A novel chloroplast localized Rab GTPase protein CPRabA5e is involved in stress, development, thylakoid biogenesis and vesicle transport in Arabidopsis

Karim, Sazzad; Alezzawi, Mohamed; Garcia-Petit, Christel; Solymosi, Katalin; Khan, Nadir Zaman; Lindquist, Emelie; Dahl, Peter; Hohmann, Stefan; Aronsson, Henrik

doi:10.1007/s11103-013-0161-x

Cited by 52 publications

(62 citation statements)

References 87 publications

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“…Arabidopsis CPRabA5e (where CP represents chloroplast) is a chloroplast-localized protein found to regulate oxidative stress signaling. The role of CPRabA5e was further validated by Y2H screen where 13 proteins previously predicted to be involved in stress and localized in thylakoids and plastoglobules were identifi ed (Karim et al 2014 ). However, the exact mechanism of CPRab5e activity is not known yet.…”

Section: Rab Gtpasesmentioning

confidence: 99%

Small and Large G Proteins in Biotic and Abiotic Stress Responses in Plants

Pandey

Sharma

Pandey

2015

Elucidation of Abiotic Stress Signaling in Plants

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The sessile nature of plants has enabled them to develop a higher level of plasticity in adaptation against the hostile environmental conditions such as biotic and abiotic stresses. The intricate molecular machineries in plants, consisting of signal sensors, signal transducers, and generation of response, are highly similar with animal's signal transduction components; however, a large number of pathways also show a high level of divergence. G proteins are a part of signal transduction components, which play pivotal role in regulating a large number of physiological and developmental processes in both plants and animals. G proteins or guanine nucleotide-binding proteins serve as molecular switches, existing in two forms: an active conformation, when bound to GTP, and an inactive conformation, when bound to GDP. In active conformation, G proteins interact with downstream effector molecules to initiate various cellular responses. Based on their structure, they have been divided into heterotrimeric and monomeric G proteins, also called large and small G proteins, respectively, where the former is composed of three subunits (α, β, and γ) and latter is composed of single subunit (α). Plant growth and development is partially regulated by the combined activity of large and small G proteins. Besides, identifi cation of profuse number of stress-related genes interacting with monomeric and heterotrimeric G-protein complex in both Arabidopsis and rice suggests their involvement in modulation of abiotic stress responses. Further, based on the functional analysis of several G proteins (large and small) regulating cell growth, cell proliferation, seed germination, sugar sensing, and hormonal responses indicate these proteins are equally essential for plant development. Due to increased demand of food crops to sustain growing population in adverse environmental conditions, the role of G proteins and modulation of GTPase signaling has been studied in plants to produce genetically modifi ed crop plants with increased stress tolerance and productivity.

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Section: Rab Gtpasesmentioning

confidence: 99%

Small and Large G Proteins in Biotic and Abiotic Stress Responses in Plants

Pandey

Sharma

Pandey

2015

Elucidation of Abiotic Stress Signaling in Plants

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“…However, in the case of most components of the putative chloroplast vesicle transport system, unequivocal experimental validation of their localization and function is still missing. So far, only two, the small plastid GTPases CPSAR1 and CPRabA5e, have been investigated in molecular detail [47][48][49].…”

Section: Trafficking Of Lipidsmentioning

confidence: 99%

“…The Rab GTPase CPRabA5e is a close homolog of the vesicle transport protein Ypt31/32 from yeast and has recently been shown to complement the ypt31/32 mutant [49]. CPRabA5e localizes to the chloroplast stroma and thylakoids, and an A. thaliana knock-out mutant displayed enhanced chloroplast vesicle formation at low temperatures and under oxidative stress [49].…”

Section: Trafficking Of Lipidsmentioning

confidence: 99%

Biogenesis of thylakoid membranes

Rast

Heinz

Nickelsen

2015

Biochimica et Biophysica Acta (BBA) - Bioenergetics

122

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Thylakoids mediate photosynthetic electron transfer and represent one of the most elaborate energy-transducing membrane systems. Despite our detailed knowledge of its structure and function, much remains to be learned about how the machinery is put together. The concerted synthesis and assembly of lipids, proteins and low-molecular-weight cofactors like pigments and transition metal ions require a high level of spatiotemporal coordination. While increasing numbers of assembly factors are being functionally characterized, the principles that govern how thylakoid membrane maturation is organized in space are just starting to emerge. In both cyanobacteria and chloroplasts, distinct production lines for the fabrication of photosynthetic complexes, in particular photosystem II, have been identified. This article is part of a Special Issue entitled: Chloroplast Biogenesis.

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“…16 Therefore spatial segregation could be an indication of false positive results, or is reflecting the possibility that similar proteins (like the identified ones) are present within the given organelle. Regardless, it is interesting to note that several of the proteins identified, using yeast two-hybrid with CPRabA5e as bait 2 or Interactome 3D, have relations to vesicles, thylakoid formation and/or GTP cycling.…”

Section: Protein Interactionsmentioning

confidence: 99%