A Novel Extract From Ginkgo biloba Inhibits Neuroinflammation and Maintains White Matter Integrity in Experimental Stroke

Dong, Wen; Gong, Ting; Zhao, Shunying; Wen, Shaohong; Chen, Qingfang; Jiang, Mingyu; Ye, Weizhen; Qiu-ru, Huang; Wang, Chunjuan; Chao, Yang; Liu, Xiangrong; Wang, Yongjun

doi:10.1016/j.neuroscience.2023.05.015

Cited by 11 publications

(3 citation statements)

References 55 publications

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“…When exposed to LPS, microglia release inflammatory cytokines, leading to neuronal dysfunction in the brain and the development of neurodegenerative diseases [ 23 ]. Therefore, natural products that can inhibit the excessive production of inflammatory mediators by microglia may serve as potential therapeutic agents [ 24 ]. This study discovered that therapy with dihydropashanone greatly decreased the production of inflammatory components, including nitric oxide, in the damaged BV2 cells, and inhibited the expression of iNOS [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Dihydropashanone Isolated from Lindera erythrocarpa, a Potential Natural Product for the Treatment of Neurodegenerative Diseases

Liu,

Yoon,

Lee

et al. 2024

IJMS

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Lindera erythrocarpa, a flowering plant native to eastern Asia, has been reported to have neuroprotective activity. However, reports on the specific bioactive compounds in L. erythrocarpa are finite. The aim of this study was to investigate the anti-neuroinflammatory and neuroprotective effects of the compounds isolated from L. erythrocarpa. Dihydropashanone, a compound isolated from L. erythrocarpa extract, was found to have protected mouse hippocampus HT22 cells from glutamate-induced cell death. The antioxidant and anti-inflammatory properties of dihydropashanone in mouse microglial BV2 and HT22 cells were explored in this study. The results reveal that dihydropashanone inhibits lipopolysaccharide-induced inflammatory response and suppresses the activation of nuclear factor (NF)-κB in BV2 cells. In addition, dihydropashanone reduced the buildup of reactive oxygen species in HT22 cells and induced activation of the nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase (HO)-1 signaling pathway in BV2 and HT22 cells. Our results suggest that dihydropashanone reduces neuroinflammation by decreasing NF-κB activation in microglia cells and protects neurons from oxidative stress via the activation of the Nrf2/HO-1 pathway. Thus, our data suggest that dihydropashanone offers a broad range of applications in the treatment of neurodegenerative illnesses.

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Section: Discussionmentioning

confidence: 99%

Dihydropashanone Isolated from Lindera erythrocarpa, a Potential Natural Product for the Treatment of Neurodegenerative Diseases

Liu,

Yoon,

Lee

et al. 2024

IJMS

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“…Article Related Abbreviations: CCC, countercurrent chromatography; EBuWat, acetate/n-butanol/water; GBL, Ginkgo biloba L. leaves; HepEWat, n-heptane/ethyl acetate/water; HepEMWat, n-heptane/ethyl acetate/methanol/water; HPLC, high-performance liquid chromatography; HRMS, high-resolution mass spectra; TCM, traditional Chinese medicine; TFA, trifluoroacetic acid. biloba leaves (GBL) and its standardized extract formulation (EGb 761) has been extensively and widely employed in the prevention and treatment of various diseases, such as neurodegenerative and cardiovascular diseases, cancers, and tinnitus [3][4][5][6][7]. Flavonoids, terpenoids, ginkgolic acids, and polysaccharides constitute the main components of GBL [8,9].…”

Section: Introductionmentioning

confidence: 99%

Preparative separation of the constituents from Ginkgo biloba L. leaves by a combination of multi‐stage solvent extraction and countercurrent chromatography

