A novel electrochemical conducting polymer sensor for the rapid, selective and sensitive detection of biothiols

Abstract

Biological thiols (biothiols) in cells and bodily fluids are intrinsically linked to the functioning of important enzymes, deficiency in which can lead to a wide range of physiological and pathological...

Conducting Polymer-Coated Carbon Cloth Captures and Releases Extracellular Vesicles by a Rapid and Controlled Redox Process

“…The acetylthiomethyl groups on EDOTSAc mers were reductively converted into EDOTSH (Figure S3B), affording thiol (−SH) functionalities on the terpolymer-coated carbon cloth [P­(EDOT -co- EDOTSH -co- EDOTEG)], Figure . The evidence of successful activation of [P­(EDOT -co- EDOT-SH -co- EDOTEG) terpolymer], albeit of different compositions and coated on a different substrate was recently reported from our group . The activated surface was then exposed to an oxidation potential of +1.0 V for 60 s (Figure S3C), in a 5 mM solution of MHA and 0.1 M LiClO 4 in ethanol to form disulfide linkages between MHAs and the thiolated moieties (Figure ).…”

“…A fluorescent Alexa Fluor 488-antibody was then conjugated to the surface through the activated carboxylic acid groups of MHAs by EDC-NHS. The release of the fluorescent antibody was then performed by reductive cleavage of the disulfide bonds by applying a potential of −0.8 V for 2 min (Figure S3D) in PBS solution Figure A shows the fluorescence spectra of Alexa Fluor 488 in the release solution, with and without (as a control) EDC-NHS activation of MHA.…”

“…EDOTEG synthesis was a two-step process according to our previous report . The synthesis started with the reaction between tosyl chloride and triethylene glycol monomethyl ether that gave the tosylate.…”

“…29,30 Recently, our group reported a sensor for biothiols based on a conductive polymer poly(EDOT-thioacetate-co-EDOT). 31 It was shown that the thioacetate moieties on the conducting polymer can be electrochemically converted into thiols, which can then reversibly form disulfide bonds upon electrochemical oxidation. In a subsequent work, we utilized a similar conducting polymer, coated onto an electrospun and micromachined substrate to afford an electrochemically switchable interface for the controlled capture and release of human cells.…”

Development of Highly Sensitive and Humidity Independent Room Temeprature NO2 Gas Sensor Using Two Dimensional Ti3C2Tx Nanosheets and One Dimensional WO3 Nanorods Nanocomposite

“…The main prerequisite for a typical room temperature sensor are high sensitivity and selectivity, fast response/recovery time, low detection limit, and reversibility even under high humidity and low temperature. Different materials including organic molecules, conducting polymer, metal oxides, two dimensional carbonaceous materials, − and transition metal dichalcogenides/carbide , have been explored in gas sensing applications. Among these materials, metal oxides have been widely investigated for monitoring different toxic gases due to their outstanding properties such as wide band gap, intrinsic oxygen vacancies, and high chemical, as well as thermal stability and an environmental friendly nature .…”