A novel electrochemical DNA biosensor for Ebola virus detection

Ilkhani, Hoda; Farhad, Siamak

doi:10.1016/j.ab.2018.06.010

Cited by 111 publications

(61 citation statements)

References 31 publications

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“…Recently, a novel electrochemical based biosensor for the detection of deadly EV-DNA had been successfully developed ( Ilkhani and Farhad, 2018 ). In this work, the DNA biosensor was fabricated using a thiolated DNA capture probe sequence immobilized onto the Au surface of screen-printed electrodes functionalized with thiolated single strand capture probe DNA (1 μM).…”

Section: Electrochemical Biosensors For Virus Detectionmentioning

confidence: 99%

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Khan¹,

Hasan

Hossain

et al. 2020

Biosensors and Bioelectronics

174

108

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Last few decades, viruses are a real menace to human safety. Therefore, the rapid identification of viruses should be one of the best ways to prevent an outbreak and important implications for medical healthcare. The recent outbreak of coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus which belongs to the single-stranded, positive-strand RNA viruses. The pandemic dimension spread of COVID-19 poses a severe threat to the health and lives of seven billion people worldwide. There is a growing urgency worldwide to establish a point-of-care device for the rapid detection of COVID-19 to prevent subsequent secondary spread. Therefore, the need for sensitive, selective, and rapid diagnostic devices plays a vital role in selecting appropriate treatments and to prevent the epidemics. During the last decade, electrochemical biosensors have emerged as reliable analytical devices and represent a new promising tool for the detection of different pathogenic viruses. This review summarizes the state of the art of different virus detection with currently available electrochemical detection methods. Moreover, this review discusses different fabrication techniques, detection principles, and applications of various virus biosensors. Future research also looks at the use of electrochemical biosensors regarding a potential detection kit for the rapid identification of the COVID-19.

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Section: Electrochemical Biosensors For Virus Detectionmentioning

confidence: 99%

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Khan¹,

Hasan

Hossain

et al. 2020

Biosensors and Bioelectronics

174

108

View full text Add to dashboard Cite

show abstract

“… Electrochemical format Electrochemical format Genosensors R1: 32 pmol L-1 Genosensors R2: 9 pmol L-1 Genosensors R1: 0.7 pmol L-1 Genosensors R2: 3 pmol L-1 [77] Zika virus Chikungunya viruses -a Chikungunya viruses -b Multiplex RT-qPCR 100 copies 5 copies 50 copies [78] Porcine epidemic diarrhea virus Immuno-chromatographic strip 1:50 [79] H1N1 of influenza A virus H3N2 of influenza A virus CdSe/CdS/ZnS quantum dot-linked rapid fluorescent immunochromatographic test 28.37 34.48 [80] Epstein-Barr virus Electrochemical detection 0.46 fM [81] Pseudorabies virus Porcine circovirus 3 SYBR green I-based duplex real-time PCR 37.8 copies/μL, 30.6 copies/μL [82] Classic swine fever virus Porcine circovirus 3 SYBR green I-based duplex real-time fluorescence quantitative PCR 23 copies/μL 36 copies/μL [83] Ebola virus Rolling circle amplification of Ebola virus and fluorescence detection based on graphene oxide 1.4 pM. [84] Ebola virus Microfluidic sample preparation multiplex 0.021 pfu/mL [85] Ebola virus Electrochemical DNA biosensor 4.7nm [86] Ebola virus RT-PCR 10 molecules/μl …”

Section: Current Scenario In the Detection Of Virusesmentioning

confidence: 99%

Electrochemical investigations for COVID-19 detection-A comparison with other viral detection methods

Bukkitgar

Shetti

Aminabhavi³

2021

Chemical Engineering Journal

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“…36 For instance, a novel electrochemical-based nucleic acid sensor was fabricated for Ebola virus RNA detection (Figure 1B). 25 In the first step, the gold surface of screen-printed electrode was functionalized with a thiolated DNA capture probe sequence via S-Au bonding. Then, biotinylated target strand DNA was immobilized to the electrode surface for hybridization of thiolated DNA single strand capture.…”

Section: Nucleic Acid-based Sensorsmentioning

confidence: 99%

Review—Chemical and Biological Sensors for Viral Detection

Özer

Geiss

Henry

2019

J. Electrochem. Soc.

128

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Infectious diseases commonly occur in contaminated water, food, and bodily fluids and spread rapidly, resulting in death of humans and animals worldwide. Among infectious agents, viruses pose a serious threat to public health and global economy because they are often difficult to detect and their infections are hard to treat. Since it is crucial to develop rapid, accurate, cost-effective, and in-situ methods for early detection viruses, a variety of sensors have been reported so far. This review provides an overview of the recent developments in electrochemical sensors and biosensors for detecting viruses and use of these sensors on environmental, clinical and food monitoring. Electrochemical biosensors for determining viruses are divided into four main groups including nucleic acid-based, antibody-based, aptamer-based and antigen-based electrochemical biosensors. Finally, the drawbacks and advantages of each type of sensors are identified and discussed.

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A novel electrochemical DNA biosensor for Ebola virus detection

Cited by 111 publications

References 31 publications

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Ultrasensitive detection of pathogenic viruses with electrochemical biosensor: State of the art

Electrochemical investigations for COVID-19 detection-A comparison with other viral detection methods

Review—Chemical and Biological Sensors for Viral Detection

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