A novel dual-frequency loading system for studying mechanobiology of load-bearing tissue

Zhang, Chunqiu; Qiu, Lulu; Gao, Lilan; Guan, Yingjie; Xu, Qiang; Zhang, Xizheng; Chen, Qian

doi:10.1016/j.msec.2016.06.080

Cited by 5 publications

(2 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are the two main forms of relative motions between the femoral condyle and the underlying tibia during joint movement, rolling and sliding, exerting compression and shear stress separately on the contacting cartilage. Compression transmitted by solid medium is the most widely studied mechanical stimulus for reconstructing the articular cartilage, especially the cartilage at knee joint, the largest load-bearing joint in human body [ [389] , [390] , [391] , [392] , [393] , [394] ]. Wang et al cultured the multilayer composite scaffold loaded with BMSCs in a bioreactor under a dynamic compression.…”

Section: Other Key Elements In Cartilage and Osteochondral Tissue Engineeringmentioning

confidence: 99%

Articular cartilage and osteochondral tissue engineering techniques: Recent advances and challenges

Wei

Dai

2021

Bioactive Materials

194

161

View full text Add to dashboard Cite

In spite of the considerable achievements in the field of regenerative medicine in the past several decades, osteochondral defect regeneration remains a challenging issue among diseases in the musculoskeletal system because of the spatial complexity of osteochondral units in composition, structure and functions. In order to repair the hierarchical tissue involving different layers of articular cartilage, cartilage-bone interface and subchondral bone, traditional clinical treatments including palliative and reparative methods have showed certain improvement in pain relief and defect filling. It is the development of tissue engineering that has provided more promising results in regenerating neo-tissues with comparable compositional, structural and functional characteristics to the native osteochondral tissues. Here in this review, some basic knowledge of the osteochondral units including the anatomical structure and composition, the defect classification and clinical treatments will be first introduced. Then we will highlight the recent progress in osteochondral tissue engineering from perspectives of scaffold design, cell encapsulation and signaling factor incorporation including bioreactor application. Clinical products for osteochondral defect repair will be analyzed and summarized later. Moreover, we will discuss the current obstacles and future directions to regenerate the damaged osteochondral tissues.

show abstract

Section: Other Key Elements In Cartilage and Osteochondral Tissue Engineeringmentioning

confidence: 99%

Articular cartilage and osteochondral tissue engineering techniques: Recent advances and challenges

Wei

Dai

2021

Bioactive Materials

194

161

View full text Add to dashboard Cite

show abstract

“…[11][12][13] In mechanobiological studies, an appropriate loading system is crucial for simulating mechanical signals in the natural environment. 14 In vitro mechanical loading devices have been developed to study cell survival, differentiation, and proliferation. [15][16][17] These devices typically comprise driving, transmission, and culturing chamber modules.…”

Section: Introductionmentioning

confidence: 99%

Parametric optimization of culture chamber for cell mechanobiology research

Guo,

Wang,

Gao

et al. 2023

Exp Biol Med (Maywood)

Self Cite

View full text Add to dashboard Cite

Mechanical signals influence the morphology, function, differentiation, proliferation, and growth of cells. Due to the small size of cells, it is essential to analyze their mechanobiological responses with an in vitro mechanical loading device. Cells are cultured on an elastic silicone membrane substrate, and mechanical signals are transmitted to the cells by the substrate applying mechanical loads. However, large areas of non-uniform strain fields are generated on the elastic membrane, affecting the experiment’s accuracy. In the study, finite-element analysis served as the basis of optimization, with uniform strain as the objective. The thickness of the basement membrane and loading constraints were parametrically adjusted. Through finite-element cycle iteration, the “M” profile basement membrane structure of the culture chamber was obtained to enhance the uniform strain field of the membrane. The optimized strain field of culture chamber was confirmed by three-dimensional digital image correlation (3D-DIC) technology. The results showed that the optimized chamber improved the strain uniformity factor. The uniform strain area proportion of the new chamber reached 90%, compared to approximately 70% of the current chambers. The new chamber further improved the uniformity and accuracy of the strain, demonstrating promising application prospects.

show abstract