T. reesei Rut-C30 entered decline phase, which is in line with the previous work 18, 29 . Thus, the cellulases harvested on Day 5 were applied to the enzymatic hydrolysis of themselves in the context of on-site cellulase production 13, 14, 15 , i.e. the cellulase induced by Avicel used for the enzymatic hydrolysis of Avicel, because this is advantageous over the cellulases induced by other substrates or commercial cellulases 15, 30 .…”

Section: Resultsmentioning

confidence: 99%

“…In the scenario of SHF (Fig. 1), the cellulase was produced by T. reesei Rut-C30 from Avicel or steam exploded corn stover (SECS) 14, 24, 25 was applied to the enzymatic hydrolysis of themselves, so called “on-site cellulase production” 13, 14, 15, 25 . T. reesei Rut-C30 was precultured in the seed medium for 36 h and then the seed collected and inoculated into the fermentation medium for cellulase production.…”

Section: Methodsmentioning

confidence: 99%

“…T. reesei Rut-C30 was precultured in the seed medium for 36 h and then the seed collected and inoculated into the fermentation medium for cellulase production. The fermentation broth containing crude cellulase was directly used in the enzymatic hydrolysis of Avicel or SECS 14, 19 , which was operated in 250 mL Erlenmeyer flasks with a working volume of 50 mL 2.5 mL 1 M citrate buffer solution (for final pH 4.8), 50 g/L Avicel or 100 g/L SECS (dry material), 25 FPIU/g glucan the cellulase harvested after 5 d fermentation, and a supplementary amount of water to make up 50 mL. The enzymatic hydrolysis was conducted in a rotary shaker at 50□ with a shaking of 140 rpm for 48 h. The resulted enzymatic hydrolysates containing fermentable sugars were used to comprise of fermentation medium supplemented with the same nutrients as YPD medium except glucose.…”

Section: Methodsmentioning

confidence: 99%

“…1, we applied the engineered strain of S. cerevisiae INVSc1 to bioprocesses for D-glucaric acid production from model cellulose and natural lignocelluloses, the same as biofuel production such as bioethanol, including separated hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP) 9, 10, 11, 12, 13, 14 . SHF and SSF were carried out in the context of on-site cellulase production because this mode has many advantages, enabling cost saving cost and tailor made enzyme for a given feedstock 13, 14, 15 . CBP by an artificial microbial consortium composed of Trichoderma reesei Rut-C30 and the engineered S. cerevisiae strain was established in this study to actualize direct production of D-glucaric acid from lignocelluloses.…”

Section: Introductionmentioning

confidence: 99%

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Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium

Lin

Ling

et al. 2020

Preprint

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The biomanufacturing of D-glucaric acid has been attracted increasing interest and the industrial yeast Saccharomyces cerevisiae is regarded as an excellent host for D-glucaric acid production. Here we constructed the biosynthetic pathway of D-glucaric acid in S. cerevisiae INVSc1 whose opi1 was knocked out and obtained two engineered strains, LGA-1 and LGA-C, producing record breaking titers of D-glucaric acid, 9.53 ± 0.46 g/L and 11.21 ± 0.63 g/L D-glucaric acid from 30 g/L glucose and 10.8 g/L myo-inositol in the mode of fed-batch fermentation, respectively. Due to the genetic stability and the outperformance in subsequent applications, however, LGA-1 was a preferable strain. As one of the top chemicals from biomass, there have been no reports on D-glucaric acid production from lignocellulose, which is the most abundant renewable on earth. Therefore, the biorefinery processes of lignocellulose for D-glucaric acid production including separated hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP) were investigated in this work and CBP by an artificial microbial consortium composed of Trichoderma reesei Rut-C30 and S. cerevisiae LGA-1 was found to have relatively high D-glucaric acid titers and yields after 7 d fermentation, 0.54 ± 0.12 g/L D-glucaric acid from 15 g/L Avicel, and 0.45 ± 0.06 g/L D-glucaric acid from 15 g/L steam exploded corn stover (SECS), respectively. In attempts to design the microbial consortium for more efficient CBP the team consisted of the two members, T. reesei Rut-C30 and S. cerevisiae LGA-1, was found to be the best with excellent work distribution and collaboration.This desirable and promising approach for direction production of D-glucaric acid from lignocellulose deserves extensive and in-depth research.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium

Lin

Ling

et al. 2020

Preprint

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“…Lignocellulose, the main component of the plant cell wall, is the most abundant renewable resource on earth . It is a desirable feedstock for production of biofuels to power this world sustainably as fossil fuels exhaust.…”

Section: Introductionmentioning

confidence: 99%

Heterologous expression of Neurospora crassa cbh1 gene in Pichia pastoris resulted in production of a neutral cellobiohydrolase I

Yang

Deng

Zhao

et al. 2019

Biotechnology Progress

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The high production cost of cellulase is one of the limitations that hinder the commercialization of lignocellulose-based biorefineries. As one of the important cellulases, Neurospora crassa cellulase is not so intensively investigated as T. reesei cellulase.In this study, the cbh1gene (NCU07340) cloned from N. crassa was expressed in Pichia pastoris under the control of alcohol oxidase 1 (AOX1) promotor. Six transformants with the highest resistance to G418 were selected by two rounds of transformant screening, among which the most robust producer of the recombinant cellobiohydrolase I (CBHI) has an Avicelase activity of 0.22 U/mL. After fermentation optimization, it was improved to 0.30 U/mL. Interestingly, the optimal temperature and pH of the recombinant CBHI were 60 C and 7.2, respectively, and it was quite stable within the wide ranges of temperature and pH. This work is a good example for the future improvement and optimization of N. crassa cellulase.

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Distributive and collaborative push‐and‐pull in an artificial microbial consortium for improved consolidated bioprocessing

et al. 2022

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The consolidated bioprocessing (CBP) of lignocellulose by the synthetic microbial consortium of Trichoderma reesei and Saccharomyces cerevisiae is a promising way of biomanufacturing d‐glucaric acid. However, the hindrance factor to its industrial application is the low efficiency. Therefore, we engineered T. reesei and S. cerevisiae to improve the CBP for d‐glucaric acid production. T. reesei was engineered to produce more cellulase and release more fermentable sugars from lignocellulose, that is, pushing more sugars to S. cerevisiae. S. cerevisiae was engineered to metabolize cellobiose and siphon more sugars into d‐glucaric acid biosynthetic pathway, that is, pulling more sugars to S. cerevisiae. This is the strategy of distributive and collaborative push‐and‐pull we developed and proposed in this work, which was proven successful in improving efficiencies of the CBPs of steam‐exploded corn stover (SECS) for d‐glucaric acid production and distiller's grains for single cell protein (SCP) production. The titer, yield and productivity of d‐glucaric acid produced from 50 g/L SECS by the microbial consortium of T. reesei C10 and S. cerevisiae LGA‐1C3S2 were 6.42 g/L, 0.128 g/g SECS, and 0.917 g/L/d, respectively. The titer, yield, and productivity of SCP produced from 80 g/L distiller's grains were 50.5 g/L, 0.631 g/g distiller's grains, and 8.417 g/L/d, respectively. These were much higher than the initial microbial consortia of T. reesei Rut‐C30 and S. cerevisiae LGA‐1 or INVSc1. The results confirmed the applicability and generalizability of distributive and collaborative push‐and‐pull, which has profound meaning for science and engineering.

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A novel and efficient bioprocess from steam exploded corn stover to ethanol in the context of on-site cellulase production

Cited by 27 publications

References 36 publications

Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium

Consolidated bioprocessing of lignocellulose for production of glucaric acid by an artificial microbial consortium

Heterologous expression of Neurospora crassa cbh1 gene in Pichia pastoris resulted in production of a neutral cellobiohydrolase I

Distributive and collaborative push‐and‐pull in an artificial microbial consortium for improved consolidated bioprocessing

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