A novel aqueous micellar two-phase system composed of surfactant and mannitol for purification of polygalacturonase enzyme from<i>Durio zibethinus Murray</i>and recycling phase components

Amid, Mehrnoush; Asmadi, Farhana Azmira; Hussin, Muhaini; Manap, Mohd Yazid Abdul; Sarker, Md. Zaidul Islam; Chong, Gun Hean

doi:10.1080/01496395.2016.1142562

Cited by 3 publications

(1 citation statement)

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“…Each collected flower-pedicel structure consisted of an approximately 1 mm region of the pedicel and 1.5 mm of the flower. The CE and PG were extracted according to the methods described by Awad and Lewis (1980) and Amid et al (2016) . The enzyme activity was assayed in AZs at the same five developmental stages used for BTS and histological analyses, following the manuscript’s protocol of plant CE ELISA kit and plant PG ELISA kit (Shanghai Enzyme-linked Biotechnology Co., Ltd., China), respectively.…”

Section: Methodsmentioning

confidence: 99%

Histological Characteristics, Cell Wall Hydrolytic Enzymes Activity and Candidate Genes Expression Associated with Seed Shattering of Elymus sibiricus Accessions

et al. 2017

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Elymus sibiricus (siberian wildrye) is a perennial, cool-season, self-pollinating, and allotetraploid grass. As an economically important species, it has been widely grown and used for pasture and hay in northern China. Because of serious seed shattering (SS), however, E. sibiricus is difficult to grow for commercial seed production. To better understand the underlying mechanism of SS, we investigated the differences in SS of cultivars and wild accessions in relation to morphological and genetic diversity, histological characteristics, lignin staining, cell wall hydrolytic enzymes activity and candidate genes expressions. We found high level of morphological and genetic diversity among E. sibiricus accessions. In general, cultivars had higher average pedicel breaking tensile strength (BTS) value than wild accessions, of which PI655199 had the highest average BTS value (144.51 gf) and LQ04 had the lowest average BTS value (47.17 gf) during seed development. SS showed a significant correlation with seed length, awn length and 1000-seed weight. SS was caused by degradation of abscission layers that formed at early heading stage, and degradation of abscission layers occurred at 14 days after heading. Histological analysis of abscission zone (AZ) showed a smooth fracture surface on the rachilla in high SS genotype, suggesting higher degradation degree of abscission layers. This may resulted from the increased cellulase and polygalacturonase activity found in AZ at seed physiological maturity. Staining of pedicels of two contrasting genotypes suggested more lignin deposition in low SS genotype may play a role in resistance of SS. Furthermore, candidate genes that involved in cell wall-degrading enzyme and lignin biosynthesis were differentially expressed in AZ, indicating the involvement and role in SS. This study provided novel insights into the mechanism of SS in E. sibiricus.

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Section: Methodsmentioning

confidence: 99%