A novel approach using metabolomics coupled with hematological and biochemical parameters to explain the enriching-blood effect and mechanism of unprocessed Angelica sinensis and its 4 kinds of processed products

Ji, Peng; Wei, Yanming; Hua, Yuejin; Zhang, Xiaosong; Yao, Wan-Ling; Yuan, Ziwen; Wen, Yeong-Ray; Yang, Chaoxue

doi:10.1016/j.jep.2017.09.028

Cited by 36 publications

(41 citation statements)

References 35 publications

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“…RBC, WBC, HGB, and HCT are the main diagnostic criteria for blood deficiency syndrome (Ashour, 2014). As Na + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase are related to blood cells, they are closely associated with anemia (Ji et al, 2018). In our work, pharmacological indicators such as weight, viscera indices, blood parameters, the content of Na + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase, and the recovery of potential metabolites all showed that APAM and AR had comparable blood enriching effects.…”

Section: Blood Enriching Effect Of Apam and Arsupporting

confidence: 61%

“…Valine can regulate blood sugar levels and provide an energy supply for the body. In blood deficiency, valine provides extra energy to the muscle for glucose production, which can prevent muscle weakness (Ji et al, 2018). Leucine and isoleucine are important ketogenic amino acids, they can be converted to the metabolytic intermediate product 3-hydroxybutyric acid (3-HB) for energy production (Shi, Xiao, Wang, & Tang, 2013).…”

Section: Valine Leucine and Isoleucine Biosynthesismentioning

confidence: 99%

“…Alanine is an important non-essential amino acid in the body and is a significant element of lymphocyte regeneration and immunologic processes (Newsholme, Bender, Kiely, & Brennan, 2007). Aspartic acid is an α-amino acid and used in the synthesis of threonine and methionine (Ji et al, 2018). Methionine can enhance the activity of glutathione peroxidase and superoxide dismutase to increase the biosynthesis of GSH (Ji, 2015), which acts as an important antioxidant in human body and has the functions of anti-oxidation, anti-apoptosis and inhibition of inflammatory reaction Lu, 2013).…”

Section: Alanine Aspartate and Glutamate Metabolismmentioning

confidence: 99%

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Comparison of aerial parts of Astragalus membranaceus and Astragali Radix based on chemical constituents and pharmacological effects

Wang

Lü

et al. 2019

Food and Agricultural Immunology

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This study aims to provide a scientific basis for the comprehensive utilization of the aerial parts of Astragalus membranaceus (APAM). UPLC-Qtrap HRMS was used to identify secondary metabolites in APAM and Astragali Radix (AR), the pharmacological effects of them in N-acetyl phenylhydrazine (APH) and cyclophosphamide (Cy)-induced mice were studied by using 1 H NMR-based metabolomics. The results showed that the secondary metabolites of APAM and AR were different, but that there was no difference in the contents of eight constituent flavonoids and four constituent saponins, which were 0.8800 and 4.4351 mg/g in APAM and 0.8944 and 4.6422 mg/g in AR, respectively. APAM and AR induced beneficial effects on the recovery of body weight, blood parameters, viscera indices, Na + -K + -ATPase, and Ca 2+ -Mg 2 + -ATPase, the blood-enriching mechanisms of them were probably related to valine, leucine, and isoleucine biosynthesis, D-glutamine and D-glutamate metabolism, alanine, aspartate, and glutamate metabolism, and taurine and hypotaurine metabolism. ARTICLE HISTORY

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Section: Blood Enriching Effect Of Apam and Arsupporting

confidence: 61%

Section: Valine Leucine and Isoleucine Biosynthesismentioning

confidence: 99%

Section: Alanine Aspartate and Glutamate Metabolismmentioning

confidence: 99%

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Comparison of aerial parts of Astragalus membranaceus and Astragali Radix based on chemical constituents and pharmacological effects

Wang

Lü

et al. 2019

Food and Agricultural Immunology

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“…Studies have shown that proper processing of Chinese herbal medicines before clinical use can not only change their pharmacological activities and reduce their side effects, but also increase the curative effect (Singhuber, Zhu, Prinz, & Kopp, ; Zhao et al, ). Our previous studies showed that the blood‐enriching effect of JDG (Ji et al, ), the anti‐inflammatory effect of volatile oil from JDG or CDG and the hepatoprotective effect of polysaccharides from CDG were significantly enhanced (Hua, Xue, Zhang, Wei, & Ji, ; Zhong et al, ), which may be caused by changes in its chemical composition and content (Ji et al, ). At present, research on the herb pair Gui‐Hong and Danggui‐zhiqiao has shown a significant effect on promoting blood circulation and relieving blood stasis (Li et al, ; Wang, Li, et al, ).…”

Section: Introductionmentioning

confidence: 99%

Metabolomics study on promoting blood circulation and ameliorating blood stasis: Investigating the mechanism of Angelica sinensis and its processed products

