A novel cell disruption technique to enhance lipid extraction from microalgae

Steriti, Alberto; Rossi, Roberto; Concas, Alessandro; Cao, Giacomo

doi:10.1016/j.biortech.2014.04.056

Cited by 86 publications

(33 citation statements)

References 25 publications

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“…Several types of drying are applicable for algae: spray drying, drum drying, freeze-drying, belt drying and sun or wind drying, although it is stated that the latter is not very effective (Mata et al, 2010). Apart from these steps, cell disruption and extraction techniques can be used to release the metabolites that are of interest (Steriti et al, 2014;Pragya et al, 2013). Most technologies for algal growth, harvesting, and conversion are operational at a pilot scale (several squared meters or hectares).…”

Section: Algae As a Protein-rich Alternative For Soy Mealmentioning

confidence: 98%

Environmental sustainability analysis of a protein-rich livestock feed ingredient in The Netherlands: Microalgae production versus soybean import

Taelman

Meester

Dijk

et al. 2015

Resources, Conservation and Recycling

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Section: Algae As a Protein-rich Alternative For Soy Mealmentioning

confidence: 98%

Environmental sustainability analysis of a protein-rich livestock feed ingredient in The Netherlands: Microalgae production versus soybean import

Taelman

Meester

Dijk

et al. 2015

Resources, Conservation and Recycling

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“…One gram of sample was added to 9.53 mL of methanol and 0.47 mL of acetyl chloride to perform transesterification (Steriti et al, 2014). The mixture was then heated in a water bath at 333 K for 2 h. The mixture was subsequently cooled and opened.…”

Section: Transesterification Of Lipidsmentioning

confidence: 99%

Optimization of continuous lipid extraction from Chlorella vulgaris by CO 2 -expanded methanol for biodiesel production

Yang

Klinthong

Tan

2015

Bioresource Technology

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“…Like other microorganisms, yeasts are surrounded by rigid cell walls that have to be disrupted in order to obtain the valuable cell content . Generally, the yeast cell wall is composed by thick and rigid layer of complex carbohydrates and glycoproteins with high mechanical strength and chemical resistance . In consequence, the cell wall disruption is the most challenging part of lipid extraction process from yeast cells, since lipids are located inside the cell .…”

Section: Introductionmentioning

confidence: 99%

Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

Karim

Yousuf

Islam

et al. 2018

Biotechnology Progress

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The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min where the distance between electrodes was maintained at 2, 4, and 6 cm. Colony forming units (CFU) were counted to observe the cell viability under the high voltage electric field. The forces of the pulsing electric field caused significant damage to the cell wall of L. starkeyi and the disruption of microbial cells was visualized by field emission scanning electron microscopic (FESEM) image. After breaking the cell wall, lipid was extracted and measured to assess the efficiency of EP over other techniques. The extent of cell inactivation was up to 95% when the electrodes were placed at the distance of 2 cm, which provided high treatment intensity (36.7 kWh m ). At this condition, maximum lipid (63 mg g ) was extracted when the biomass was treated for 10 min. During the comparison, EP could extract 31.88% lipid while the amount was 11.89% for ultrasonic and 16.8% for Fenton's reagent. The results recommend that the EP is a promising technique for lowering the time and solvent usage for lipid extraction from microbial biomass. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:838-845, 2018.

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A novel cell disruption technique to enhance lipid extraction from microalgae

Cited by 86 publications

References 25 publications

Environmental sustainability analysis of a protein-rich livestock feed ingredient in The Netherlands: Microalgae production versus soybean import

Environmental sustainability analysis of a protein-rich livestock feed ingredient in The Netherlands: Microalgae production versus soybean import

Optimization of continuous lipid extraction from Chlorella vulgaris by CO 2 -expanded methanol for biodiesel production

Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

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