A Novel Delivery System for the Controlled Release~of Antimicrobial Peptides: Citropin 1.1 and Temporin A

Piotrowska, Urszula; Olędzka, Ewa; Zgadzaj, Anna; Bauer, Marta; Sobczak, Marcin

doi:10.3390/polym10050489

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…Moreover, the qRT-PCR result explained that GERM CLEAN impaired virulence of S. mutans through downregulating expression of EPS-and acid-production related genes. e antibacterial activity of GERM CLEAN was preliminarily verified through the antibacterial ring test, but the diameter of inhibition zones formed by GERM CLEAN was not very stable, which varied from 8.4 mm to 12 mm according to our repeated tests, and we suspected that it might be related to the variety of the peptide stability [64][65][66][67][68][69], and this hypothesis needs further confirmation.…”

Section: Discussionmentioning

confidence: 97%

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

Xiong

Chen

et al. 2020

BioMed Research International

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Objective. To investigate the antibacterial effect of a novel antimicrobial peptide containing oral spray GERM CLEAN on Streptococcus mutans (S. mutans) in vitro and further explore the related mechanisms at phenotypic and transcriptional levels. Methods. The disk diffusion method was used to preliminarily appraise the antimicrobial effect of GERM CLEAN. The minimal inhibitory concentration (MIC) of GREM CLEAN towards S. mutans was determined by the broth dilution method. S. mutans virulence-related phenotypic assays including initial adhesive assay, pH drop, exopolysaccharides (EPS), and biofilm formation measurements and quantitative real-time PCR (qRT-PCR) were further applied to detect the inhibitory mechanisms of GREM CLEAN at 1/2MIC. Results. The diameter (10.18 ± 1.744 mm) of inhibition zones formed by GERM CLEAN preliminarily indicated its inhibitory effect on the major cariogenic bacteria S. mutans. The minimal inhibitory concentration of GERM CLEAN on S. mutans was 100% mass fraction (the stock solution). The study of the antibacterial mechanism showed that GERM CLEAN had a certain inhibitory effect on the initial adhesion, acid production, extracellular polysaccharides (EPS) production, and biofilm formation of S. mutans. GERM CLEAN disturbed S. mutans biofilm physiology mainly through destruction of biofilm architecture and suppression of bacterial growth. The results of qRT-PCR further confirmed that the expression levels of EPS and lactic acid generation genes including gtfB, gtfC, gtfD, and ldh were significantly repressed by treating with GERM CLEAN, and this was consistent with our phenotypic results. Conclusion. The novel antimicrobial peptide containing oral spray GERM CLEAN has an anti-Streptococcus mutans effect and the inhibitory property may be due to suppression of the virulence factors of S. mutans including adhesive, acidogenicity, EPS, and biofilm formation.

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Section: Discussionmentioning

confidence: 97%

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

Xiong

Chen

et al. 2020

BioMed Research International

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“…To improve AMPs administration, new polymeric carriers can be used as highly controlled release devices. Piotrowska et al [81] chose the peptides citropin 1.1 (CIT) and TA for their experiments. They found that the release rate of the active pharmaceutic ingredients (APIs) was strongly dependent on the API characteristics and the matrix microstructure.…”

Section: Temporin Amentioning

confidence: 99%

Temporins: An Approach of Potential Pharmaceutic Candidates

Romero

Cardillo

Ceron

et al. 2020

Surgical Infections

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Antimicrobial peptides (AMPs), also known as host defense peptides, are small and mostly polycationic molecules that form part of the innate immune response. There are currently more than 3000 experimentally reported AMPs. Particularly in frogs, the temporin family has been discovered as potential AMPs. The aim of this work is to review the latest publications about this class of peptides, discuss their properties, and present an update of the last studies and new discoveries in the field. More than 130 temporins have been identified in this family. The most studied temporins are temporin A (TA), temporin B (TB), and temporin L (TL). These peptides showed antimicrobial activity against gram-negative, gram-positive bacteria and fungi. Since the discovery of temporins in 1996, several groups of researchers isolated different peptides from various species of frogs that were included as members of this family. Although antimicrobial activity of many temporins has not been analyzed yet, most of them showed antimicrobial and antifungal activities. A combination of nanotechnology and AMPs for temporins in different antimicrobial treatments could be a promising alternative for resistant pathogens. These studies demonstrate that, even with the advancement in scientific research on the composition and antimicrobial activity of temporins, further studies are necessary to wholly understand their components and mechanisms of action.

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“…PCL-1 with X c of 50.5% that have a high content of non-charged macrocycles in their structures (ca. 35% [ 9 ]), thus the EE in this case was lower. In contrast, we noted higher EE values for MP-PCL-2 ( X c = 56.3%) with a lower content of macrocyclic chains (19% [ 9 ]).…”

Section: Resultsmentioning

confidence: 95%

“…This study is a continuation of our previous research [ 9 ] on the synthesis and investigation of the structural, physicochemical and biological properties of the PCLs matrices for prolonged peptide release. In this paper, medium-term delivery systems of citropin 1.1 (CIT, amino acids sequence: GLFDVIKKVASVIGGL) were prepared from poly(ε-caprolactone) (PCL) matrices using the solvent evaporation method.…”

Section: Introductionmentioning

confidence: 79%

“…PCL-1 ( M n = 6100 g·mol −1 ; Đ = 1.8; X c = 50.5%) and PCL-2 ( M n = 5700 g·mol −1 , Đ = 1.5, X c = 56.3%) matrices were obtained by the enzymatic ring-opening polymerization (eROP) of ε-caprolactone (CL) in the presence of Candida antarctica lipase B (CALB) as catalyst in two ionic liquids (ILs): [bmim][NTf 2 ] and [bmim][PF 6 ] according to our previously described method [ 9 ]. CIT (≥ 95%) was purchased from Lipopharm.pl (Gdansk, Poland) [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

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The Effect of Polymer Microstructure on Encapsulation Efficiency and Release Kinetics of Citropin 1.1 from the Poly(ε-caprolactone) Microparticles

et al. 2018

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Cationic antimicrobial peptides represent a promising therapeutic option against multidrug-resistant bacteria for the treatment of local infections. However, due to their low stability and potential toxicity, there are limited possibilities for their application in clinical practice. In this study, different poly(ε-caprolactone) (PCL) microparticles (MPs) loaded with citropin 1.1 (CIT) were investigated in order to demonstrate the effect of the polymer microstructure on the encapsulation efficiency (EE) and kinetics of the peptide release from the newly developed devices. The characteristics of the new systems in terms of surface morphology, particle size, EE and zeta potential analysis, as well as the haemolytic activities of the peptide were investigated. The in vitro release kinetics of CIT from the MPs was also investigated. CIT loading was favoured by a high content of negative charged linear polymer chains in the PCL structure. The presence of non-charged, amorphous macrocycle domains results in faster degradation of the PCL matrix. Depending on the crystallinity of the PCL, the peptide release exhibited a near-zero-order or near-first-order profile with no “burst release”. The results indicated that CIT-loaded PCL MPs could potentially be a promising drug delivery system (DDS) for the treatment of local infections.

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A Novel Delivery System for the Controlled Release~of Antimicrobial Peptides: Citropin 1.1 and Temporin A

Cited by 11 publications

References 32 publications

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

Temporins: An Approach of Potential Pharmaceutic Candidates

The Effect of Polymer Microstructure on Encapsulation Efficiency and Release Kinetics of Citropin 1.1 from the Poly(ε-caprolactone) Microparticles

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