A Novel Chitosan-γPGA Polyelectrolyte Complex Hydrogel Promotes Early New Bone Formation in the Alveolar Socket Following Tooth Extraction

Abstract

A novel chitosan-γPGA polyelectrolyte complex hydrogel (C-PGA) has been developed and proven to be an effective dressing for wound healing. The purpose of this study was to evaluate if C-PGA could promote new bone formation in the alveolar socket following tooth extraction. An animal model was proposed using radiography and histomorphology simultaneously to analyze the symmetrical sections of Wistar rats. The upper incisors of Wistar rats were extracted and the extraction sockets were randomly treated with gelatin sponge, neat chitosan, C-PGA, or received no treatment. The extraction sockets of selected rats from each group were evaluated at 1, 2, 4, or 6 wk post-extraction. The results of radiography and histopathology indicated that the extraction sockets treated with C-PGA exhibited lamellar bone formation (6.5%) as early as 2 wk after the extraction was performed. Moreover, the degree of new bone formation was significantly higher (P < 0.05) in the extraction sockets treated with C-PGA at 6 wk post-extraction than that in the other study groups. In this study, we demonstrated that the proposed animal model involving symmetrical sections and simultaneous radiography and histomorphology evaluation is feasible. We also conclude that the novel C-PGA has great potential for new bone formation in the alveolar socket following tooth extraction.

Human Fat-Derived Mesenchymal Stem Cells Xenogenically Implanted in a Rat Model Show Enhanced New Bone Formation in Maxillary Alveolar Tooth Defects

“…Animal procedures were performed in accordance with a protocol approved by the University of Tennessee, Institutional Animal Care and Use Committee (IACUC). Bone defects were generated using procedures modified from those described earlier [19][20][21]. Briefly, rats under anesthesia were placed in a supine position, and the mandible was opened to expose the maxillary surface.…”

Aplicação tópica de Bexident Gengivas® vs Bexident Post® – Efeitos na cicatrização pós extração. Estudo piloto

“…Alguns autores defendem a diminuição do tempo de cicatrização quando CHI associado à CHX, devido às características indutoras da angiogénese e da cicatrização, através da formação de tecido de granulação e re -epitelização, do CHI. 18,19,20 Novos estudos são necessários, com tempos de observação mais curtos (3, 7, 15 dias) e com uma componente de análise histológica (análise da presença de células inflamatórias, tecido de granulação, fibrina, fibras de colagénio); uma maior amostra; e maior seletividade nos dentes incluídos (divisão por grupos, pois o trauma a que os tecidos estão sujeitos é diferente). A existência de um único observador também deve ser adotado.…”

Polyelectrolyte complexes: mechanisms, critical experimental aspects, and applications

“…The biodegradable or biocompatible materials have been used as support matrices for delivery of bioactive species to target specific tissues and to promote three dimensional tissue reconstructions (Liu et al 2007). There are a number of reports explaining the potential of PECs in various areas of tissue engineering like bone tissue engineering (Coimbra et al 2011a), cartilage tissue engineering (Whu et al 2013), cardiac tissue engineering (Ceccaldi et al 2014), and dental tissue engineering (Chang et al 2014, Coimbra et al 2011b). developed PECs capable of MRI contrast enhancement using gadolinium (Gd).…”