A Novel Biocompatible Titanium–Gadolinium Quantum Dot as a Bacterial Detecting Agent with High Antibacterial Activity

Abstract

In this study, the titanium–gadolinium quantum dots (TGQDs) were novel, first of its type to be synthesized, and fully characterized to date. Multiple physical characterization includes scanning electron microscopy (SEM), scanning electrochemical microscope (SCEM), x-ray fluorescence, spectrophotometry, and dynamic light scattering were carried out. The obtained results confirmed appropriate size and shape distributions in addition to processing optical features with high quantum yield. The synthesized TGQD was used as a fluorescent dye for bacterial detection and imaging by fluorescent microscopy and spectrophotometry, where TGQD stained only bacterial cells, but not human cells. The significant antibacterial activities of the TGQDs were found against a highly pathogenic bacterium (Staphylococcus aureus) and its antibiotic resistant strains (vancomycin and methicillin resistant Staphylococcus aureus) using growth curve analysis and determination of minimum inhibitory concentration (MIC) analysis. Live/dead cell imaging assay using phase-contrast microscope was performed for further confirmation of the antibacterial activity. Cell wall disruption and release of cell content was observed to be the prime mode of action with the reduction of cellular oxygen demand (OD).

Characterization and in vitro Analysis of Probiotic-Derived Peptides Against Multi Drug Resistance Bacterial Infections

“…The solutions with different concentration of antimicrobial agent were added to the microplate wells and mixed with the bacterial cultures (0.5 MacFarland with final dilution 1:100 using MH medium). Later the plate was used in Multiskan EX for 24 h and results were evaluated the next day after 24 h to obtain the growth curves ( Jelinkova et al, 2018a ; Mazumdar et al, 2020 ; Sur et al, 2020a ).…”

Surface functionalization of endotracheal tubes coated with laccase–gadolinium phosphate hybrid nanoparticles for antibiofilm activity and contrasting properties

“…11 Among various metal-based nanoparticles, gadolinium nanostructures display superior diagnostic and therapeutic potential. 12 Moreover, gadolinium-based nanostructures have demonstrated potent antibacterial activity against some pathogenic bacteria and their antibiotic-resistant variants. 12 They also exhibit a contrast enhancement effect due to high X-ray attenuation.…”

“…12 Moreover, gadolinium-based nanostructures have demonstrated potent antibacterial activity against some pathogenic bacteria and their antibiotic-resistant variants. 12 They also exhibit a contrast enhancement effect due to high X-ray attenuation. 13 Therefore, these nanostructures could be incorporated throughout the length of the tube to visualize the placement of the ETT during X-ray.…”

Specific Inhibition of VanZ-Mediated Resistance to Lipoglycopeptide Antibiotics

“…At the same time, it is crucial to preserve the ability to use clinically accepted antibiotics, especially so-called “last resort” antibiotics, which are used for treating multiple drug-resistant infectious agents. The development of new therapeutics that modulate existing resistance mechanisms may increase the lifetime of already accepted antibiotics and overcome the spread of resistance [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ].…”