A novel glaucoma approach: Stem cell regeneration of the trabecular meshwork

Coulon, Sara J; Schuman, Joel S.; Du, Yiqin; Fard, Mohammad Reza Bahrani; Ethier, C. Ross; Stamer, W. Daniel

doi:10.1016/j.preteyeres.2022.101063

Cited by 27 publications

(25 citation statements)

References 114 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, we used a simple elastic material model with an idealized IOP load boundary that may not fully represent the dynamic biomechanical behavior seen with a physiologic load boundary. While great strides have been made in understanding the mechanisms that regulate aqueous outflow resistance in the conventional outflow pathway [ 3 , 4 , 28 , 58 , 61 , 62 , 63 , 64 , 65 ], the mechanism of outflow resistance regulation with a dynamic physiologic IOP load boundary is largely unknown. Although it has been shown experimentally [ 38 , 66 ] and numerically [ 60 ] that increased TM stiffness causes increased outflow resistance, it remains unclear how viscoelastic outflow tissues with a physiologic IOP load boundary may affect outflow resistance.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Intraocular Pressure Load Boundary on the Biomechanics of the Human Conventional Aqueous Outflow Pathway

et al. 2022

View full text Add to dashboard Cite

Background: Aqueous humor outflow resistance in the trabecular meshwork (TM), juxtacanalicular connective tissue (JCT), and Schlemm’s canal (SC) endothelium of the conventional outflow pathway actively contribute to intraocular pressure (IOP) regulation. Outflow resistance is actively affected by the dynamic outflow pressure gradient across the TM, JCT, and SC inner wall tissues. The resistance effect implies the presence of a fluid–structure interaction (FSI) coupling between the outflow tissues and the aqueous humor. However, the biomechanical interactions between viscoelastic outflow tissues and aqueous humor dynamics are largely unknown. Methods: A 3D microstructural finite element (FE) model of a healthy human eye TM/JCT/SC complex was constructed with elastic and viscoelastic material properties for the bulk extracellular matrix and embedded elastic cable elements. The FE models were subjected to both idealized and a physiologic IOP load boundary using the FSI method. Results: The elastic material model for both the idealized and physiologic IOP load boundary at equal IOPs showed similar stresses and strains in the outflow tissues as well as pressure in the aqueous humor. However, outflow tissues with viscoelastic material properties were sensitive to the IOP load rate, resulting in different mechanical and hydrodynamic responses in the tissues and aqueous humor. Conclusions: Transient IOP fluctuations may cause a relatively large IOP difference of ~20 mmHg in a very short time frame of ~0.1 s, resulting in a rate stiffening in the outflow tissues. Rate stiffening reduces strains and causes a rate-dependent pressure gradient across the outflow tissues. Thus, the results suggest it is necessary to use a viscoelastic material model in outflow tissues that includes the important role of IOP load rate.

show abstract

Section: Introductionmentioning

confidence: 99%

The Effect of Intraocular Pressure Load Boundary on the Biomechanics of the Human Conventional Aqueous Outflow Pathway

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Notably, iPSCs still received attention in 2022 ( Coulon et al, 2022 ). The trabecular meshwork cells can mediate the regulation of intraocular pressure by regulating aqueous humor flow ( Zhu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

A bibliometric analysis of the application of stem cells in glaucoma research from 1999 to 2022

Tao

Zhang

Mao

et al. 2023

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Background: Glaucoma, a neurodegenerative disease of the retina, is the leading cause of irreversible blindness. Stem cells have therapeutic potential for glaucoma. However, few bibliometric studies have been published in this field. Concerning a visual map, this article aims to characterize the research context, cooperation relationship, hotspots, and trends concerning the application of stem cells in glaucoma research.Methods: Publications focusing on stem cell research and glaucoma were retrieved from the Web of Science Core Collection. VOSviewer, CiteSpace, Microsoft Excel, and Scimago Graphica were used to map the contributions of countries or regions, authors, organizations, and journals. Journal Impact Factor data were obtained from the Web of Science Core Collection. We analyzed the tendencies, hotspots, and knowledge networks using VOSviewer, and CiteSpace.Results: We analyzed 518 articles published from 1999 through 2022. In the first decade, the number of articles in this field increased slowly, and there was a marked acceleration in publication frequency after 2010. The United States, China, and England were the main contributors. Yiqin Du was the most prolific author, and among the top 10 prolific writers, Keith R. Martin’s work was cited most frequently. Investigative Ophthalmology and Visual Science, Experimental Eye Research, and Cornea published the most articles in this domain. The three most commonly co-cited journals were Investigative Ophthalmology and Visual Science, Experimental Eye Research, and Proceedings of the National Academy of Sciences of the United States of America. The Central South University, the University of Pittsburgh, and the National Institutes of Health National Eye Institute were highly prolific institutions in this research area. Our keywords analysis with VOSviewer suggested directions of future research and yielded the following recent key themes, extracellular vesicles, exosomes, mitochondria, growth factors, oxidative stress, and ocular diseases. Four co-cited references had a citation burst duration until 2022.Conclusion: With improvements in overall quality of life and demographic transitions toward population aging, research and clinical focus on eye care has increased, with glaucoma as a key area of emphasis. This study added to our understanding of the global landscape and Frontier hotspots in this field.

show abstract

“…41,42 Accumulated evidence suggests the existence of TM stem cells (TMSCs) in Schwalbe's line, also known as the insert or the non-filtering region of TM, based on the high percentage of cell division after injury and the expression of stem cell markers ABCG2, p75, and Oct4 in this area. 43,44 Therefore, the TM probably shares the same stem cell origin with the corneal endothelium. TMSCs have been isolated from Schwalbe's line and TM tissue and enriched using fluorescence-activated cell sorting or clonal culture.…”

Section: Trabecular Meshworkmentioning

confidence: 99%

Adult stem cells in the eye: Identification, characterisation, and therapeutic application in ocular regeneration – A review

Xiao,

McGhee,

Zhang

2024

Clinical Exper Ophthalmology

View full text Add to dashboard Cite

Adult stem cells, present in various parts of the human body, are undifferentiated cells that can proliferate and differentiate to replace dying cells within tissues. Stem cells have specifically been identified in the cornea, trabecular meshwork, crystalline lens, iris, ciliary body, retina, choroid, sclera, conjunctiva, eyelid, lacrimal gland, and orbital fat. The identification of ocular stem cells broadens the potential therapeutic strategies for untreatable eye diseases. Currently, stem cell transplantation for corneal and conjunctival diseases remains the most common stem cell‐based therapy in ocular clinical management. Lens epithelial stem cells have been applied in the treatment of paediatric cataracts. Several early‐phase clinical trials for corneal and retinal regeneration using ocular stem cells are also underway. Extensive preclinical studies using ocular stem cells have been conducted, showing encouraging outcomes. Ocular stem cells currently demonstrate great promise in potential treatments of eye diseases. In this review, we focus on the identification, characterisation, and therapeutic application of adult stem cells in the eye.

show abstract

A novel glaucoma approach: Stem cell regeneration of the trabecular meshwork

Cited by 27 publications

References 114 publications

The Effect of Intraocular Pressure Load Boundary on the Biomechanics of the Human Conventional Aqueous Outflow Pathway

The Effect of Intraocular Pressure Load Boundary on the Biomechanics of the Human Conventional Aqueous Outflow Pathway

A bibliometric analysis of the application of stem cells in glaucoma research from 1999 to 2022

Adult stem cells in the eye: Identification, characterisation, and therapeutic application in ocular regeneration – A review

Contact Info

Product

Resources

About