Yuan

Zhong

Hua

et al. 2019

Biomedical Chromatography

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Angelica sinensis (Danggui, DG) parched with alcohol (Jiu Danggui, JDG) and charred DG are the main processed products of DG, which are used to treat blood stasis syndrome (BSS). However, their therapeutic effect and mechanisms are still unclear. Based on an acute rat BSS model, the intervention effects of DG and its processed products (DGPPs) were evaluated by the hemorheology and coagulation function parameters. Meanwhile, plasma and urine metabolites were detected and analyzed by liquid chromatography coupled to quadrupole time‐of‐flight mass spectrometry and multivariate statistical analysis method. The results of hemorheology, coagulation function parameters and metabolomics all showed that the BSS model was successfully established, DGPPs intervention could significantly relieve rats BSS and the therapeutic effect of JDG was best. Moreover, 23 differential metabolites (14 in plasma and nine in urine) were identified that were closely related to the BSS, involving seven potential target metabolic pathways. DGPP intervention showed different degrees of reverse effect on these metabolites. JDG was the most effective owing to extensive regulation effect on differential metabolites. This study provides a reference for understanding the pathological mechanism of BSS and the mechanism of DGPPs, which lays a theoretical foundation for the rational use of DGPPs in clinical practice.

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“…For biomarker search, PLS-DA loadings were sorted by absolute values, being the first ones the metabolites responsible of the separation between experimental groups. Because both PCA and PLS-DA analysis may be influenced by variable correlations, the intra- and the inter-class variance of metabolites may have no significant differences in the one-dimensional statistical analysis [17]. For this reason, spectral bins were also selected using a Volcano plot with paired student t test analysis between HS over TN cohorts to get a general overview of the data (log 2 fold change thresholds, ≤1.5 and ≥1.5; P<0.01).…”

Section: Statistical Analysesmentioning

confidence: 99%

Heat stress modifies the lactational performances and the urinary metabolomic profile related to gastrointestinal microbiota of dairy goats

Contreras-Jodar

Nayan

Hamzaoui

et al. 2018

Preprint

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22 23 ¶ These authors contributed equally to this work 24 25 26 27 2 28 Abstract29 The aim of the study was to identify the candidate biomarkers of heat stress (HS) in the 30 urine of lactating dairy goats through the application of proton Nuclear Magnetic 31 Resonance ( 1 H NMR)-based metabolomic analysis. Dairy does (n = 16) in mid-lactation 32 were submitted to thermal neutral (TN; indoors; 15 to 20°C; 40 to 45% humidity) or HS 33 (climatic chamber; 37°C day, 30°C night; 40% humidity) conditions according to a crossover 34 design (2 periods of 21 days). Thermophysiological traits and lactational performances 35 were recorded and milk composition analyzed during each period. Urine samples were 36 collected at day 15 of each period for 1 H NMR spectroscopy analysis. Principal component 37 analysis (PCA) and partial least square-discriminant analysis (PLS-DA) assessment with 38 cross validation were used to identify the goat urinary metabolome from Human 39 Metabolome Data Base. HS increased rectal temperature (1.2°C), respiratory rate (3.5-40 fold) and water intake (74%), but decreased feed intake (35%) and body weight (5%) of the 41 lactating does. No differences were detected in milk yield, but HS decreased the milk 42 contents of fat (9%), protein (16%) and lactose (5%). Metabolomics allowed separating TN 43 and HS urinary clusters by PLS-DA. Most discriminating metabolites were hippurate and 44 other phenylalanine (Phe) derivative compounds, which increased in HS vs. TN does. The 45 greater excretion of these gut-derived toxic compounds indicated that HS induced a 46 harmful gastrointestinal microbiota overgrowth, which should have sequestrated aromatic 47 amino acids for their metabolism and decreased the synthesis of neurotransmitters and 48 thyroid hormones, with negative impact on milk yield and composition. In conclusion, HS 49 markedly changed the thermophysiological traits and lactational performances of dairy 50 goats, which were translated into their urinary metabolomic profile through the presence 51 of gut-derived toxic compounds. Hippurate and other Phe-derivative compounds are 52 suggested as urinary biomarkers to detect heat stressed dairy animals in practice. 53 54 Introduction 55 Exposure to high ambient temperature induces several physiological responses in order to 56 maintain body homeostasis. Animals suffer from heat stress (HS) when physiological 57 mechanisms fail to counterbalance an excessive heat load [1]. Exposure of dairy animals to 58 HS results in a decline in their productive [2] and reproductive [3] performances due to a 59 strong metabolic disruption. Dairy animals under HS typically show decreased feed intake,60 increased water consumption and thermophysiological traits, such as respiratory rate and 61 rectal temperature, when compared to thermoneutral (TN). Usually, HS reduces milk yield 62 and impairs milk composition in dairy goats [4]. Although these negative effects on milk 3 63 production are traditionally attributed to a decline in feed intake, pair-fed TN experiments ...

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A novel approach using metabolomics coupled with hematological and biochemical parameters to explain the enriching-blood effect and mechanism of unprocessed Angelica sinensis and its 4 kinds of processed products

Cited by 36 publications

References 35 publications

Comparison of aerial parts of Astragalus membranaceus and Astragali Radix based on chemical constituents and pharmacological effects

Comparison of aerial parts of Astragalus membranaceus and Astragali Radix based on chemical constituents and pharmacological effects

Metabolomics study on promoting blood circulation and ameliorating blood stasis: Investigating the mechanism of Angelica sinensis and its processed products

Heat stress modifies the lactational performances and the urinary metabolomic profile related to gastrointestinal microbiota of dairy goats

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