2016
DOI: 10.1016/j.ab.2016.05.009
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A novel cell-based duplex high-throughput screening assay combining fluorescent Ca2+ measurement with homogeneous time-resolved fluorescence technology

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Cited by 2 publications
(2 citation statements)
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“…This may involve using calcium-sensitive dyes that visualize intracellular Ca 2+ mobilization. [112,113] An important consideration is that cell-based assays may not be able to define whether the effect results from the direct interaction between hits and targets or from hits indirectly interacting with other intracellular modulators of targets due to the complex intracellular environment. Although some drugs identified from cell-based screens do not have well-defined targets even after being approved, such as verapamil and diltiazem, research about their mechanisms of action (also called target deconvolution) will continue since it helps to decode the pathogenesis of a specific disease and direct the discovery or design of the next-generation of drugs.…”
Section: Cell-based Assaysmentioning
confidence: 99%
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“…This may involve using calcium-sensitive dyes that visualize intracellular Ca 2+ mobilization. [112,113] An important consideration is that cell-based assays may not be able to define whether the effect results from the direct interaction between hits and targets or from hits indirectly interacting with other intracellular modulators of targets due to the complex intracellular environment. Although some drugs identified from cell-based screens do not have well-defined targets even after being approved, such as verapamil and diltiazem, research about their mechanisms of action (also called target deconvolution) will continue since it helps to decode the pathogenesis of a specific disease and direct the discovery or design of the next-generation of drugs.…”
Section: Cell-based Assaysmentioning
confidence: 99%
“…This may involve using calcium‐sensitive dyes that visualize intracellular Ca 2+ mobilization. [ 112,113 ]…”
Section: Hts‐based Drug Discoverymentioning
confidence: 99%