A novel and sensitive method for determination of amisulpride in human plasma by two‐dimensional liquid chromatography

Abstract

A novel and sensitive heart-cutting two-dimensional liquid chromatography with ultraviolet detection method (2D-LC-UV) was developed and validated for determination of amisulpride in human plasma. The 2D-LC system consists of a first dimensional ( 1 D) LC column and a middle transfer column as well as a second-dimensional ( 2 D) LC column. After simple protein precipitation, the sample was directly injected into the introduction valve of the 2D-LC system. The 1 D column, playing a role of primary separation and preconcentration for complex plasma matrices, transferred the targets to the intermediate column. Following capture of targets on the middle column online, the analytes were transferred to the 2 D separation column by a six-port valve. The 2 D column, avoiding interference from the plasma matrix, completed further separation and quantification. An assistant pump was optimized for primary enrichment as well as final elution in the heart-cutting mode. The analytical time of amisulpride was 7.401 min. The accuracy was between 0.48 and 8.49%, while the intra-and inter-day precisions ranged from 0.9 to 3.1% and from 1.7% to 3.3%, respectively. The linear range of amisulpride was 48.15-2,407.59 ng/ml, while the extraction recovery was 98.7-101.3%. The strategy established in the study, which was successfully applied to therapeutic drug monitoring of amisulpride for routine clinical detection, displays high sensitivity, good repeatability, convenience and low cost.

The eGFR parameter model helps predict blood drug concentration of amisulpride in patients with schizophrenia

“…After centrifuging at a high speed of 20000 rpm for 10 minutes, 1000 µL of supernatant was transferred into a 2 mL sampling bottle, 500 µL of which would be used for analysis. The 500 µL supernatant was analyzed using two-dimensional high-performance liquid chromatography with ultraviolet detection (2D-LC-UV) [13].…”

Metabolic Enzymes and Drug Concentration Detection for Psychiatry and Psychology Medicines

“…After centrifugation at 20,000 rpm for 8 min, 1000 μL of supernatant was transferred into a 2 mL sampling bottle, 500 μL of which was ready for analysis. The 500 μL supernatant was verified using two-dimensional high-performance liquid chromatography with ultraviolet detection (2D-LC-UV) [1]. The method Z score was satisfactory (0.04); the method was certified by the Laboratory of the Government Chemist in the UK.…”

Amisulpride steady-state plasma concentration and adverse reactions in patients with schizophrenia: a study based on therapeutic drug monitoring data

“…The amisulpride steady-state plasma concentration of the patients was determined by two-dimensional liquid chromatography. The method was developed and validated in our lab (Qu et al , 2021). Amisulpride in 400 μl plasma was extracted by the addition of acetonitrile.…”