A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

Liu, Wei; Shang, Xiang; Yao, Shuyong; Wang, Feng

doi:10.1002/bmc.4695

Cited by 8 publications

(2 citation statements)

References 30 publications

(29 reference statements)

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“…A previous study reported the advantages and characteristics of 2D-LC comparing with others (Yu et al, 2019). Furthermore, 2D-LC has been successfully applied to the determination of vancomycin (Li et al, 2014), propranolol (S. S. Liu et al, 2019), and apatinib (Yu et al, 2019) in the human plasma and valproic acid (W. Liu, Shang, et al, 2020) in the human serum.…”

Section: The Selection Of Detection Methodsmentioning

confidence: 99%

A novel method for the quantification of anlotinib in human plasma using two‐dimensional liquid chromatography

Chen

Shi

et al. 2021

Biomedical Chromatography

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A simple, efficient, and stable detection method of two-dimensional liquid chromatography (2D-LC) was established and validated to determine anlotinib in the human plasma. The 2D-LC system comprises a first-dimensional column (LC1), an intermediate transfer column, and a second-dimensional column (LC2). With simple protein precipitation treatment, the samples were processed directly for detection. The analysis cycle time was completed within 9.50 min. For the anlotinib concentrations, the calibration curve was linear over the 5.00-320.00 ng/mL range. The intra-day and inter-day precision ranges were 0.77-6.22% and 1.92-4.26%, respectively, for anlotinib concentrations. The recoveries were in the range of 97.85-102.50%. A total of 135 plasma samples from 94 patients were analyzed by our method. The plasma concentrations of patients were in the range of 5.17-106.38 ng/mL, in which the female had a higher plasma concentration (6.44-106.38 ng/mL). The simultaneous application of dexamethasone can increase the anlotinib concentration in the plasma.In our clinical application, we found that the factors that affect the plasma concentration include the time and dose of the medication, gender, and drug interactions. The method appears to be sensitive, precise, selective, and suitable for determining the concentration of anlotinib in the plasma sample.

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Section: The Selection Of Detection Methodsmentioning

confidence: 99%

A novel method for the quantification of anlotinib in human plasma using two‐dimensional liquid chromatography

Chen

Shi

et al. 2021

Biomedical Chromatography

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“…The targets were transferred from the 1 D column to the middle column and then to the 2 D column in the 2D-LC system; therefore, the transfer recovery could impact the accuracy and precision. It was necessary to evaluate the transfer recovery to determine whether the targets were completely transported in the system (Liu et al, 2019).…”

Section: Transfer Recoverymentioning

confidence: 99%

A novel and sensitive method for determination of amisulpride in human plasma by two‐dimensional liquid chromatography

Wang

et al. 2021

Biomedical Chromatography

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A novel and sensitive heart-cutting two-dimensional liquid chromatography with ultraviolet detection method (2D-LC-UV) was developed and validated for determination of amisulpride in human plasma. The 2D-LC system consists of a first dimensional ( 1 D) LC column and a middle transfer column as well as a second-dimensional ( 2 D) LC column. After simple protein precipitation, the sample was directly injected into the introduction valve of the 2D-LC system. The 1 D column, playing a role of primary separation and preconcentration for complex plasma matrices, transferred the targets to the intermediate column. Following capture of targets on the middle column online, the analytes were transferred to the 2 D separation column by a six-port valve. The 2 D column, avoiding interference from the plasma matrix, completed further separation and quantification. An assistant pump was optimized for primary enrichment as well as final elution in the heart-cutting mode. The analytical time of amisulpride was 7.401 min. The accuracy was between 0.48 and 8.49%, while the intra-and inter-day precisions ranged from 0.9 to 3.1% and from 1.7% to 3.3%, respectively. The linear range of amisulpride was 48.15-2,407.59 ng/ml, while the extraction recovery was 98.7-101.3%. The strategy established in the study, which was successfully applied to therapeutic drug monitoring of amisulpride for routine clinical detection, displays high sensitivity, good repeatability, convenience and low cost.

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Online Column Extraction Coupled with Double-Trap Column System for HPLC Determination of Valproic Acid in Human Plasma Without Derivatization

Peng

Pan

et al. 2021

Chromatographia

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A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

Cited by 8 publications

References 30 publications

A novel method for the quantification of anlotinib in human plasma using two‐dimensional liquid chromatography

A novel method for the quantification of anlotinib in human plasma using two‐dimensional liquid chromatography

A novel and sensitive method for determination of amisulpride in human plasma by two‐dimensional liquid chromatography

Online Column Extraction Coupled with Double-Trap Column System for HPLC Determination of Valproic Acid in Human Plasma Without Derivatization

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