A novel colorimetric immunosensor based on platinum colloid nanoparticles immobilized on PowerVision as signal probes and Fe₃O₄@β‐cyclodextrin as capture probes for ractopamine detection in pork

Abstract

Background A novel colorimetric immunosensor was developed for the simple, sensitive and selective detection of ractopamine (RAC) based on using β‐cyclodextrin‐modified Fe3O4 particles (Fe3O4@β‐CD) as capture probes and complex platinum colloid nanoparticles (PtNPs‐PV) composed of platinum colloid nanoparticles (PtNPs) and polymerase chelate PowerVision (PV) as signal probes. Results PtNPs‐PV double catalyzed the chromogenic substrate 3,3′‐diaminobenzidine (DAB), which induced changes in the color of DAB and chromogenic absorbance. Incubation temperature, pH and incubation time were systematically optimized and, under optimum conditions, the measured absorbance values showed a linear relationship with the RAC concentrations in the range 0.03–8.1 ng mL−1. The detection limit was 0.01 ng mL−1. The sensor exhibited high sensitivity and specificity, as demonstrated by testing structurally similar organic compounds such as salbutamol, clenbuterol and dopamine. The practicality of the developed colorimetric immunosensor was supported by the successful detection of RAC in pork samples with recovery ranging from 94.00% to 106.00%. Conclusion We designed a novel sandwich‐type noncompetitive colorimetric immunoassay for the detection of trace levels of RAC in pork. The proposed method can also be used for the detection of toxins in food products via PtNPs‐PV amplification. © 2018 Society of Chemical Industry

Application of Noble Metals in the Advances in Animal Disease Diagnostics

“…β 2 -adrenergic receptor agonists (β2-agonists) is a drug group which is usually used for the treatment of pulmonary diseases in animals [81,82]. However, they can promote animal growth and increase feed efficiency by enhancing protein accretion and reducing fat deposition producing "lean meats" [82][83][84].…”

“…β 2 -adrenergic receptor agonists (β2-agonists) is a drug group which is usually used for the treatment of pulmonary diseases in animals [81,82]. However, they can promote animal growth and increase feed efficiency by enhancing protein accretion and reducing fat deposition producing "lean meats" [82][83][84]. The use of this drug group in veterinary medicine is illegal since prolonged consumption of residues in animal-derived products can cause headache, chest tightness, nausea, and more.…”

“…One of this β2-agonists is ractopamine (RAC), however RAC is a derivative of clenbuterol which causes high blood pressure and heart disease in human. Thus, the use of RAC is illegal in Europe and China but RAC is still used around the world due to its effectiveness and low cost [82]. Thus, detection of RAC and its residue using simple and accurate method is important.…”

“…Thus, detection of RAC and its residue using simple and accurate method is important. Sun et al [82] developed a colorimetric immunosensor based PtNPs immobilized on Power Vision (PV) as signal probes and Fe 3 O 4 @β-cyclodextrin as capture probes for ractopamine detection in pork. PtNPs-PV double catalyzed the chromogenic substrate 3,3′-diaminobenzidine (DAB), which induced changes in DAB's color and chromogenic absorbance.…”

Selective Hydrogenation of the Carbonyls in Furfural and 5-Hydroxymethylfurfural Catalyzed by PtNi Alloy Supported on SBA-15 in Aqueous Solution Under Mild Conditions

“…Furfural and 5-hydroxymethylfurfural are the most important platform chemicals which could be derived from hemicellulose and cellulose via hydrolysis and subsequent dehydration (Mika et al, 2018;Hui et al, 2019;Luo et al, 2019;Chen et al, 2021;Shi et al, 2021). Due to their diverse double bonds, that is, C O and C C, FF and HMF could be further converted into different valuable chemicals (Sharma et al, 2013;Fulajtárova et al, 2015;Sun et al, 2019;Herrera et al, 2020;Zhu et al, 2020). Especially, because of the wide applications of furfuryl alcohol (FFA) and 2,5dihydroxymethylfuran (DHMF) on resins and farm chemicals (Gandini, 1990;Gandini, 2010), the selective conversion of FF/HMF to FFA/DHMF has received much interests.…”