A novel automated method for measuring the effect of analgesics on formalin-evoked licking behavior in rats

Sakiyama, Yojiro; Sujaku, Tetsujo; Furuta, Akihiro

doi:10.1016/j.jneumeth.2007.08.003

Cited by 15 publications

(8 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Injection of formalin (2.5%, 50 μL) into the ventral surface of the right hind paw evoked a characteristic biphasic nociceptive response in animal models as previously reported (13). It consisted of an initial intense response to pain beginning immediately after formalin injection and rapidly decaying within 10 min after formalin injection (first/ neurogenic phase) and then followed by a slowly rising but longer lasting response (second/inflammatory phase) from 10 to 60 mins after formalin injection (14).…”

Section: Resultssupporting

confidence: 65%

Antinociceptive Effect of Hydroalcoholic Extract of Iranian Green tea in the Formalin Test in Rats

Arzi

Ghorbanzadeh²,

Khorasgani³

2013

Jundishapur J Nat Pharm Prod

View full text Add to dashboard Cite

Background: Tea (Camellia sinensis) has been utilised, since time immemorial, as a beverage possessing encouraging health benefits. Little scientific evidence exists in literature on the effect of this plant on pain. Objectives: To investigate the antinociceptive activity of Iranian green tea extract. Materials and Methods:The hydroalcoholic extract was administered to male Wistar rats. Formalin paw test was used to evaluate the antinociceptive activity. Plant extract (25, 50, 100 and 200 mg/kg, i.p.) (n = 6 for each group) or vehicle (n = 6) was administered 30 min before the subplantar formalin injection. Results: The extract caused a significant dose-related (50, 100, 200 mg /kg, i.p.) inhibition of the first phase and onset of chronic phase (200 mg /kg, i.p.) of formalin induced nociception. The results showed that the pre-treatment of rats with naloxone (1 mg/kg, i.p.) significantly (P < 0.001) reversed antinociception by Green tea extract (GTE) (200 mg/kg, i.p.) in the inflammatory phase and had no effect on phase 1. Conclusions: These results indicate that GTE produces dose-related antinociception in chemical pain model and one of its possible mechanisms involves opioid pathways.

show abstract

Section: Resultssupporting

confidence: 65%

Antinociceptive Effect of Hydroalcoholic Extract of Iranian Green tea in the Formalin Test in Rats

Arzi

Ghorbanzadeh²,

Khorasgani³

2013

Jundishapur J Nat Pharm Prod

View full text Add to dashboard Cite

show abstract

“…This non-specific pain evaluating method is suitable to detect the effects showed by weak analgesics. Pain is generated by endogenous inflammatory mediators such as serotonin and bradykinin which stimulate peripheral nociceptive neurons (Sakiyama et al, 2008 ). The pain-sensation in acetic acid induced model is triggered by localized inflammatory response for the release of free arachidonic acid from tissue phospholipids via cyclooxygenase (COX-1 and COX-2), and prostaglandin specifically PGE2 and PGF2 biosynthesis, the level of lipoxygenase products may also be increased in peritoneal fluids (Adzu et al, 2003 ).…”

Section: Discussionmentioning

confidence: 99%

Antinociceptive and Anxiolytic and Sedative Effects of Methanol Extract of Anisomeles indica: An Experimental Assessment in Mice and Computer Aided Models

Uddin¹,

Reza²,

Abdullah-Al-Mamun³

et al. 2018

Front. Pharmacol.

View full text Add to dashboard Cite

Anisomeles indica (L.) kuntze is widely used in folk medicine against various disorders including allergy, sores, inflammation, and fever. This research investigated the antinociceptive, anxiolytic and sedative effects of A. indica methanol extract. The antinociceptive activity was assessed with the acetic acid-induced writhing test and formalin-induced flicking test while sedative effects with open field and hole cross tests and anxiolytic effects with elevated plus maze (EPM) and thiopental-induced sleeping time tests were assayed. Computer aided (pass prediction, docking) analyses were undertaken to find out the best-fit phytoconstituent of total 14 isolated compounds of this plant for aforesaid effects. Acetic acid treated mice taking different concentrations of extract (50, 100, and 200 mg/kg, intraperitoneal) displayed reduced the writhing number. In the formalin-induced test, extract minimized the paw licking time of mice during the first phase and the second phase significantly. The open field and hole-cross tests were noticed with a dose-dependent reduction of locomotor activity. The EPM test demonstrated an increase of time spent percentage in open arms. Methanol extract potentiated the effect of thiopental-induced hypnosis in lesser extent comparing with Diazepam. The results may account for the use of A. indica as an alternative treatment of antinociception and neuropharmacological abnormalities with further intensive studies. The compound, 3,4-dihydroxybenzoic acid was found to be most effective in computer aided models.

show abstract

“…[1819] This consisted of an initial intense response to pain beginning immediately after formalin injection and rapidly decaying within 10 min after formalin injection (first/neurogenic phase) and then followed by a slowly rising but longer lasting response (second/inflammatory phase) from 10 to 60 mins after formalin injection with the maximum effect at approximately 20–30 min after formalin injection. [2021]…”

Section: Resultsmentioning

confidence: 99%

Antinociceptive effect of an ethanolic extract of the aerial parts of Hilleria latifolia (Lam.) H. Walt. (Phytolaccaceae)

Woode

Abotsi

2011

J Pharm Bioall Sci

View full text Add to dashboard Cite

Background:Hilleria latifolia (Lam.) H. Walt. (Phytolaccaceae) is a perennial herb used in Ghanaian traditional medicine for the treatment of various painful conditions. Little scientific evidence exists in literature on the effect of this plant on pain.Materials and Methods:The present study examined the antinociceptive effect of the ethanolic extract of the aerial parts of H. latifolia in chemical (acetic acid-induced abdominal writhing, glutamate, formalin, and capsaicin tests) and thermal (tail immersion test) behavioral pain models in rodents. The possible mechanisms of the antinociceptive action were also assessed with various antagonists in the formalin test.Results:The H. latifolia extract (HLE) together with morphine and diclofenac (positive controls), showed significant antinociceptive activity in all the models used. The antinociceptive effect exhibited by HLE in the formalin test was partly or wholly reversed by the systemic administration of naloxone, theophylline, and atropine. Glibenclamide, ondansetron, yohimbine, nifedipine, and NG-L-nitro-arginine methyl ester (L-NAME), however, did not significantly block the antinociceptive effect of the extract. HLE, unlike morphine, did not induce tolerance to its antinociceptive effect in the formalin test after chronic administration; morphine tolerance did not also cross-generalize to HLE. Interestingly, also, the chronic concomitant administration of HLE and morphine significantly suppressed the development of morphine tolerance.Conclusion:Together, these results indicate that HLE produces dose-related antinociception in several models of chemical and thermal pain, without tolerance induction, through mechanisms that involve an interaction with adenosinergic, muscarinic cholinergic, and opioid pathways.

show abstract

A novel automated method for measuring the effect of analgesics on formalin-evoked licking behavior in rats

Cited by 15 publications

References 61 publications

Antinociceptive Effect of Hydroalcoholic Extract of Iranian Green tea in the Formalin Test in Rats

Antinociceptive Effect of Hydroalcoholic Extract of Iranian Green tea in the Formalin Test in Rats

Antinociceptive and Anxiolytic and Sedative Effects of Methanol Extract of Anisomeles indica: An Experimental Assessment in Mice and Computer Aided Models

Antinociceptive effect of an ethanolic extract of the aerial parts of Hilleria latifolia (Lam.) H. Walt. (Phytolaccaceae)

Contact Info

Product

Resources

About