A Novel and Efficient Colorimetric Assay for Quantitative Determination of Amlodipine in Environmental, Biological and Pharmaceutical Samples

Abstract

Development of facile, on-spot, robust, and efficient optical nanosensors for drugs and pesticides is one of the active research area. In this study, a nanosensor based on fairly stable silver nanoparticles (AgNPs) conjugated with specially designed thiourea (TU)-based molecule, N-((4'-methoxy-2'-nitrophenyl) carbamothioyl) dodecanamide (TU3), was prepared using one pot protocol and characterized by Ultraviolet (UV)-Visible spectroscopy, dynamic light scattering (DLS), fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM). The TU3 conjugated AgNPs (TU3-AgNPs) were found to be selective spectrophotometric sensor for an antimicrobial drug, amlodipine (Ad), among the pool of different drugs. TU3-AgNPs based nanosensor allows for the rapid quantitative assay having a detection limit of 0.3 μM in an analytical range of 0.1-100 μM. The proposed sensor is specific in presence of number of other interfering drugs and other matrix. Upto the best of our literature survey, this is first ever colorimetric sensor for quantification of amlodipine in complex matrix. The specificity of the sensor allows for quantitative detection of amlodipine in environmental, biological and pharmaceutical samples.

Antiurease screening of alkyl chain-linked thiourea derivatives: in vitro biological activities, molecular docking, and dynamic simulations studies

“…N-((4 0 -Methoxy-2 0 -nitrophenyl) carbamothioyl) dode canamide (3a). 20 4 Hz, H-3 0 , H-5 0 ), 7.76 (2H, d, J 2 0 ,3 0 / 6 0 ,5 0 ¼ 8.7 Hz, H-2 0 , H-6 0 ), 2.45 (obscured by DMSO signal, H-2), 1.57 (2H, app q, J 3 ¼ 6.9 Hz, H-3), 1.23 (8H, m), 0.86 (3H, t, J 12 ¼ 6 Hz, H-8); 13 12.41 (1H, s, NH), 11.58 (1H, s, NH), 7.88 (1H, app dd, J 3 0 ,4 0 ¼ 8.0 Hz, J 3 0 ,5 0 ¼ 1.2 Hz, H-3 0 ), 7.71 (1H, app dd, J 6 0 ,5 0 ¼ 8.0 Hz, J 6 0 ,4 0 ¼ 1.2 Hz, H-6 0 ), 7.43 (1H, td, J 5 0 ,(6 0 ,4 0 ) ¼ 8.0 Hz, J 5 0 ,3 0 ¼ 1.2 Hz, H-5 0 ), 7.24 (1H, td, J 4 0 ,(5 0 ,3 0 ) ¼ 8.0 Hz, J 4 0 ,6 0 ¼ 1.2 Hz, H-4 0 ), 2.49 (obscured by DMSO signal, H-2), 1.57 (2H, q, J 3 ¼ 12.0 Hz, H-3), 1.26 (8H, m), 0.87 (3H, t, J 8 ¼ 6.0 Hz, H-8); 13…”

Thiourea-Based Receptors for Anion Recognition and Signaling

“…During the last decades, anion recognition has been a very important area of supramolecular chemistry that is currently growing. , In part, this is because the chemistry involved in this field has diverse applications in different topics such as catalysis, , medicine, − and materials science, among others. − Likewise, anions play a significant role in various environmental processes. ,− Therefore, the design of selective and sensitive receptors is a very important issue as well as the implementation of real-time monitoring methods for these species. However, the recognition of anions remains a challenge due to the intrinsic characteristics of these species, such as geometry, small charge density, high solvation energies, and possible pH dependence, among others. , In this context, in recent decades various approaches to anion recognition and signaling have been addressed, the most common involving hydrogen bond donor groups as receptor units, such as amides, , ureas, , thioureas, , squaramides, − and some combinations of the previous ones, , among others.…”

Simultaneous determination of amlodipine and lisinopril dihydrate using fourth derivative spectroscopy

“…Amlodipine (ALP), 3, 5-Pyridinedicarboxylic acid, 2-[(2-ami noethoxy)methyl]-4-(2-chlorophenyl)-1, 4-dihydro-6-methyl, 3-ethyl 5-methyl ester, is a calcium channel blocker (Scheme 1). It is used to treat chronic angina, hypertension, and myocardial infarction, as well as a specific type of chronic angina [4][5][6]. It prevents coronary arterial contraction and narrowing of the arteries, leading to increased blood flow and myocardial oxygenation [7].…”