A novel device to measure static hindlimb weight-bearing forces in pronograde rodents

Williams, Morika D.; Sommer, Samantha; Meyers, Rachel C.; Valdivia, J. A.; Nolan, Michael W.; Lascelles, B. Duncan X.

doi:10.1016/j.jneumeth.2019.108405

Cited by 7 publications

(10 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, in the fields of nerve tissue regeneration and orthopedic research, evaluating the restoration of the leg function is performed by using the weight-bearing assessment. [64,65] This study histologically evaluated the muscle regeneration through Gel NF in vivo. In addition, it is worthwhile to evaluate the recovery of the leg function along with muscle regeneration by evaluating the weight-bearing during the recovery process.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Stretchable Nanofibrous Sheets with Sacrificial Coaxial Electrospinning for Treatment of Traumatic Muscle Injury

Pham-Nguyen

Son

Kwon

et al. 2021

Adv Healthcare Materials

View full text Add to dashboard Cite

Traumatic muscle injury with massive loss of muscle volume requires intramuscular implantation of proper scaffolds for fast and successful recovery. Although many artificial scaffolds effectively accelerate formation and maturation of myotubes, limited studies are showing the therapeutic effect of artificial scaffolds in animal models with massive muscle injury. In this study, improved myotube differentiation is approved on stepwise stretched gelatin nanofibers and applied to damaged muscle recovery in an animal model. The gelatin nanofibers are fabricated by a two-step process composed of co-axial electrospinning of poly(ɛ-caprolactone) and gelatin and subsequent removal of the outer shells. When stepwise stretching is applied to the myoblasts on gelatin nanofibers for five days, enhanced myotube formation and polarized elongation are observed. Animal models with volumetric loss at quadriceps femoris muscles (>50%) are transplanted with the myotubes cultivated on thin and flexible gelatin nanofiber. Treated animals more efficiently recover exercising functions of the leg when myotubes and the gelatin nanofiber are co-implanted at the injury sites. This result suggests that mechanically stimulated myotubes on gelatin nanofiber is therapeutically feasible for the robust recovery of volumetric muscle loss. IntroductionVolumetric muscle loss (VML) is a form of severe skeletal muscle damage that can occur if there is more than 20% volume loss that

show abstract

Section: Resultsmentioning

confidence: 99%

Preparation of Stretchable Nanofibrous Sheets with Sacrificial Coaxial Electrospinning for Treatment of Traumatic Muscle Injury

Pham-Nguyen

Son

Kwon

et al. 2021

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…A separate cohort of mice received an intra-articular injection of either MIA (1 mg/10 μL) or saline (10 μL). A static horizontal incapacitance meter (SHIM) (15) was used to assess the percent of hindlimb supported body weight placed on each hindlimb. Mice placed significantly less weight on the injected limb compared to the contralateral limb starting at Day 2 and continuing to Day 28 (Figure 2a).…”

Section: Resultsmentioning

confidence: 99%

“…Static weight bearing was measured using a SHIM incapacitance meter (IC Meter) connected to a system 8000 micro-measurements tool (15). Data were collected from mice as described previously (15).…”

Section: Methodsmentioning

confidence: 99%

“…SHIM incapacitance meter to measure static limb use: Static weight bearing was measured using a SHIM incapacitance meter (IC Meter) connected to a system 8000 micro-measurements tool (15). Data were collected from mice as described previously (15). Data were retained if the animal stood still in relaxed position, without noticeably shifting weight, lifting or offloading a limb, or turning the head.…”

Section: Behaviormentioning

confidence: 99%

See 1 more Smart Citation

Artemin and its cognate receptor, GFRα3, play a function role in osteoarthritis pain

Minnema

Mishra

Lascelles

2020

Preprint

View full text Add to dashboard Cite

Osteoarthritis associated pain (OA-pain) is a significant global problem. OA-pain limits limb use and mobility, and is associated with widespread sensitivity. Therapeutic options are limited, and the ones that are available are often associated with side or adverse effects. The lack of therapeutic options is partly due to a lack of understanding of clinically relevant underlying neural mechanisms of OA-pain. In previous work in naturally occurring OA-pain in dogs, we identified potential signaling molecules (artemin/GFRα3) that were upregulated. Here, we use multiple approaches including knockout mice, immunological suppression in a mouse model of OA, and clinically relevant measures of sensitivity and limb use to explore the functional role of artemin/GFRα3 signaling in OA-pain. We found the monoiodoacetate (MIA)-induced OA model in mice is associated with decreased limb use and hypersensitivity. GFRα3 expression is increased in sensory neurons. Exogenous artemin induces heat, cold and mechanical hypersensitivity, and anti-artemin monoclonal antibody administration reverses this hypersensitivity and restores limb use in mice with MIA-induced OA pain. Our results provide a molecular basis of arthritis pain linked with artemin/GFRα3 signaling and indicate that further work is warranted to investigate the neuronal plasticity and the pathways that drive pain in OA.

show abstract

“…Static weight-bearing was measured using a Static Hind limb Incapacitance Meter (SHIM) connected to a system 8000 MicroMeassurements tool (Williams et al, 2019), and also measured using the IITC Incapacitance Meter (IC Meter) (IITC, Life Science, Woodland Hills, CA, United States). For the SHIM, data were collected from mice as described previously (Williams et al, 2019). Data were retained if the animal stood still in a relaxed position, without noticeably shifting weight, lifting, offloading a limb, or turning the head.…”

Section: Incapacitance Meter To Measure Static Limb Usementioning

confidence: 99%

Investigating the Role of Artemin and Its Cognate Receptor, GFRα3, in Osteoarthritis Pain

et al. 2022

Self Cite

View full text Add to dashboard Cite

Osteoarthritis (OA) associated pain (OA-pain) is a significant global problem. OA-pain limits limb use and mobility and is associated with widespread sensitivity. Therapeutic options are limited, and the available options are often associated with adverse effects. The lack of therapeutic options is partly due to a lack of understanding of clinically relevant underlying neural mechanisms of OA-pain. In previous work in naturally occurring OA-pain in dogs, we identified potential signaling molecules (artemin/GFRα3) that were upregulated. Here, we use multiple approaches, including cellular, mouse genetic, immunological suppression in a mouse model of OA, and clinically relevant measures of sensitivity and limb use to explore the functional role of artemin/GFRα3 signaling in OA-pain. We found the monoiodoacetate (MIA)-induced OA-pain in mice is associated with decreased limb use and hypersensitivity. Exogenous artemin induces mechanical, heat, and cold hypersensitivity, and systemic intraperitoneal anti-artemin monoclonal antibody administration reverses this hypersensitivity and restores limb use in mice with MIA-induced OA-pain. An artemin receptor GFRα3 expression is increased in sensory neurons in the MIA model. Our results provide a molecular basis of arthritis pain linked with artemin/GFRα3 signaling and indicate that further work is warranted to investigate the neuronal plasticity and the pathways that drive pain in OA.

show abstract

A novel device to measure static hindlimb weight-bearing forces in pronograde rodents

Cited by 7 publications

References 56 publications

Preparation of Stretchable Nanofibrous Sheets with Sacrificial Coaxial Electrospinning for Treatment of Traumatic Muscle Injury

Preparation of Stretchable Nanofibrous Sheets with Sacrificial Coaxial Electrospinning for Treatment of Traumatic Muscle Injury

Artemin and its cognate receptor, GFRα3, play a function role in osteoarthritis pain

Investigating the Role of Artemin and Its Cognate Receptor, GFRα3, in Osteoarthritis Pain

Contact Info

Product

Resources

About