A novel bionic magnetic SERS aptasensor for the ultrasensitive detection of Deoxynivalenol based on “dual antennae” nano-silver

Zhao, Xudong; Shen, Huijing; Huo, Bingyang; Wang, Yu; Gao, Zhixian

doi:10.1016/j.bios.2022.114383

Cited by 36 publications

(15 citation statements)

References 38 publications

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“…Labeled solid‐phase substrates: 4‐aminothiophenol (4‐ATP), 4‐MBA, rhodamine B (R6G), 4‐MPBA, and so on are often used as Raman signal molecules (Guselnikova et al., 2022; Zhang, Ge, et al., 2022; Zhao et al., 2022). Additionally, specific recognition molecules, such as antibodies, aptamers, and bacteriophages, are frequently required in labeled detection strategies to achieve selective recognition, targeted isolation, and signal amplification of target strains (Xue et al., 2022; Yang, Lee, et al., 2022).…”

Section: Sers‐active Nanostructuresmentioning

confidence: 99%

Advances in surface‐enhanced Raman spectroscopy technology for detection of foodborne pathogens

Zhu

Ali

Jiao

et al. 2023

Comp Rev Food Sci Food Safe

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Rapid control and prevention of diseases caused by foodborne pathogens is one of the existing food safety regulatory issues faced by various countries and has received wide attention from all sectors of society. The development of rapid and reliable detection methods for foodborne pathogens remains a hot research area for food safety and public health because of the limitations of complex steps, time-consuming, low sensitivity, or poor selectivity of commonly used methods.Surface-enhanced Raman spectroscopy (SERS), as a novel spectroscopic technique, has the advantages of high sensitivity, selectivity, rapid and nondestructive detection and has exhibited broad application prospects in the determination of pathogenic bacteria. In this study, the enhancement mechanisms of SERS are briefly introduced, then the characteristics and properties of liquid-phase, rigid solid-phase, and flexible solid-phase are categorized. Furthermore, a comprehensive review of the advances in label-free or label-based SERS strategies and SERS-compatible techniques for the detection of foodborne pathogens is provided, and the advantages and disadvantages of these methods are reviewed.Finally, the current challenges of SERS technology applied in practical applications are listed, and the possible development trends of SERS in the field of foodborne pathogens detection in the future are discussed.

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Section: Sers‐active Nanostructuresmentioning

confidence: 99%

Advances in surface‐enhanced Raman spectroscopy technology for detection of foodborne pathogens

Zhu

Ali

Jiao

et al. 2023

Comp Rev Food Sci Food Safe

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“…As DON is a main pollutant in wheat, a rapid and sensitive detection method for DON is essential to monitor mildew in cereals and avoid the adverse effects of DON on human and animal health. 4,12,13 Electrochemiluminescence (ECL) is an electrochemical detection technology that combines electrochemistry and chemiluminescence. 14 Because of its characteristics of high sensitivity and simple operation, ECL has attracted considerable attention in the field of biosensing.…”

Section: Introductionmentioning

confidence: 99%

“…In China, according to GB 2761-2017, the limit of DON in cereals and their products is 1000 μg/kg. As DON is a main pollutant in wheat, a rapid and sensitive detection method for DON is essential to monitor mildew in cereals and avoid the adverse effects of DON on human and animal health. ,, …”

Section: Introductionmentioning

confidence: 99%

Screening-Capture-Integrated Electrochemiluminescent Aptasensor Based on Mesoporous Silica Nanochannels for the Ultrasensitive Detection of Deoxynivalenol in Wheat

Wang

Wei

et al. 2023

J. Agric. Food Chem.

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To prevent the contamination of cereals by mycotoxins, establishing a sensitive and rapid method for the detection of mycotoxins is essential. In this study, a screening-capture-integrated electrochemiluminescence (ECL) aptasensor based on mesoporous silica films (MSFs) was successfully prepared for the ultrasensitive and highly selective detection of deoxynivalenol (DON) in wheat. The narrow nanochannels of MSFs can realize size screening, thereby eliminating the influence of macromolecular substances and providing a pure environment for the signal probe (tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy) 3 2+ )) to reach the indium tin oxide (ITO) conductive substrate, which significantly improves the anti-interference ability of the screening-captureintegrated ECL sensor. The aptamer (Apt) attached to the surface of the MSFs can specifically capture DON, and the resulting DON-Apt complex has a gated effect on the MSFs, triggering the inhibition of Ru(bpy) 3 2+ in the electrolyte from reaching the ITO surface. Therefore, the ECL intensity of the sensor decreased with increasing DON concentration to achieve a quantitative detection of DON. Under optimized conditions, the linear range of the screening-capture-integrated ECL aptasensor was 0.001−200 μg/kg, and the detection limit was as low as 5.27 × 10 −5 μg/kg (S/N = 3). In conclusion, this study developed a screening-captureintegrated ECL aptasensor that combines size screening and specific capture for the detection of DON in wheat, providing a new approach for the early detection of wheat mildew.

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“…However, expensive instruments and the demand for trained technicians limit the development of instrumental methods. In addition, high prices and the stringent conditions of transportation and storage limit the practical applicability of antibody-based methods [ 5 ]. Therefore, it is highly desirable to explore simple, low-cost and sensitive OTA detection methods.…”

Section: Introductionmentioning

confidence: 99%

An Alkyne-Mediated SERS Aptasensor for Anti-Interference Ochratoxin A Detection in Real Samples

Wang

Chen

et al. 2022

Foods

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Avoiding interference and realizing the precise detection of mycotoxins in complex food samples is still an urgent problem for surface-enhanced Raman spectroscopy (SERS) analysis technology. Herein, a highly sensitive and specific aptasensor was developed for the anti-interference detection of Ochratoxin A (OTA). In this aptasensor, 4-[(Trimethylsilyl) ethynyl] aniline was employed as an anti-interference Raman reporter to prove a sharp Raman peak (1998 cm−1) in silent region, which could avoid the interference of food bio-molecules in 600–1800 cm−1. 4-TEAE and OTA-aptamer were assembled on Au NPs to serve as anti-interference SERS probes. Meanwhile, Fe3O4 NPs, linked with complementary aptamer (cApts), were applied as capture probes. The specific binding of OTA to aptamer hindered the complementary binding of aptamer and cApt, which inhibited the binding of SERS probes and capture probes. Hence, the Raman responses at 1998 cm−1 were negatively correlated with the OTA level. Under the optimum condition, the aptasensor presented a linear response for OTA detection in the range of 0.1–40 nM, with low detection limits of 30 pM. In addition, the aptasensor was successfully applied to quantify OTA in soybean, grape and milk samples. Accordingly, this anti-interference aptasensor could perform specific, sensitive and precise detection of OTA in real samples, and proved a reliable reference strategy for other small-molecules detection in food samples.

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A novel bionic magnetic SERS aptasensor for the ultrasensitive detection of Deoxynivalenol based on “dual antennae” nano-silver

Cited by 36 publications

References 38 publications

Advances in surface‐enhanced Raman spectroscopy technology for detection of foodborne pathogens

Advances in surface‐enhanced Raman spectroscopy technology for detection of foodborne pathogens

Screening-Capture-Integrated Electrochemiluminescent Aptasensor Based on Mesoporous Silica Nanochannels for the Ultrasensitive Detection of Deoxynivalenol in Wheat

An Alkyne-Mediated SERS Aptasensor for Anti-Interference Ochratoxin A Detection in Real Samples

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