A Novel Cell Delivery System Using Magnetically Labeled Mesenchymal Stem Cells and an External Magnetic Device for Clinical Cartilage Repair

Kobayashi, Takaaki; Ochi, Mitsuo; Yanada, Shinobu; Ishikawa, Masakazu; Adachi, Nobuo; Deie, Masataka; Arihiro, Koji

doi:10.1016/j.arthro.2007.08.017

Cited by 108 publications

(87 citation statements)

References 16 publications

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“…Previous studies have reported that cultured bone marrow stromal cells labeled with magnetic particles could be guided and gathered into a desired region by a magnetic force. 13,14 However, the present study is the first report of magnetic cell targeting of freshly isolated cells in a muscle injury model without requiring their in vitro culture. These findings have important implications, especially for the treatment of skeletal muscle injury.…”

Section: Discussionmentioning

confidence: 94%

“…In previous studies, the maximum magnetic flux density of the magnetic targeting system was *0.6 T. 13 However, since magnetic beads combined with CD133 antibodies are smaller than the magnetic particles (ferumoxides) used for the labeling of cells in the previous study, the flux magnetic density may need to be larger to control the transplanted CD133…”

Section: Discussionmentioning

confidence: 99%

“…In our magnetic cell targeting system, labeled cells can be guided into the desired region by a magnetic force from the extracorporeal device. 13,14 The CD133 + cells were labeled with a magnetic bead, and isolated from human peripheral blood using a magnetic cell separation instrument. 15 We hypothesized that immunomagnetic CD133…”

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confidence: 99%

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Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Ohkawa

Kamei²,

Kamei³

et al. 2013

Tissue Engineering Part C: Methods

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Skeletal muscle injuries often leave lasting functional damage or pain. Muscle injuries are routinely treated conservatively, but the most effective treatment to promote the repair of injured muscles has not yet been established. Our previous report demonstrated that human peripheral blood-derived CD133+ cell transplantation to rat skeletal muscle injury models inhibited fibrosis and enhanced myogenesis after injury. However, the acquisition of a sufficient number of cells remains the limitation for clinical application, as the CD133 + population is rare in human blood. In this study, we applied a magnetic cell targeting system to accumulate transplanted cells in the muscle injury site and to enhance the regenerative effects of CD133 + cell transplantation, focusing on the fact that CD133 + cells are labeled with a magnetic bead for isolation. For the magnetic cell targeting, the magnet field generator was set up to adjust the peak of the magnetic gradient to the injury site of the tibialis anterior muscle, and 1 · 10 4 human peripheral blood CD133 + cells were locally injected into the injury site. This cell number is 10% of that used in the previous study. In another group, the same number of CD133 + cells was injected without magnetic force. The CD133 + cells transplanted with the magnetic force were more accumulated in the muscle injury site compared with the CD133 + cells transplanted without the magnetic force. In addition, the transplantation of CD133+ cells under the magnetic control inhibited fibrous scar formation and promoted angiogenesis and myogenesis, and also upregulated the mRNA expression of myogenic transcription factors, including Pax7, MyoD1 and Myogenin. However, the transplantation of CD133 + cells without the magnetic force failed to demonstrate these effects. Thus, our magnetic cell targeting system enables transplantation of a limited number of CD133 + cells to promote the repair of skeletal muscle injury.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Ohkawa

Kamei²,

Kamei³

et al. 2013

Tissue Engineering Part C: Methods

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“…9,27-41 Kobayashi et al 38 reported that an external magnetic force could deliver magnetically labeled MSCs to a desired place; in that study, the authors injected magnetically labeled MSCs into swine knees. They demonstrated an ability to control the direction of magnetically labeled MSCs using an external magnetic device for arthroscopic treatment.…”

Section: Discussionmentioning

confidence: 99%

Articular cartilage repair using an intra‐articular magnet and synovium‐derived cells

