A Novel Disease (Water Bubble Disease) of the Giant Freshwater Prawn Macrobrachium rosenbergii Caused by Citrobacter freundii: Antibiotic Treatment and Effects on the Antioxidant Enzyme Activity and Immune Responses

Zhao, Caiyuan; Wen, Huagen; Huang, Shengsheng; Weng, Shaoping; He, Jianguo

doi:10.3390/antiox11081491

Cited by 13 publications

(11 citation statements)

References 112 publications

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“…freundii is a common aquaculture pathogen that is able to infect aquatic animals and lead to high mortality. , For example, C. freundii could cause water bubble disease in Macrobrachium rosenbergii and bring some infectious diseases in Acipenser sinensis and carp fish. , Besides, the transfer assay of pWWTP-01 was also performed under surface water conditions (Text S1). Although the transfer frequency was lower than the laboratory condition after 16 h, the frequency could still reach 10 –6 after 48 h, which suggested that plasmid pWWTP-01 carried by DRB could also transfer in the surface water condition (Figure S5).…”

Section: Resultsmentioning

confidence: 99%

Deciphering Multidrug-Resistant Plasmids in Disinfection Residual Bacteria from a Wastewater Treatment Plant

Lin,

Zhou,

Shuai

et al. 2024

Environ. Sci. Technol.

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Current disinfection processes pose an emerging environmental risk due to the ineffective removal of antibioticresistant bacteria, especially disinfection residual bacteria (DRB) carrying multidrug-resistant plasmids (MRPs). However, the characteristics of DRB-carried MRPs are poorly understood. In this study, qPCR analysis reveals that the total absolute abundance of four plasmids in postdisinfection effluent decreases by 1.15 log units, while their relative abundance increases by 0.11 copies/cell compared to investigated wastewater treatment plant (WWTP) influent. We obtain three distinctive DRB-carried MRPs (pWWTP-01−03) from postdisinfection effluent, each carrying 9−11 antibiotic-resistant genes (ARGs). pWWTP-01 contains all 11 ARGs within an ∼25 Kbp chimeric genomic island showing strong patterns of recombination with MRPs from foodborne outbreaks and hospitals. Antibiotic-, disinfectant-, and heavy-metal-resistant genes on the same plasmid underscore the potential roles of disinfectants and heavy metals in the coselection of ARGs. Additionally, pWWTP-02 harbors an adhesin-type virulence operon, implying risks of both antibiotic resistance and pathogenicity upon entering environments. Furthermore, some MRPs from DRB are capable of transferring and could confer selective advantages to recipients under environmentally relevant antibiotic pressure. Overall, this study advances our understanding of DRB-carried MRPs and highlights the imminent need to monitor and control wastewater MRPs for environmental security.

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Section: Resultsmentioning

confidence: 99%

Deciphering Multidrug-Resistant Plasmids in Disinfection Residual Bacteria from a Wastewater Treatment Plant

Lin,

Zhou,

Shuai

et al. 2024

Environ. Sci. Technol.

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“…protein, which destroys and eliminates foreign bodies invading the body by hydrolyzing bacterial peptidoglycan, and takes the defense function of the body (Zhao et al, 2022). SOD is a crucial component of antioxidant enzymes, as an active oxygen scavenger, which can scavenge free radicals in the body and enhance the phagocytosis of macrophages.…”

Section: Discussionmentioning

confidence: 99%

Study on immunogenicity of Lrp subunit vaccine against Vibrio alginolyticus in pearl gentian grouper(♀Epinephelus fuscoguttatus ×♂Epinephelus lanceolatus)

Wan

Ding

2023

Front. Mar. Sci.

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Vibrio alginolyticus is the main pathogen causing vibriosis in pearl gentian grouper, which has caused significant financial losses to farmers. To develop a sustainable and effective subunit vaccine for the prevention and control of vibriosis, Lrp recombinant protein from V. alginolyticus was expressed and purified in this study. Western Blotting and ELISA demonstrated that Lrp recombinant protein with relatively higher antigenicity in V. alginolyticus can be used as an antigen for the subunit vaccine. Chitosan oligosaccharide (COS) is a very potential aquatic vaccine adjuvant to boost the immunological protection of the vaccine. Therefore, to evaluate the immune response and protection of the subunit vaccine against V. alginolyticus in pearl gentian grouper, we designed the Lrp group and Lrp+COS group as experimental groups with PBS as the control group. Immunological testing revealed that grouper serum from the experimental group had significantly higher levels of the particular antibody IgM, lysozyme (LZM), catalase (CAT), and superoxide dismutase (SOD) than serum from the control group. Additionally, groupers from the experimental group showed higher immune gene expression levels, namely IgM, CD8α, MHC-Iα, IL-1β, IL-16, and TNF-α. After the challenge experiment, the immune protection rates of the Lrp group and Lrp+COS group were respectively enhanced to 60% and 72%. The aforementioned findings demonstrated that the Lrp+COS group’s immunological impact was superior to that of the Lrp group. Therefore, the Lrp+COS subunit vaccine is a promising candidate for the prevention and management of vibrio infection in pearl grouper.

