A novel automated multi-cycle magnetic solid-phase extraction coupled to LC-MS/MS to study the disorders of six functional B vitamins in patients with gastroenterology and hyperhomocysteinemia

Gu, Yuting; Jiang, Fengli; Yuan, Xiangmei; Yu, Fan; Liang, Yan; Xiao, Can; Yang, Shuangshuang; Zhang, Meiwei; Ou, Meixian; Xu, Yang; Yu, Chen; Jia, Jingying; Li, Jie; Liu, Gangyi; Lu, Youli

doi:10.1016/j.jpba.2024.115989

Cited by 3 publications

(1 citation statement)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The one of fat-soluble vitamins contains retinol (VA), ercalcidiol (VD2), calcifediol monohydrate (VD3), and tocopherol (VE). The concentrations of vitamins in the standard solutions are determined according to those in normal human serum, but the values are slightly higher than the upper limits of the normal concentration ranges. , Table S2 summarizes the concentrations of 10 kinds of vitamins in their standard solutions. VE has the highest concentration of 54.6 μg·mL –1 .…”

Section: Resultsmentioning

confidence: 99%

Superlong Metal–Organic Framework Micro-/Nanofibers for Selective Vitamin Absorption

Xiong,

Lu,

Zhong

et al. 2024

Langmuir

Self Cite

View full text Add to dashboard Cite

Superlong MOF-74-type micro/nanofibers, which have aspect ratios much higher than 200, are synthesized via nanoparticulate MOF-mediated recrystallization. Co-MOF-74 microfibers have high crystallinity, whereas Co-MOF-74-II nanofibers are composed of nanocrystals and amorphous phases, even though they have nanofibrous morphology. Both MOFs consist of plenty of micropores with diameters in the range of 1.0 to 2.0 nm, and they exhibit high thermal stability with a decomposition temperature higher than 260.0 °C. The MOFs are demonstrated for selective absorption of some vitamins including riboflavin, folic acid, and 5-methyltetrahydrofolate. Co-MOF-74-II nanofibers can efficiently absorb riboflavin and folic acid from their aqueous solution with absorption percentages approaching 90.0%, and they have enhanced capability for absorbing tocopherol in methanol. The micro/nanofibrous morphology, together with the capability for selective vitamin absorption, makes the novel MOFs highly promising for applications in micro-solid-phase extraction.

show abstract

Section: Resultsmentioning

confidence: 99%

Superlong Metal–Organic Framework Micro-/Nanofibers for Selective Vitamin Absorption

Xiong,

Lu,

Zhong

et al. 2024

Langmuir

Self Cite

View full text Add to dashboard Cite

show abstract

Development and evaluation of a candidate reference measurement procedure for detecting 17α-hydroxyprogesterone in dried blood spots using isotope dilution liquid chromatography tandem mass spectrometry

He,

Dai,

Shen

et al. 2024

Anal Bioanal Chem

View full text Add to dashboard Cite

Abstract17α-Hydroxyprogesterone (17α-OHP) quantification in dried blood spots (DBS) is essential for newborn screening for congenital adrenal hyperplasia (CAH), which is challenging due to its low physiological concentration. The high false-positive rates of immunoassays necessitate the development of more accurate methods. Liquid chromatography tandem mass spectrometry (LC-MS/MS) offers increased specificity and sensitivity, yet standardized procedures for 17α-OHP measurement are required for clinical application. A candidate reference measurement procedure (cRMP) using isotope dilution LC-MS/MS was developed for 17α-OHP quantification in DBS. By utilizing stable isotope-labeled D8-17α-OHP as an internal standard, the cRMP was optimized, covering sample preparation, calibration, and LC-MS/MS analysis. The method performance was validated across several parameters, including precision, accuracy, specificity, detection limits, and matrix effects. Clinical applicability was further assessed through the establishment of reference intervals for healthy newborns. The developed cRMP exhibited a linear range of 1.00 to 80.00 ng/mL for 17α-OHP, with detection and quantification limits of 0.14 ng/mL and 0.52 ng/mL, respectively. Inter- and intraday precision demonstrated coefficients of variation within 1.27 to 5.69%. The recovery rates and matrix effects were well within acceptable limits, ensuring method reliability. Clinical application showed distinct reference intervals for healthy newborns that were unaffected by sex but influenced by weight and gestational age. This method significantly enhances CAH diagnostic accuracy in newborns, providing a valuable tool for clinical laboratories and improving newborn screening program standardization and traceability.

show abstract

Nanomaterial-based magnetic solid-phase extraction in pharmaceutical and biomedical analysis

Hou,

Hu,

et al. 2025

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

A novel automated multi-cycle magnetic solid-phase extraction coupled to LC-MS/MS to study the disorders of six functional B vitamins in patients with gastroenterology and hyperhomocysteinemia

Cited by 3 publications

References 32 publications

Superlong Metal–Organic Framework Micro-/Nanofibers for Selective Vitamin Absorption

Superlong Metal–Organic Framework Micro-/Nanofibers for Selective Vitamin Absorption

Development and evaluation of a candidate reference measurement procedure for detecting 17α-hydroxyprogesterone in dried blood spots using isotope dilution liquid chromatography tandem mass spectrometry

Nanomaterial-based magnetic solid-phase extraction in pharmaceutical and biomedical analysis

Contact Info

Product

Resources

About