A Novel High Discriminatory Protocol for the Detection of Borrelia afzelii, Borrelia burgdorferi Sensu Stricto and Borrelia garinii in Ticks

Chiappa, Giulia; Perini, Matteo; Cafiso, Alessandra; Nodari, Riccardo; Wilhelmsson, Peter; Lindgren, Per-Eric; Omazic, Anna; Ullman, Karin; Moutailler, Sara; Kjellander, Petter; Bazzocchi, Chiara; Grandi, Giulio

doi:10.3390/pathogens11111234

Cited by 3 publications

(1 citation statement)

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“…Indeed, when focusing on the regional genospecies differences and concerning the genome of the complex Bb sl, it has been shown that even if the linear chromosome of 910 kilobases is highly conserved among the B LG, the plasmids (9 linear plasmids and 12 circular plasmids) [ 10 ] show a high degree of variation [ 9 , 11 , 12 ]. Some researchers have shown, for example by studying OspC (Outer Surface Proteins) typing or by multilocus sequence analysis (MLSA), many differences between genospecies in Europe and the United States [ 13 – 15 ]. Intraspecies diversity has also been noted, in the US, where patients from New York and Wisconsin were infected by two distinct populations of Bb ss but genetically and phylogenetically closely related [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Lyme borreliosis diagnosis: state of the art of improvements and innovations

Guérin,

Shawky,

Zedan

et al. 2023

BMC Microbiol

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With almost 700 000 estimated cases each year in the United States and Europe, Lyme borreliosis (LB), also called Lyme disease, is the most common tick-borne illness in the world. Transmitted by ticks of the genus Ixodes and caused by bacteria Borrelia burgdorferi sensu lato, LB occurs with various symptoms, such as erythema migrans, which is characteristic, whereas others involve blurred clinical features such as fatigue, headaches, arthralgia, and myalgia. The diagnosis of Lyme borreliosis, based on a standard two-tiered serology, is the subject of many debates and controversies, since it relies on an indirect approach which suffers from a low sensitivity depending on the stage of the disease. Above all, early detection of the disease raises some issues. Inappropriate diagnosis of Lyme borreliosis leads to therapeutic wandering, inducing potential chronic infection with a strong antibody response that fails to clear the infection. Early and proper detection of Lyme disease is essential to propose an adequate treatment to patients and avoid the persistence of the pathogen. This review presents the available tests, with an emphasis on the improvements of the current diagnosis, the innovative methods and ideas which, ultimately, will allow more precise detection of LB.

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Section: Introductionmentioning

confidence: 99%

Lyme borreliosis diagnosis: state of the art of improvements and innovations

Guérin,

Shawky,

Zedan

et al. 2023

BMC Microbiol

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Реактивація Хронічного Лайм-Бореліозу Після Перенесеної Інфекції Covid-19: Клінічний Випадок

Господарський,

Бойко

2024

ЗКЕМ

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РЕЗЮМЕ. Враховуючи зростаючу актуальність комбінованого перебігу інфекційних захворювань розглянуто клінічний випадок реактивації хронічного Лайм-бореліозу після COVID-19. Мета роботи – підтвердити потенційний вплив коронавірусної хвороби на можливість реактивації хронічної інфекційної патології, навіть за умови легкого перебігу COVID-19 на прикладі клінічного випадку реактивації хронічного Лайм-бореліозу. Матеріал і методи. При дослідженні застосовані клініко-анамнестичні, епідеміологічні, лабораторні (загальноклінічні, лабораторні та інструментальні (електрокардіографія, Холтерівське моніторування, рентгенологічне) обстеження. Результати. Був встановлений діагноз бореліозного поліартриту, а за результатами лабораторного та інструментального обстеження – Лайм-міокардиту. У ході подальшого пошуку потенційного збудника, який призвів до виявлених змін, хворий протестований на виявлення антитіл до Bоrrelia burgdorferi методом імуноферментного аналізу. Отриманий позитивний результат при детекції специфічних антитіл (IgM – 46,64 ОД/мл, IgG – 87,31 ОД/мл) вказував на реактивацію Лайм-бореліозу. Призначено лікування: доксициклін 100 мг × 2 рази/добу на 28 днів, протизапальна терапія. Після завершення курсу етіотропної терапії – клінічна ремісія, а також – негативні результати специфічних IgM через 3, 6 і 12 місяців. Висновок. На клінічному випадку показані труднощі встановлення діагнозу реактивації Лайм-бореліозу, наголошено на необхідності клінічної настороженості фахівців практичної охорони здоров’я щодо подібних випадків, навіть за умови легкого перебігу COVID-19.

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Ticks - public health risks in urban green spaces

Janzén,

Choudhury,

Hammer

et al. 2024

BMC Public Health

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Background Urban green spaces are important for human health, but they may expose visitors to tick-borne diseases. This not only presents a potential public health challenge but also undermines the expected public health gains from urban green spaces. The aim of this study is to assess the public health risk of tick-borne diseases in an urban green space used for recreation in Stockholm, Sweden. Methods We used a mixed method approach identifying both the magnitude of the tick hazard and the extent of the human exposure to tick-borne diseases. At six entry points to an urban green space, we sampled ticks and documented microhabitat conditions from five randomly assigned 2 m × 2 m plots. Surrounding habitat data was analyzed using geographical information system (GIS). Nymphs and adult ticks were tested for Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum using TaqMan qPCR. Positive B. burgdorferi (s.l.) ticks were further analyzed by nested PCR amplification and sequence analysis. Population census data and visitor count data were used to estimate the degree of human exposure to tick-borne diseases. To further understand the degree to which visitors get in contact with infected ticks we also conducted interviews with visitors to green spaces. Results High tick densities were commonly found in humid broadleaved forest with low field vegetation. High pathogen prevalence was significantly correlated with increasing proportions of artificial areas. Integrating the tick hazard with human exposure we found that the public health risk of tick-borne diseases was moderate to high at most of the studied entry points. Many of the visitors frequently used urban green spaces. Walking was the most common activity, but visitors also engaged in activities with higher risk for tick encounters. Individual protective measures were connected to specific recreational activities such as picking berries or mushrooms. Conclusions The number of visitors can be combined with tick inventory data and molecular analyses of pathogen prevalence to make crude estimations of the public health risk of tick-borne diseases in urban green spaces. The risk of encountering infected ticks is omnipresent during recreational activities in urban green spaces, highlighting the need for public health campaigns to reduce the risk of tick-borne diseases.

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A Novel High Discriminatory Protocol for the Detection of Borrelia afzelii, Borrelia burgdorferi Sensu Stricto and Borrelia garinii in Ticks

Cited by 3 publications

References 50 publications

Lyme borreliosis diagnosis: state of the art of improvements and innovations

Lyme borreliosis diagnosis: state of the art of improvements and innovations

Реактивація Хронічного Лайм-Бореліозу Після Перенесеної Інфекції Covid-19: Клінічний Випадок

Ticks - public health risks in urban green spaces

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