A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples

Ciuti, Riccardo; Liguri, Gianfranco

doi:10.4236/jbise.2017.102007

Cited by 8 publications

(7 citation statements)

References 37 publications

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“…The TAC assay measures the overall contribution of all the antioxidants present in the sample to its redox balance (Ciuti and Liguri, 2017). The significant depression of TAC levels observed in control group is in agreement with Pivoto et al (2015) and may be attributed to oxidative stress developed by the helminth parasites.…”

Section: Total Antioxidant Capacitysupporting

confidence: 56%

“…The hemolysate was used for the estimation of Total Antioxidant Capacity (TAC) and activities of superoxide dismutase (SOD) and catalase. Since glutathione is the most concentrated intracellular species that plays a crucial role in protection from free radicals, TAC was assayed according to the method of Ciuti and Liguri (2017) and expressed in terms of mM of reduced glutathione equivalents per gram of haemoglobin. The results were normalized with respect to sample's haemoglobin.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Adhatoda vasica (Acanthaceae) on Antioxidant and Immunological Profile in Sheep

Joy¹,

Prasad²,

Padmaja³

et al. 2022

IJAR

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Background: A study was conducted to evaluate the antioxidant and immunological profile of methanolic extract of Adhatoda vasica leaves (AVE) in naturally infected sheep with gastrointestinal nematodes and compared with commonly used anthelmintic albendazole in sheep. Methods: Fifty four sheep found positive for gastrointestinal nematodes were divided into 3 equal groups, GI (infected-untreated: control), GII (infected- AVE treated) and GIII (infected- albendazole treated). Blood samples were drawn on 5th and 14th day after treatment for analysis of oxidative stress markers such as TBARS, TAC, SOD and catalase and immunological parameters such as total immunoglobulin, IgG and IL-10. Result: Helminthiasis in sheep cause a considerable oxidative stress. The anthelmintic activity of Adhatoda vasica surpassed the conventional synthetic drug albendazole in sheep in terms of oxidative and immunological changes in blood.

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Section: Total Antioxidant Capacitysupporting

confidence: 56%

Section: Methodsmentioning

confidence: 99%

Effect of Adhatoda vasica (Acanthaceae) on Antioxidant and Immunological Profile in Sheep

Joy¹,

Prasad²,

Padmaja³

et al. 2022

IJAR

View full text Add to dashboard Cite

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“…The determination of total antioxidant capacity (TAC) in serum samples of the validation cohort, was performed as previously described (Ciuti and Liguri, 2017). Briefly, serum samples and glutathione (used as calibrator) were mixed 1:5 (v/v) with a pre-heated chromogenic reagent as described (Ciuti and Liguri, 2017) in a multi-well plate. Absorbance at 630 nm was determined in a UT2100C microplate reader (MRC, Holon, Israel) and measurements were taken at 20 and 120 s after the last reagent dispensing.…”

Section: Methodsmentioning

confidence: 99%

“…Absorbance at 630 nm was determined in a UT2100C microplate reader (MRC, Holon, Israel) and measurements were taken at 20 and 120 s after the last reagent dispensing. TAC quantification was performed as previously described (Ciuti and Liguri, 2017).…”

Section: Methodsmentioning

confidence: 99%

Unraveling the Serum Metabolomic Profile of Post-partum Depression

et al. 2019

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Post-partum depression (PPD) is a severe psychiatric disorder affecting ∼15% of young mothers. Early life stressful conditions in periconceptual, fetal and early infant periods or exposure to maternal psychiatric disorders, have been linked to adverse childhood outcomes interfering with physiological, cognitive and emotional development. The molecular mechanisms of PPD are not yet fully understood. Unraveling the molecular underpinnings of PPD will allow timely detection and establishment of effective therapeutic approaches. To investigate the underlying molecular correlates of PPD in peripheral material, we compared the serum metabolomes of an in detail characterized group of mothers suffering from PPD and a control group of mothers, all from Heraklion, Crete in Greece. Serum samples were analyzed by a mass spectrometry platform for targeted metabolomics, based on selected reaction monitoring (SRM), which measures the levels of up to 300 metabolites. In the PPD group, we observed increased levels of glutathione-disulfide, adenylosuccinate, and ATP, which associate with oxidative stress, nucleotide biosynthesis and energy production pathways. We also followed up the metabolomic findings in a validation cohort of PPD mothers and controls. To the very best of our knowledge, this is the first metabolomic serum analysis in PPD. Our data show that molecular changes related to PPD are detectable in peripheral material, thus paving the way for additional studies in order to shed light on the molecular correlates of PPD.

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“…The evaluation of salivary composition may be a practical, safe, easy, cheap, non-invasive, and rapid method that would be especially suitable for children. It is known that the overall ability of salivary samples to reduce a redox chromogen can be measured by a quantitative biochemical method and normalized by the concentration of salivary proteins [ 13 ]. Thus, the aim of this study was to quantify the variation of saliva, protein concentration, and antioxidant capacity in adult subjects with OSAS (OG) compared with a control group of healthy subjects (CG).…”

Section: Introductionmentioning

confidence: 99%

Saliva Analysis of pH and Antioxidant Capacity in Adult Obstructive Sleep Apnea Patients

Venza

Alloisio

Gioia

et al. 2022

IJERPH

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Background: Obstructive sleep apnea syndrome (OSAS) may be associated with and activates the stress response system, and variation in the physiological antioxidant capacity of body fluids. Our aim was to evaluate the variation of pH and antioxidant capacity on the saliva of obstructive sleep apnea subjects (OG) compared to a control group (CG). Method: Fifty subjects with moderate/severe OSAS were recruited in Tor Vergata Hospital and compared with 20 healthy subjects CG. The buffer and the antioxidant capacity of the samples were quantified measuring the pH and the percentage of total salivary antioxidant capacity (%TAC), which refers to the reduced glutathione salivary concentration (GSH). Moreover, the protein concentration and the gelatinolytic activity of metalloproteinases were quantified. Results: The data showed that the pH value is slightly more alkaline in OSAS subjects; however, it is not directly related to the severity of OSAS. The %TAC was found to be significantly reduced by 86.2% in the OG. Proteins of saliva from the OG were found 1.5 times more concentrated than in the healthy sample. The gelatinolytic activity of metalloproteinases of healthy and OSA did not show statistically significant changes. Conclusions: The salivary samples from OSAS compared to CG show an alteration of the oxidative state, the pH buffering power, and protein concentrations, inducing conditions that can easily evolve chronic gingivitis. Further investigations are necessary to evaluate the feasibility of using salivary fluid for the early diagnosis of oral or systemic problems in OSAS subjects.

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A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples

Cited by 8 publications

References 37 publications

Effect of Adhatoda vasica (Acanthaceae) on Antioxidant and Immunological Profile in Sheep

Effect of Adhatoda vasica (Acanthaceae) on Antioxidant and Immunological Profile in Sheep

Unraveling the Serum Metabolomic Profile of Post-partum Depression

Saliva Analysis of pH and Antioxidant Capacity in Adult Obstructive Sleep Apnea Patients

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