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“…91 Despite this, a 2022 study found C. freundii to be associated with juvenile mortalities of up to 30% in grow-out ponds in China, with laboratory challenges with C. freundii replicating clinical signs of disease seen in culture settings. 108 Typically, prawns infected with C. freundii develop a 7mm diameter bubble under the carapace, show loss of appetite, weight loss and inactivity. Clinical signs of disease are quickly followed by mortality and spread of the disease to other prawns.…”

Section: Notementioning

confidence: 99%

“…115 Citrobacter C. freundii 108 Juveniles (GO, L) "Water bubble disease (WDB)-Formation of a "water bubble" with a diameter of c. 7 mm under the carapace, loss of appetite, inactivity, weight loss. 108 Not described.…”

Section: Histopathological Signs Of Infectionmentioning

confidence: 99%

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Diseases of the giant river prawnMacrobrachium rosenbergii: A review for a growing industry

Hooper

Debnath

Stentiford

et al. 2022

Reviews in Aquaculture

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The giant river prawn, Macrobrachium rosenbergii, is a major focus of aquaculture in tropical and sub-tropical regions around the globe. Over the last 30 years, culture of M. rosenbergii has increased exponentially as demand has risen both for domestic consumption and for international export trade. As with many aquaculture species increases in production have been accompanied by the emergence of diseases affecting yield, profit and trading potential. Disease-causing agents include pathogens infecting other crustaceans, such as Decapod Iridescent Virus (DIV1), as well as pathogens only known from M. rosenbergii such as White Tail Disease caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV). Here, we review the pathogenic agents associated with the culture of M. rosenbergii since commercial culture began in earnest during the 1970s. Particular emphasis is given to pathogens first identified in other aquaculture host species, but which have subsequently been shown to infect and cause disease in M. rosenbergii. As polyculture of M. rosenbergii with other aquaculture species is common practice, including culture with other decapods, crabs and fish, increased pathogen transfer among these farmed species may occur as M. rosenbergii aquaculture increases in the future.aquaculture, emerging disease, giant river prawn, polyculture | INTRODUCTIONAquaculture is the fastest-growing farmed food sector, with the proportion of cultured to caught seafood increasing year-on-year. 1,2 Global aquaculture production for fish, crustaceans and molluscs surpassed 87 million tonnes in 2020, just under half of all world production. 2 Crustacean production by aquaculture far surpasses that by capture, with more than 11,000,000 tonnes in 2020 from aquaculture compared to 6,000,000 tonnes by capture. 2 Despite these considerable production figures, it is estimated that up to 40% of tropical shrimp aquaculture production is lost annually, 3 equating to losses of over 3 billion USD, and this is primarily due to infection with viral agents. 4 Losses on this scale threaten global food security, with most of the consumption of cultured crustaceans being outside of producing countries, making management and mitigation of disease of key importance to the future success of crustacean aquaculture. 4 Stentiford et al. (2012) outlines a holistic strategy involving both producer and consumer nations to improve husbandry and farm management, better understand pathogens and their spread (either by movement of animals or alternative hosts) and learn lessons from previous disease outbreaks. This review focuses on these points to outline the pathogenic agents in Macrobrachium rosenbergii culture, identify other hosts

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A Novel Disease (Water Bubble Disease) of the Giant Freshwater Prawn Macrobrachium rosenbergii Caused by Citrobacter freundii: Antibiotic Treatment and Effects on the Antioxidant Enzyme Activity and Immune Responses

Cited by 13 publications

References 112 publications

Deciphering Multidrug-Resistant Plasmids in Disinfection Residual Bacteria from a Wastewater Treatment Plant

Deciphering Multidrug-Resistant Plasmids in Disinfection Residual Bacteria from a Wastewater Treatment Plant

Study on immunogenicity of Lrp subunit vaccine against Vibrio alginolyticus in pearl gentian grouper(♀Epinephelus fuscoguttatus ×♂Epinephelus lanceolatus)

Diseases of the giant river prawnMacrobrachium rosenbergii: A review for a growing industry

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