A novel assay for the evaluation of the prooxidant–antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients

Alamdari, Daryoush Hamidi; Paletas, Konstantinos; Pegiou, Theodosia; Sarigianni, Maria; Befani, Christina; Koliakos, George

doi:10.1016/j.clinbiochem.2006.10.017

Cited by 197 publications

(169 citation statements)

References 32 publications

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“…Furthermore, some previous studies have shown that the lipid profile changes during pregnancy and this may cause endothelial dysfunction that may play a role in generating oxidative stress. Also, Alamdari et al (18) have shown that there may be a relationship between PAB and the lipid profile.…”

Section: Introductionmentioning

confidence: 99%

“…We have recently applied a simple, rapid and inexpensive method that can measure the balance of oxidants and antioxidants simultaneously in one assay (the PAB assay) that uses the TMB-TMB cation and provides a redox index (18), and has been validated (19). Furthermore, some previous studies have shown that the lipid profile changes during pregnancy and this may cause endothelial dysfunction that may play a role in generating oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Prooxidant–Antioxidant Balance during Uncomplicated Pregnancy Using a Rapid Assay

Boskabadi¹,

Moeini²,

Tara³

et al. 2013

Journal of Medical Biochemistry

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SummaryBackground: Oxidative stress is thought to be a major contributor to complications during pregnancy, for example preeclampsia. However, reports regarding prooxidant-antioxidant balance in uncomplicated pregnancy are inconsistent. In this study, we aimed to compare the levels of oxidative stress in non-pregnant women with apparently normal pregnant women during the first trimester and at delivery. Methods: An assay for the determination of prooxidantantioxidant balance (PAB) was used in this study, in which the prooxidant burden and the antioxidant capacity were measured simultaneously in a single assay. The levels of oxidative stress were determined in 85 non-pregnant and 64 primigravid pregnant women. Results: Demographic data and biochemical indices did not differ significantly between the groups. Differences between PAB values were significant based on one-way ANOVA analysis (P<0.001). Using a post hoc test, we observed a statistically significant increase in PAB values during the first trimester and last trimester (P<0.001). Conclusions: Normal pregnancy is associated with a change in the measure of redox status, as assessed by the PAB assay.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of Prooxidant–Antioxidant Balance during Uncomplicated Pregnancy Using a Rapid Assay

Boskabadi¹,

Moeini²,

Tara³

et al. 2013

Journal of Medical Biochemistry

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“…In the present study, we applied a recently reported method (the PAB assay) 14,15 for estimating OS before and after a hemodialysis session for patients undergoing regular hemodialysis. In previous evaluation studies, the assay showed a linear response against all major serum oxidants and antioxidants.…”

Section: Discussionmentioning

confidence: 99%

“…In previous evaluation studies, the assay showed a linear response against all major serum oxidants and antioxidants. 14,15 In addition, the PAB assay has been previously compared to most of the widely utilized and documented methods that aim to estimate OS and permanent oxidative damage. 14 A signifi cant increase of OS before dialysis was observed in HD patients in comparison with healthy volunteers.…”

Section: Discussionmentioning

confidence: 99%

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Oxidative Stress Modulation Immediately After Hemodialysis

Alamdari

Honarmand

Sarrafnejad

et al. 2009

Dialysis & Transplantation

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AIMThe aim of this study was to estimate oxidative stress (OS) before and after dialysis in hemodialysis (HD) patients and correlate this stress with routine biochemical parameters.METHODSera of patients (n = 21), under regular HD, were collected 5 minutes before and after an HD session. Oxidative stress was estimated using the pro‐oxidant‐antioxidant assay (PAB), along with routine biochemical parameters in the same sera.RESULTSA significant increase of OS value was observed in HD patients 5 minutes after dialysis. Before HD, a significant correlation was established between the PAB values and fasting blood sugar, calcium, and C‐reactive protein (CRP); and an inverse correlation between the PAB values and uric acid, serum creatinine, albumin, and albumin/globulin ratio. This correlation was not obvious after HD. PAB value, after dialysis, correlated significantly only with serum iron and inversely with alanine transaminase.CONCLUSIONThis study found that the already‐increased OS in HD patients is further increased immediately after dialysis. However, factors influencing post‐dialysis OS may be different from pre‐dialysis factors.

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Age and sex‐specific reference intervals for prooxidant‐antioxidant balance, anti‐heat‐shock protein 27 (anti‐hsp27), and routine laboratory tests in the middle‐aged adult population

Ghazizadeh

Bohn

Yaghooti‐Khorasani

et al. 2021

Biotech and App Biochem

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Introduction:We aimed to define specific reference intervals (RIs) for 11 biomarkers including inflammatory and oxidative stress biomarkers, liver, and renal function tests in a healthy Iranian adult population for the first time. Methods: CLSI Ep28-A3 guidelines were then used to calculate accurate ageand sex-as well as body mass index (BMI)-specific RIs. Results: RIs for studied biomarkers showed no significant age and sex-specific differences, except for uric acid, which had higher concentrations in men when compared to women. Additionally, after partitioning the participants based on the BMI with a cutoff point of 25 kg/m 2 , only the levels of hs-CRP were positively associated with higher BMI (RI for BMI>25: 0.

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A novel assay for the evaluation of the prooxidant–antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients

Cited by 197 publications

References 32 publications

Determination of Prooxidant–Antioxidant Balance during Uncomplicated Pregnancy Using a Rapid Assay

Determination of Prooxidant–Antioxidant Balance during Uncomplicated Pregnancy Using a Rapid Assay

Oxidative Stress Modulation Immediately After Hemodialysis

Age and sex‐specific reference intervals for prooxidant‐antioxidant balance, anti‐heat‐shock protein 27 (anti‐hsp27), and routine laboratory tests in the middle‐aged adult population

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