A novel electrochemical hydrogen peroxide biosensor based on hemoglobin capped gold nanoclusters–chitosan composite

Shamsipur, Mojtaba; Pashabadi, Afshin; Molaabasi, Fatemeh

doi:10.1039/c5ra09216g

Cited by 24 publications

(15 citation statements)

References 60 publications

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“…Therefore, it is of high importance to be able to achieve sensitive determination of H 2 O 2 presence in many biological processes and related applications. Additionally, it is also well known that, due to its intrinsic peroxidase activity, Hb is an excellent protein to fabricate H 2 O 2 electrochemical biosensors (see, for example, works by Chen et al [ 20 ] or Shamsipur et al [ 21 ]).…”

Section: Introductionmentioning

confidence: 99%

Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance

et al. 2017

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In the present work, a nanostructured ZnO layer was synthesized onto a Metglas magnetoelastic ribbon to immobilize hemoglobin (Hb) on it and study the Hb’s electrochemical behavior towards hydrogen peroxide. Hb oxidation by H2O2 was monitored simultaneously by two different techniques: Cyclic Voltammetry (CV) and Magnetoelastic Resonance (MR). The Metglas/ZnO/Hb system was simultaneously used as a working electrode for the CV scans and as a magnetoelastic sensor excited by external coils, which drive it to resonance and interrogate it. The ZnO nanoparticles for the ZnO layer were grown hydrothermally and fully characterized by X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and photoluminescence (PL). Additionally, the ZnO layer’s elastic modulus was measured using a new method, which makes use of the Metglas substrate. For the detection experiments, the electrochemical cell was performed with a glass vial, where the three electrodes (working, counter and reference) were immersed into PBS (Phosphate Buffer Solution) solution and small H2O2 drops were added, one at a time. CV scans were taken every 30 s and 5 min after the addition of each drop and meanwhile a magnetoelastic measurement was taken by the external coils. The CV plots reveal direct electrochemical behavior of Hb and display good electrocatalytic response to the reduction of H2O2. The measured catalysis currents increase linearly with the H2O2 concentration in a wide range of 25–350 μM with a correlation coefficient 0.99. The detection limit is 25–50 μM. Moreover, the Metglas/ZnO/Hb electrode displays rapid response (30 s) to H2O2, and exhibits good stability and reproducibility of the measurements. On the other hand, the magnetoelastic measurements show a small linear mass increase versus the H2O2 concentration with a slope of 152 ng/μM, which is probably due to H2O2 adsorption in ZnO during the electrochemical reaction. No such effects were detected during the control experiment when only PBS solution was present for a long time.

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Section: Introductionmentioning

confidence: 99%

Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance

et al. 2017

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“…In this study, we synthesized random arrays of manganese-nanobar film at the surface of an Au electrode. In recent years, our research groups have been successfully report some application of nanomaterials in electrochemical sensors and biosensors [25][26][27][28]. The prepared electrode showed a distinct behavior at different AA centration ranges in a way that provides two paths for oxidation of AA through a direct path and an electrocatalysis path.…”

mentioning

confidence: 99%

A Dense Manganese Porphyrin-Nanobar Random Arrays: Direct and Electrocatalytic Oxidation of Ascorbic Acid in the Presence of Dopamine and Uric Acid

Shamsipur¹,

Hashem²,

Pashabadi³

2017

Int J Nanoparticles Nanotech

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Microelectrode arrays demonstrated an attractive behavior relate to macroelectrodes, as they can produce voltammetric responses of similar magnitude to their macro counterparts, but with a considerably less capacitive/background current. Hence, they have found an increasing use in electroanalytical sensing devices. Recently, some researches were focused on designing new types of sensing-arrays that employed micro/nanoelectrodes in both regular and random distributions [1,2]. These structures, due to their many advantageous properties, including low background charging currents, large current density related to convergent diffusion and importantly small diffu

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“…Therefore, despite the numerous methods for H 2 O 2 detection available, it is still of interest to develop a simple and direct method, that would be reliable, free from interferences and of lower cost. In this sense, electroanalytical methods have been used for the sensitive and selective determination of H 2 O 2 [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Hemin-Modified SnO2/Metglas Electrodes for the Simultaneous Electrochemical and Magnetoelastic Sensing of H2O2

et al. 2018

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In this work, we present a simple and efficient method for the preparation of hemin-modified SnO2 films on Metglas ribbon substrates for the development of a sensitive magneto-electrochemical sensor for the determination of H2O2. The SnO2 films were prepared at low temperatures, using a simple hydrothermal method, compatible with the Metglas surface. The SnO2 film layer was fully characterized by X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), photoluminescence (PL) and Fourier Transform-Infrared spectroscopy (FT-IR). The properties of the films enable a high hemin loading to be achieved in a stable and functional way. The Hemin/SnO2-Metglas system was simultaneously used as a working electrode (WE) for cyclic voltammetry (CV) measurements and as a magnetoelastic sensor excited by external coils, which drive it to resonance and interrogate it. The CV scans reveal direct reduction and oxidation of the immobilized hemin, as well as good electrocatalytic response for the reduction of H2O2. In addition, the magnetoelastic resonance (MR) technique allows the detection of any mass change during the electroreduction of H2O2 by the immobilized hemin on the Metglas surface. The experimental results revealed a mass increase on the sensor during the redox reaction, which was calculated to be 767 ng/μM. This behavior was not detected during the control experiment, where only the NaH2PO4 solution was present. The following results also showed a sensitive electrochemical sensor response linearly proportional to the concentration of H2O2 in the range 1 × 10−6–72 × 10−6 M, with a correlation coefficient of 0.987 and detection limit of 1.6 × 10−7 M. Moreover, the Hemin/SnO2-Metglas displayed a rapid response (30 s) to H2O2 and exhibits good stability, reproducibility and selectivity.

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A novel electrochemical hydrogen peroxide biosensor based on hemoglobin capped gold nanoclusters–chitosan composite

Cited by 24 publications

References 60 publications

Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance

Nanostructured ZnO in a Metglas/ZnO/Hemoglobin Modified Electrode to Detect the Oxidation of the Hemoglobin Simultaneously by Cyclic Voltammetry and Magnetoelastic Resonance

A Dense Manganese Porphyrin-Nanobar Random Arrays: Direct and Electrocatalytic Oxidation of Ascorbic Acid in the Presence of Dopamine and Uric Acid

Hemin-Modified SnO2/Metglas Electrodes for the Simultaneous Electrochemical and Magnetoelastic Sensing of H2O2

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