A novel feeding strategy during the production phase for enhancing the enzymatic synthesis of S-adenosyl-l-methionine by methylotrophic Pichia pastoris

Enhancing yield of S-adenosylmethionine in Pichia pastoris by controlling NH4 + concentration

“…The novel feature of SAM accumulation in P. pastoris fermentation of NH 4 + concentration controlled was reduction of process of SAM accumulation as the result of enhancing yield of SAM. Forty-three hours were saved in our experiment compared with present report [6].…”

“…6 Ammonium hydroxide and potassium hydroxide were used to adjust pH to 5.0 for different ammonium concentrations. Ammonium sulfate was added when high NH 4 + concentration was needed.…”

“…6 [3]. In recent years, many researchers have tried to prepare SAM commercially in P. pastoris by genetic manipulation or cultivation strategies [4][5][6]. In these reports, almost 4 days of induction was needed to accumulate SAM.…”

Co-production of S-adenosyl-L-methionine and L-isoleucine in Corynebacterium glutamicum

“…The level of by-product L-lysine in IWJ001/pDXW-8-metk and IWJ001/pDXW-8-metk-vgb also decreased. The competition for ATP between SAM and L-isoleucine might exist in IWJ001/pDXW-8-metk and IWJ001/pDXW-8-metk-vgb [40][41].…”

Enzymatic Synthesis of S-Adenosylmethionine Using Immobilized Methionine Adenosyltransferase Variants on the 50-mM Scale

“…Most studies have been focused on the improvement of SAM production by altering Saccharomyces cerevisiae or Pichia pastoris strains by a genetic engineering approach or by optimization of culture conditions [9][10][11][12][13][14][15][16]. However, the production of SAM by yeast fermentation has some obvious drawbacks, such as a long fermentation period, low SAM content and yield, and low conversion rate of the substrate.…”