Hori

Deie

Kobayashi

et al. 2010

Journal Orthopaedic Research

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The purpose of this study was to investigate the chondrogenic potential of magnetically labeled synovium-derived cells (M-SDCs) and examine whether M-SDCs could repair the articular cartilage using an intra-articular magnet after delivery to the lesion. Synovium-derived cells (SDCs) were cultured from the synovium of a rat knee, and were magnetically labeled with ferumoxides. M-SDCs were examined with a transmission electron microscope. A pellet culture system was used to evaluate the chondrogenic potential of M-SDCs in a magnetic field. In a rat model, allogeneic M-SDCs were injected into the knee after we made an osteochondral defect on the patellar groove and implanted an intra-articular magnet at the bottom of the defect. We histologically examined the defects at 48 h, 4 weeks, 8 weeks, and 12 weeks after treatment. Electron microscopy showed the transfection of ferumoxides into SDCs. The pellet cultures revealed the chondrogenic potential of M-SDCs in a magnetic field. M-SDCs accumulated in the osteochondral defect at 48 h after treatment, and we confirmed the regeneration of the articular cartilage at 4 weeks, 8 weeks, and 12 weeks after treatment using an intra-articular magnet. We demonstrated that articular cartilage defects could be repaired using an intra-articular magnet and M-SDCs. We believe that this system will be useful to repair human articular cartilage defects. Keywords: cell delivery system; magnet; synovium; intra-articular injection; cartilage repair Articular cartilage is well known to have very limited self-healing potential. There are many procedures, including drilling, abrasion, microfracture, and distraction, for repairing the articular cartilage.1-7 However, the defects are repaired by fibrous tissues during those procedures, 6,8 and a routine procedure for articular cartilage defect repair has not yet been established.Currently, engineered cartilage tissues are in widespread clinical use, and good clinical results have been achieved with these tissues.9,10 However, this procedure requires the harvesting of a small amount of cartilage tissues from the patient prior to cartilage transplantation. It is therefore necessary to implement a lessinvasive procedure.Recently, synovium-derived cells (SDCs) have been found to have a high potential for both proliferation and differentiation.11-15 Synovial tissues are easy to harvest and may be collected from any joint without damaging the articular cartilage. We therefore evaluated the method of transplanting SDCs for repairing articular cartilage defects.Previous studies have suggested that intra-articular injected mesenchymal stem cells (MSCs) mobilize to the injured tissues. 16 This transplantation was less invasive, but was inefficient because the injected cells spread throughout the joint space. Consequently, we created an intra-articular magnet which had a strong magnetic force, for efficient transplantation. The delivered cells were magnetically labeled with ferumoxides, which is approved by the United States Food and Drug Admi...

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“…Many authors have shown in animal and laboratory studies the use of mesenchymal stem cells with chondrogenic potential [9,10,26,28,59] but only few clinical studies have been done [11,12,58]. Once MSC are cultured in the appropriate microenvironment, they can differentiate to chondrocytes and form cartilage; onset of chondrogenesis requires a chemically defined serum-free medium supplemented with dexamethasone, ascorbic acid, and growth factors such as TGF-B [29] or other [7,8].…”

Section: One-step Surgery: Mesenchymal Stem Cellsmentioning

confidence: 99%

Second and Third Generation Cartilage Transplantation

2011

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A Novel Cell Delivery System Using Magnetically Labeled Mesenchymal Stem Cells and an External Magnetic Device for Clinical Cartilage Repair

Cited by 108 publications

References 16 publications

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Articular cartilage repair using an intra‐articular magnet and synovium‐derived cells

Second and Third Generation Cartilage Transplantation

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A Novel Cell Delivery System Using Magnetically Labeled Mesenchymal Stem Cells and an External Magnetic Device for Clinical Cartilage Repair

Cited by 108 publications

References 16 publications

Magnetic Targeting of Human Peripheral Blood CD133+Cells for Skeletal Muscle Regeneration

Magnetic Targeting of Human Peripheral Blood CD133+Cells for Skeletal Muscle Regeneration

Articular cartilage repair using an intra‐articular magnet and synovium‐derived cells

Second and Third Generation Cartilage Transplantation

Contact Info

Product

Resources

About

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration

Magnetic Targeting of Human Peripheral Blood CD133⁺Cells for Skeletal Muscle Regeneration