Liu,

Tian,

et al. 2024

J of Separation Science

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In this study, we employed a combination approach for the preparative separation of constituents from Ginkgo biloba L. leaves. It involved multi‐stage solvent extractions utilizing two‐phase multi‐solvent systems and countercurrent chromatography (CCC) separations using three different solvent systems. The n‐heptane/ethyl acetate/water (1:1:2, v/v) and n‐heptane/ethyl acetate/methanol/water (HepEMWat, 7:3:7:3, v/v) solvent systems were screened out as extraction systems. The polarities of the upper and lower phases in the multi‐solvent systems were adjustable, enabling the effectively segmented separation of complex constituents in G. biloba L. The segmented products were subsequently directly utilized as samples and separated using CCC with the solvent systems acetate/n‐butanol/water (4:1:5, v/v), HepEMWat (5:5:5:5, v/v), and HepEMWat (9:1:9:1, v/v), respectively. As a result, a total of 11 compounds were successfully isolated and identified from a 2 g methanol extract of G. biloba L through two‐stage extraction and three CCC separation processes; among them, nine compounds exhibited high‐performance liquid chromatography purity exceeding 85%.

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“…Particularly, G. biloba L. extract (GBE) appeared at the historic moment when scholars were pursuing more active compounds to improve pharmacological activity. In previous studies, a series of preclinical or clinical research shows clearly that GBE has many positive effects including scavenging radicals; antitumor, anti‐oxidation, and therapeutic effects for cerebral and peripheral vascular diseases; and protective effects in the central nervous system such as dementia and Alzheimer's disease 7–9 . In addition, GBE has also been used as raw material to prepare various preparations, such as Ginaton injection and Ginaton tablets, which have been commonly used for stroke treatment.…”

Section: Introductionmentioning

confidence: 99%

Study on network pharmacology of Ginkgo biloba extract against ischaemic stroke mechanism and establishment of UPLC‐MS/MS methods for simultaneous determination of 19 main active components

Yin,

Lian,

et al. 2023

Phytochemical Analysis

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IntroductionGinkgo biloba extract (GBE) is an effective substance from traditional Chinese medicine (TCM) G. biloba for treating ischaemic stroke (IS). However, its active ingredients and mechanism of action remain unclear.ObjectivesThis study aimed to reveal the potential active component group and possible anti‐IS mechanism of GBE.Materials and methodsThe network pharmacology method was used to reveal the possible anti‐IS mechanism of these active ingredients in GBE. An ultra‐high‐performance liquid chromatography triple quadrupole electrospray tandem mass spectrometry (UPLC‐MS/MS) method was established for the simultaneous detection of the active ingredients of GBE.ResultsThe active components of GBE anti‐IS were screened by literature integration. Network pharmacology results showed that the anti‐IS effect of GBE is achieved through key active components such as protocatechuic acid, bilobalide, ginkgolide A, and so on. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the possible anti‐IS mechanism of GBE is regulating the PI3K‐Akt signalling pathway and other signal pathways closely related to inflammatory response and apoptosis regulation combined with AKT1, MAPK, TNF, ALB, CASP3, and other protein targets. Nineteen main constituents in seven batches of GBE were successfully analysed using the established UPLC‐MS/MS method, and the results showed that the content of protocatechuic acid, gallic acid, ginkgolide A, and so forth was relatively high, which was consistent with network pharmacology results, indicating that these ingredients may be the key active anti‐IS ingredients of GBE.ConclusionThis study revealed the key active components and the anti‐IS mechanism of GBE. It also provided a simple and sensitive method for the quality control of related preparations.

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A Novel Extract From Ginkgo biloba Inhibits Neuroinflammation and Maintains White Matter Integrity in Experimental Stroke

Cited by 11 publications

References 55 publications

Dihydropashanone Isolated from Lindera erythrocarpa, a Potential Natural Product for the Treatment of Neurodegenerative Diseases

Dihydropashanone Isolated from Lindera erythrocarpa, a Potential Natural Product for the Treatment of Neurodegenerative Diseases

Preparative separation of the constituents from Ginkgo biloba L. leaves by a combination of multi‐stage solvent extraction and countercurrent chromatography

Study on network pharmacology of Ginkgo biloba extract against ischaemic stroke mechanism and establishment of UPLC‐MS/MS methods for simultaneous determination of 19 main active components

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