A novel Cry9Aa with increased toxicity for Spodoptera exigua (Hübner)

Naimov, Samir; Nedyalkova, Radostina; Staykov, Nikola; Weemen-Hendriks, Mieke; Minkov, Ivan; Maagd, Ruud A. de

doi:10.1016/j.jip.2013.11.003

Cited by 13 publications

(10 citation statements)

References 12 publications

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“…The susceptibility of S. exigua larvae was estimated for numerous holotype Bt pesticidal proteins of groups Cry1, Cry2 39 and Vip3A 37,40,41 . To date, among all Cry9 proteins, only four have been assessed for their insecticidal activity towards the beet armyworm: Cry9Aa5 and Cry9Ca1 were found to be active and Cry9Aa1 and Cry9Cb showed no activity 11,15,19 . These findings make the Cry9 host specificity issue very puzzling because proteins sharing high sequence similarity gave different results.…”

Section: Discussionmentioning

confidence: 99%

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A novel Bacillus thuringiensisCry9Ea‐like protein with high insecticidal activity towards Cydia pomonella larvae

Baranek

Banaszak

Kaznowski

et al. 2020

Pest Management Science

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BACKGROUND The host specificity of known Bacillus thuringiensis Cry and Vip pesticidal proteins still needs extensive investigation and the proteins currently used in crop protection are not effective against many pest species. Cydia pomonella (L.) is a widespread and economically important pest of apples, very difficult to control, since the larvae spend most of their life inside a fruit. Currently, large amounts of broad‐spectrum, detrimental synthetic agents are used to combat this herbivore and therefore biopesticides with high activity against C. pomonella are very much needed. RESULTS The toxicity of B. thuringiensis Cry9Ea along with five distinct pesticidal proteins (Cry1Aa, Cry1Ca, Cry1Ia, Cry2Ab and Vip3Aa) has been determined towards the first‐instar larvae of C. pomonella. Cry9Ea has much higher activity than the remaining tested proteins (30–1200‐fold lower LC50) and possibly is the most potent B. thuringiensis pesticidal protein bioassayed against C. pomonella so far. In contrast, Cry9Ea is not toxic towards Spodoptera exigua (Hübn.), indicating a potentially narrow spectrum of activity. Both insect species show high variability in susceptibility to the remaining Cry/Vip proteins. CONCLUSIONS The obtained results extend the existing knowledge regarding B. thuringiensis pesticidal protein host range and indicate Cry9Ea as a promising candidate for successful biological control of C. pomonella. © 2020 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

“…Only a few Cry9 proteins have been tested for their activity and the assessments were done against a very limited number of pest species. [11][12][13][14][15][16][17][18][19] More research is needed to broaden knowledge of the Cry9 pesticidal proteins host range and comprehensively estimate their possible use in pest control.…”

Section: Introductionmentioning

confidence: 99%

A novel Bacillus thuringiensisCry9Ea‐like protein with high insecticidal activity towards Cydia pomonella larvae

Baranek

Banaszak

Kaznowski

et al. 2020

Pest Management Science

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“…However, the results also indicated that the homozygous line was susceptible to M. brassicae and S. exigua . It was reported that the cry1C gene from Bt could provide effective protection against the Spodoptera species and the Mamestra species in transgenic plants . Therefore, A. tumefaciens ‐mediated transformation of cabbage inbred line A21‐3 with a binary vector carrying the synthetic cry1C gene is being carried out in our laboratory.…”

Section: Discussionmentioning

confidence: 99%

High resistance of transgenic cabbage plants with a synthetic cry1Ia8 gene from Bacillus thuringiensis against two lepidopteran species under field conditions

Yang

Tang

et al. 2015

Pest. Manag. Sci.

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“…Vectors pPB08 (cry1Aa), pB03 (cry1Ac), pBD150 (cry1Ca), pMH15 (cry1Da), pMH21 (cry1Fa), pBD172 (cry1Ia), pCIz1 (cry9Aa5) and pSN19 (hybrid protein SN19 with domain composition 1Ba/1Ia/1Ba) were used in this study. All vectors are thoroughly described elsewhere (Bosch et al, 1994;de Maagd et al, 1999;de Maagd et al, 1996;de Maagd et al, 2000;Naimov et al, 2014;Naimov et al, 2001). Protein was partially purified from inclusion bodies according to (Herrero et al, 2004).…”

Section: Preparation Of Bt Cry Protein Variantsmentioning

confidence: 99%

Toxicity of Bacillus thuringiensis (L.) Cry proteins against summer fruit tortrix (Adoxophyes orana - Fischer von Rösslerstamm)

Radosavljević

Naimov

2016

Journal of Invertebrate Pathology

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The activity of seven Cry1, one Cry9 and one hybrid Cry1 protoxins against neonate larvae of summer fruit tortrix (Adoxophyes orana - Fischer von Rösslerstamm) has been investigated. Cry1Ia is identified as the most toxic protein, followed by Cry1Aa and Cry1Ac. Cry1Ca, Cry1Cb, Cry1Da and Cry1Fa were less active, while SN19 (Cry1 hybrid protein with domain composition 1Ba/1Ia/1Ba) and Cry9Aa exhibited negligible toxicity against A. orana. In vitro trypsin-activated Cry1Ac is still less active than Cry1Ia protoxin, suggesting that toxicity of Cry1Ia is most probably due to more complex differences in further downstream processing, toxin-receptor interactions and pore formation in A. orana's midgut epithelium.

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A novel Cry9Aa with increased toxicity for Spodoptera exigua (Hübner)

Cited by 13 publications

References 12 publications

A novel Bacillus thuringiensisCry9Ea‐like protein with high insecticidal activity towards Cydia pomonella larvae

A novel Bacillus thuringiensisCry9Ea‐like protein with high insecticidal activity towards Cydia pomonella larvae

High resistance of transgenic cabbage plants with a synthetic cry1Ia8 gene from Bacillus thuringiensis against two lepidopteran species under field conditions

Toxicity of Bacillus thuringiensis (L.) Cry proteins against summer fruit tortrix (Adoxophyes orana - Fischer von Rösslerstamm)

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