A novel DAD type and folic acid conjugated fluorescent monomer as a targeting probe for imaging of folate receptor overexpressed cells

Abstract

We describe a modification and post-functionalization technique for a donor-acceptor-donor type monomer; 6-(4,7-bis(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-2H-benzo[d][1,2, 3]triazol-2-yl)hexan-1-amine. Folic acid was attached to the fluorescent structure. The conjugation was confirmed via NMR and Fourier transform infrared analyses. Cytotoxicity was investigated and the comparison of association of targeted monomeric structures in tumor cells was monitored via fluorescence microscopy.

Recent advances in small-molecule fluorescent probes with the function of targeting cancer receptors

“…In 2014, Kanik et al reported a fluorescent probe 38 targeting folic acid receptors. 112 The dye used by the probe forms a donor–acceptor–donor structure to produce fluorescence. After coupling the monomer with folic acid, the probe has good targeting ability for cancer cells (Fig.…”