A novel bioactive vitroceramic presents similar biological responses as autogenous bone grafts

Abstract

Bioactive glasses represent an interesting class of bone substitute's biomaterials. The present study investigated the repair of bone defects filled with a novel bioactive vitroceramic (Biosilicate(®)), alone or in association with particulate autogenous bone grafts in calvaria defects of rabbits. After 7, 14, and 30 days the specimens were retrieved for histological, histomorphometric and immunohistochemistry analysis. Satisfactory bone formation was observed in all groups, and direct bone-biomaterial surface was noted. Histomorphometric assessment did not show statistically significant differences in bone formation among the groups and periods, except for BG group at day 14. Immunoexpression of Runx-2 was similar among the groups containing the graft and the biomaterial, being more intense than in control group. Similar result was observed for VEGF expression, especially in the last experimental period. These results revealed that Biosilicate(®) presented a favorable behavior, comparable to autogenous bone graft.

Effects of Biosilicate® Scaffolds and Low-Level Laser Therapy on the Process of Bone Healing

“…According to previous studies, the quantity of positive cells per field was described by a scoring scale from 1 to 4 (1 = absent, 2 = weak, 3 = moderate, and 4 = intense). 19,20 The analysis was performed by two observers (C.T. and K.N.Z.…”

Evaluation of Crystallized Biosilicate in the Reconstruction of Calvarial Defects

“…Matsumoto et al [25] in a study using rabbit calvaria observed that Biosilicate associated with autogenous bone showed histometric and immunohistochemical results similar to the defects which were filled with nothing but autogenous bone. The microscopic analyses showed very similar neoformed tissue in both groups, suggesting that the degradation level of the biomaterial tends to be compatible to the bone formation.…”

Photobiomodulation and bone healing in diabetic rats: evaluation of bone response using a tibial defect experimental model

“…Additionally, internal positive controls were performed with each staining bath. RUNX2 and RANK-L immunoexpressions were evaluated both qualitatively (presence of the immunomarkers) and quantitatively in five predetermined fields using light microscopy (Leica Microsystems AG, Wetzlar, Germany) according to a previously described scoring scale from 1 to 4 (1=absent, 2=weak, 3=moderate, and 4=intense) for immunohistochemical analysis [20,21]. The analysis was performed by two observers in a blinded way.…”