A Novel Cellulase Produced by a Newly Isolated Trichoderma virens

Abstract

Screening and obtaining a novel high activity cellulase and its producing microbe strain is the most important and essential way to improve the utilization of crop straw. In this paper, we devoted our efforts to isolating a novel microbe strain which could produce high activity cellulase. A novel strain Trichoderma virens ZY-01 was isolated from a cropland where straw is rich and decomposed, by using the soil dilution plate method with cellulose and Congo red. The strain has been licensed with a patent numbered ZL 201210295819.6. The cellulase activity in the cultivation broth could reach up to 7.4 IU/mL at a non-optimized fermentation condition with the newly isolated T. virens ZY-01. The cellulase was separated and purified from the T. virens culture broth through (NH4)2SO4 fractional precipitation, anion-exchange chromatography and gel filtration chromatography. With the separation process, the CMC specific activity increased from 0.88 IU/mg to 31.5 IU/mg with 35.8 purification fold and 47.04% yield. Furthermore, the enzymatic properties of the cellulase were investigated. The optimum temperature and pH is 50 °C and pH 5.0 and it has good thermal stability. Zn2+, Ca2+ and Mn2+ could remarkably promote the enzyme activity. Conversely, Cu2+ and Co2+ could inhibit the enzymatic activity. This work provides a new highly efficient T. virens strain for cellulase production and shows good prospects in practical application.

Isolation and Partial Characterisation of Thermophilic Cellulolytic Bacteria from North Malaysian Tropical Mangrove Soil

“…Lin et al . (2015) and Zeng et al . (2016) found out that the metal cations at 1 mM and 5 mM act as enhancer or activator of cellulase produced by B. subtilis YJ1 and T. virens .…”

Cloning a novel endo-1,4-β-d-glucanase gene from Trichoderma virens and heterologous expression in E. coli

“…201210295819.6) was used for extract the total mRNA. This strain was isolated by our laboratory, which was collected in China Center for Type Culture Collection (CCTCC) with numbered M2012205 (Zeng et al 2016). E. coli DH5α and E. coli BL21 (DE3) were respectively used for plasmid amplification and expression host cell.…”

“…However, discovery novel enzyme is always the eternal theme to enzyme research. In our previous work, we have isolated a novelty Trichoderma virens ZY-01, which can secrete high activity cellulase (Zeng et al 2016). Especially, the EG enzyme activity is very outstanding.…”

Production and Biochemical Characterization of Cellulase Enzyme by Trichoderma Strains from Harran Plain

“…Colonies similar to Trichoderma were selected and cultivated on PDA (Potato Dextrose Agar) under the same conditions to obtain single colonies [10].…”