A novel electrochemical biosensor with molecularly imprinted polymers and aptamer-based sandwich assay for determining amyloid-β oligomer

You, Min; Yang, Shuai; An, Yu; Zhang, Fan; He, Pingang

doi:10.1016/j.jelechem.2020.114017

Cited by 64 publications

(29 citation statements)

References 48 publications

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“…oligomer showed strong linear correlation ranging between 5 pg/mL-10 ng/mL (R 2 =0.997), where the LoD was calculated at 1.22 pg/mL. 161,166 Thus, the response of the biosensor correlates to an amyloidoligomer concentration range within the detection range found in physiological serum of Alzheimer's 𝛽 disease patients (1.35-12.5 ng/mL). 167 The preparation of 12 amyloid-1-42 MIP biosensors under identical conditions demonstrated a relative 𝛽 standard deviation (RSD) value of 7.7% for the detection of 1 ng/mL of amyloid-1-42 oligomer, 𝛽 indicating that the MIP could be reproduced satisfactorily.…”

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confidence: 81%

Molecularly imprinted polymers in diagnostics: accessing analytes in biofluids

2022

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confidence: 81%

Molecularly imprinted polymers in diagnostics: accessing analytes in biofluids

2022

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“…A new electrochemical biosensor was constructed for the quantification of Aβ 42 oligomers based on the sandwich method of molecularly imprinted polymers and DNA aptamers. The biosensor obtained the improved detection sensitivity (LOD: ~0.27 pM) 99 . In present, Liao et al developed a novel dual-amplification strategy based on exonuclease III-assisted DNA recycling and rolling circle amplification (RCA) for more sensitive detection of Aβ oligomer 100 .…”

Section: The Applications Of Aptamer In Aβ Detectionmentioning

confidence: 97%

Advances in aptamers against Aβ and applications in Aβ detection and regulation for Alzheimer's disease

Zheng¹,

Zhang²,

Zhao³

et al. 2022

Theranostics

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Alzheimer's disease (AD) is an irreversible neurodegenerative disease, causing profound social and economic implications. Early diagnosis and treatment of AD have faced great challenges due to the slow and hidden onset. β-amyloid (Aβ) protein has been considered an important biomarker and therapeutic target for AD. Therefore, non-invasive, simple, rapid and real-time detection methods for AD biomarkers are particularly favored. With the development of Aβ aptamers, the specific recognition between aptamers and Aβ plays a significant role in AD theranostics. On the one hand, aptamers are applied to construct biosensors for Aβ detection, which provides possibilities for early diagnosis of AD. On the other hand, aptamers are used for regulating Aβ aggregation process, which provides potential strategies for AD treatment. Many excellent reviews have summarized aptamers for neurodegenerative diseases or biosensors using specific recognition probes for Aβ detection applications in AD. In this review, we highlight the crucial role of the design, classification and applications of aptamers on Aβ detection as well as inhibition of Aβ aggregation for AD.

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“…Moreover, a sandwich assay electrochemical biosensor was developed by You et al [ 156 ] for the detection of amyloid-β oligomer (AßO), known as the biomarker of Alzheimer’s disease, through the formation of a sandwich composed of an aptamer and a MIP. In detail, core-shell nanoparticles, made up of silver and silica, presented the aptamer on their own surface.…”

Section: Molecularly Imprinted Polymers (Mips)mentioning

confidence: 99%

The Evolution of Molecular Recognition: From Antibodies to Molecularly Imprinted Polymers (MIPs) as Artificial Counterpart

Parisi

Francomano

Dattilo

et al. 2022

JFB

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Molecular recognition is a useful property shared by various molecules, such as antibodies, aptamers and molecularly imprinted polymers (MIPs). It allows these molecules to be potentially involved in many applications including biological and pharmaceutical research, diagnostics, theranostics, therapy and drug delivery. Antibodies, naturally produced by plasma cells, have been exploited for this purpose, but they present noticeable drawbacks, above all production cost and time. Therefore, several research studies for similar applications have been carried out about MIPs and the main studies are reported in this review. MIPs, indeed, are more versatile and cost-effective than conventional antibodies, but the lack of toxicity studies and their scarce use for practical applications, make it that further investigations on this kind of molecules need to be conducted.

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A novel electrochemical biosensor with molecularly imprinted polymers and aptamer-based sandwich assay for determining amyloid-β oligomer

Cited by 64 publications

References 48 publications

Molecularly imprinted polymers in diagnostics: accessing analytes in biofluids

Molecularly imprinted polymers in diagnostics: accessing analytes in biofluids

Advances in aptamers against Aβ and applications in Aβ detection and regulation for Alzheimer's disease

The Evolution of Molecular Recognition: From Antibodies to Molecularly Imprinted Polymers (MIPs) as Artificial Counterpart

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