A Novel Extensin Gene Encoding a Hydroxyproline-Rich Glycoprotein Requires Sucrose for Its Wound-Inducible Expression in Transgenic Plants

Ahn, Ji‐Hoon; Choi, Yeonhee; Kwon, Young Jun; Kim, Sang‐Gu; Choi, Yang Do; Lee, Jong Seob

doi:10.2307/3870244

Cited by 24 publications

(34 citation statements)

References 37 publications

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“…Our previous studies have shown that the SbHRGP3 gene encoding an extensin in soybean is expressed mainly in the region undergoing maturation in the primary and secondary roots and may be involved in the cessation of cell elongation in the region of maturation of the soybean roots (Ahn et al, 1996(Ahn et al, , 1998. In this study, we have identified a rootspecific expansin gene from soybean and examined its expression patterns and its role during root development in soybean and transgenic tobacco plants.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously shown that the SbHRGP3 gene of soybean is involved in the maturation event that leads not only to the cessation of cell elongation but also to the prevention of further elongation of soybean roots (Ahn et al, 1996(Ahn et al, , 1998. SbHRGP3 expression was specific to the epidermal cells of the region from which the secondary root was to be initiated in soybean and transgenic tobacco plants.…”

Section: Roles Of the Gmexp1 Gene During Root Development In Soybeanmentioning

confidence: 99%

“…Our previous studies have shown that expression of an extensin gene encoding a soybean (Glycine max) Hyp-rich glycoprotein, SbHRGP3, was up-regulated by the maturation of the primary root (Ahn et al, 1996). Its expression was also observed in the mature region of the secondary root, suggesting its role in the maturation of soybean roots by cessation of cell elongation in the region of maturation (Ahn et al, 1998).…”

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Expression of an Expansin Gene Is Correlated with Root Elongation in Soybean

et al. 2003

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Expansin is a family of proteins that catalyze long-term expansion of cell walls and has been considered a principal protein that affects cell expansion in plants. We have identified the first root-specific expansin gene in soybean (Glycine max), GmEXP1, which may be responsible for root elongation. Expression levels of GmEXP1 were very high in the roots of 1-to 5-d-old seedlings, in which rapid root elongation takes place. Furthermore, GmEXP1 mRNA was most abundant in the root tip region, where cell elongation occurs, but scarce in the region of maturation, where cell elongation ceases, implying that its expression is closely related to root development processes. In situ hybridization showed that GmEXP1 transcripts were preferentially present in the epidermal cells and underlying cell layers in the root tip of the primary and secondary roots. Ectopic expression of GmEXP1 accelerated the root growth of transgenic tobacco (Nicotiana tabacum) seedlings, and the roots showed insensitivity to obstacle-touching stress. These results imply that the GmEXP1 gene plays an important role in root development in soybean, especially in the elongation and/or initiation of the primary and secondary roots.The root is a plant organ that has adapted to acquire water and nutrients from the environment (Schiefelbein et al., 1997). The root system has recently been the focus of interest as a useful system for understanding organ development because it is a relatively simple organ, its growth pattern is uniform, and it has a small number of differentiated cell types (Aeschbacher et al., 1994). Furthermore, the development of new roots (secondary or lateral roots) from an existing root (primary root) provides a novel opportunity to investigate cellular differentiation and development in plants.Although the primary and secondary roots share many basic structural features, they are different in their origin (Scheres et al., 1996). A basic feature of the root is its radial pattern, which is made up of concentric layers of tissues. Three fundamental types of the tissues are the epidermis, the cortex, and the vascular tissues (Esau, 1977;Dolan et al., 1993;Raven et al., 1999). In the longitudinal section, the root can be divided into three different regions: those of cell division, elongation, and maturation (specialization) (Dolan et al., 1993;Baluska et al., 1996;Howell, 1998;Raven et al., 1999). The region of cell division contains the root apical meristem, which carries out new cell divisions but does not elongate newly divided cells immediately. The cells derived from the region of cell division expand and elongate mostly in the region of elongation. After they have elongated, the cells begin to differentiate in the region of maturation, where root hairs and the secondary roots are initiated. The events of cell elongation and maturation occurring in the root have been suggested to be controlled by the extensibility of the cell wall and the turgor pressure inside the cell (Cosgrove, 1996).It has been proposed that development of the s...

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Section: Discussionmentioning

confidence: 99%

Section: Roles Of the Gmexp1 Gene During Root Development In Soybeanmentioning

confidence: 99%

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confidence: 99%

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Expression of an Expansin Gene Is Correlated with Root Elongation in Soybean

et al. 2003

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“…For example, the tobacco HRGPnt3 gene is specifically expressed in a subset of the pericycle and endodermal cells from which a lateral root initiates (Keller and Lamb, 1989). A soybean (Glycine max) extensin gene, SbHRGP3, is expressed in hypocotyl and roots of seedlings (Ahn et al, 1996). This was shown by using an SbHRGP3 promoter-GUS chimeric gene that was expressed in the rhizodermal cells of the zone from which the lateral root was to be initiated.…”

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The Expression of an Extensin-Like Protein Correlates with Cellular Tip Growth in Tomato

et al. 2002

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“…Tobacco plants have also been used as a heterologous system to study the expression pattern of extensin genes isolated from species for which an efficient transformation method is lacking. For example, the expression of the extA::GUS, HRGP4.1::GUS, and the SbHRGP3::GUS transgenes were examined into tobacco plants, elucidating the spatial and temporal regulation of the oilseed rape extensin gene extA (Shirsat et al, 1991), the bean extensin gene HRGHP4.1 (Wycoff et al, 1995), and the soybean extensin gene SbHRGP (Ahn et al, 1996), respectively. There is no direct evidence concerning the role extensins play in the plant cell wall, although substantial indirect evidence suggests their involvement in plant development and also in defence.…”

Section: Introductionmentioning

confidence: 99%

Histochemical map of the ectopic expression of the Arabidopsis atExt1 extensin gene in transgenic tobacco

Merkouropoulos

Shirsat

2012

Genet. Mol. Res.

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ABSTRACT. Histochemical analysis of transgenic tobacco plants harboring the promoter of the Arabidopsis extensin gene atExt1 fused to the β-glucuronidase gene coding sequence demonstrated expression of the transgene in stem tissues. The transgene was expressed in cells of the internal and external phloem at the nodal and internodal regions of the older parts of mature stems. In younger parts of the same stem, expression of the transgene was slightly modified: expression was detected in the internal phloem in the internodal areas. In the nodal areas, the phloem tissue and the surrounding parenchyma were stained, demonstrating transgene expression. Expression was also seen in the phloem of the inflorescence; it was non-specific at the junctions of the flowers to the inflorescence. β-glucuronidase (reporter gene) staining was also observed in the pollen grains and at the base of the corolla.

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A Novel Extensin Gene Encoding a Hydroxyproline-Rich Glycoprotein Requires Sucrose for Its Wound-Inducible Expression in Transgenic Plants

Cited by 24 publications

References 37 publications

Expression of an Expansin Gene Is Correlated with Root Elongation in Soybean

Expression of an Expansin Gene Is Correlated with Root Elongation in Soybean

The Expression of an Extensin-Like Protein Correlates with Cellular Tip Growth in Tomato

Histochemical map of the ectopic expression of the Arabidopsis atExt1 extensin gene in transgenic tobacco

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