A Novel High Resolution In Vivo Digital Imaging System for the Evaluation of Experimental Cataract in Diabetic Rats

Kametaka, Sokichi; Kasahara, Tomoyuki; Ueo, Mayumi; Takenaka, Mariko; Saito, Maki; Sakamoto, Kenji; Nakahara, Tsutomu; Ishii, Kunio

doi:10.1254/jphs.fp0071500

Cited by 13 publications

(19 citation statements)

References 18 publications

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“…images of the whole lens of small animals and showed that the combination of streptozotocin treatment and D-glucose feeding was a useful procedure to minimize the variability and to shorten the period required for the development of diabetic cataract in rats (32). Using the digital camera system and the procedure, we found that nifedipine significantly slows the progression, but not the onset, of diabetic cataracts.…”

Section: Discussionmentioning

confidence: 90%

“…Using the non-invasive and repeatable in vivo digital camera system designed for capturing full lens images of small animals, we assessed the effect of nifedipine and verapamil on the onset and progression of diabetic cataracts by comparing the severity of cataracts with two methods based on different principles (32). This work has been partly presented at the 114th Kanto Area Regional Meeting of The Japanese Pharmacological Society.…”

Section: +mentioning

confidence: 99%

“…Induction of diabetes was confirmed with high plasma glucose levels (>350 mg / dl) on the third day after streptozotocin injection. Rats were given drinking water containing 5% D-glucose following treatment with streptozotocin to minimize the variability and to shorten the term in the development of diabetic cataracts by keeping extremely high plasma glucose levels (32). Plasma glucose levels were determined with a commercially available kit (Glucose Test Wako; Wako Pure Chemical, Osaka).…”

Section: Animals and Induction Of Diabetesmentioning

confidence: 99%

“…The progression of a cataract was assessed on a weekly basis as described previously (32). In brief, the high-resolution full lens images in the horizontal plane were captured with the digital camera system equipped with the non-reflecting illuminator.…”

Section: Evaluation Of Cataractsmentioning

confidence: 99%

“…In brief, the high-resolution full lens images in the horizontal plane were captured with the digital camera system equipped with the non-reflecting illuminator. Using the digital images of lenses, the severity of diabetic cataract was assessed by an observer-based scoring method and by a quantitative image-analysis method (32).…”

Section: Evaluation Of Cataractsmentioning

confidence: 99%

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Effect of Nifedipine on Severe Experimental Cataract in Diabetic Rats

et al. 2008

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Abstract. We examined the effects of Ca 2+-channel blockers on sugar cataract formation in streptozotocin (65 mg / kg, i.v.)-induced diabetic rats that were given 5% D-glucose as drinking water. The diabetic rats were treated with an L-type Ca 2+-channel blocker, nifedipine or verapamil, for 9 weeks from the 3rd day of streptozotocin injection. Using the full lens images of the horizontal plane captured with the new digital camera system that we developed recently, the cataract formation was quantitatively assessed in parallel with the conventional scaling method. In the animal model of diabetes mellitus, the cataracts at the peripheral region of the lens were detected 2 weeks after induction of hyperglycemia and progressed depending on the length of the diabetic period. The majority of them developed mature cataracts after 9 weeks of hyperglycemia. Nifedipine slowed the progression rate of diabetic cataracts without affecting the period of time required for the onset of this disease, whereas verapamil had no significant inhibitory effect on the diabetic cataract. These findings suggest that nifedipine may be considered as a candidate drug to suppress the progression of diabetic cataracts.

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Section: Discussionmentioning

confidence: 90%

Section: +mentioning

confidence: 99%

Section: Animals and Induction Of Diabetesmentioning

confidence: 99%

Section: Evaluation Of Cataractsmentioning

confidence: 99%

Section: Evaluation Of Cataractsmentioning

confidence: 99%

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Effect of Nifedipine on Severe Experimental Cataract in Diabetic Rats

et al. 2008

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Ferulic Acid Protects Human Lens Epithelial Cells Against UVA‐Induced Oxidative Damage by Downregulating the DNA Demethylation of the Keap1 Promoter

Ling,

Zhu,

Yan

et al. 2024

J Biochem & Molecular Tox

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Ultraviolet (UV) radiation‐triggered production of reactive oxygen species (ROS) is a primary contributor to apoptosis in human lens epithelial cells (HLECs), which can ultimately result in cataract formation. The nuclear factor erythroid‐2‐related factor 2 (Nrf2)‐Kelch ECH associating protein 1 (Keap1) pathway, a fundamental oxidative stress regulation mechanism, plays a crucial role in the development of cataracts. Ferulic acid (FA), recognized for its potent antioxidant properties can activate the Nrf2 signaling pathway to mitigate oxidative damage and cell apoptosis. In this study, we have demonstrated the protective effects of FA in reducing UVA‐induced oxidative damage and apoptosis in HLECs through the modulation of the Keap1/Nrf2 pathway, as evidenced by both cellular and animal experiments. HLECs and Lens were exposed to 10 J/cm2 UVA radiation with or without prior treatment with FA. We found that UVA radiation increased oxidative damage and cell apoptosis in HLECs, ultimately leading to opacification of rat lenses, while FA was able to attenuate both oxidative damage and cell apoptosis in HLECs and reduce the degree of lens opacification. FA upregulated the expression of antioxidant response factors of the Keap1/Nrf2 pathway and downregulated the expression of apoptosis‐related genes in HLECs, as demonstrated by Western blot and RT‐qPCR analyses. We also found that UVA radiation increased the degree of demethylation of the Keap1 promoter in HLECs, whereas FA reduced the level of Keap1 promoter demethylation as determined by DNA sequencing. Additionally, UVA upregulated the expression of DNA active demethylase of the Keap1 promoter in HLECs, Dnmt1, Dnmt3a, and Dnmt3b, as shown by immunofluorescence, Western blot, and RT‐qPCR, however, FA attenuated the activity of the passive demethylase TET1 in addition to the active demethylases. These results demonstrated that UVA radiation can cause oxidative damage, cell apoptosis, and rat lens opacification by increasing the demethylation of the Keap1 promoter in lens epithelial cells. Conversely, FA was shown to reduce oxidative damage, inhibit cell apoptosis, and decrease rat lens opacification by increasing the methylation of the Keap1 promoter. These findings suggest that FA could be therapeutically beneficial in preventing and mitigating cataracts induced by UVA radiation.

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Influence of cataract light scatters on retinal vessel oxygen saturation

Waizel

Türksever

Todorova

2019

Acta Ophthalmologica

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Background: The aim of our study was to compare metabolic (oxygen saturation; %) and anatomical parameters (retinal vessel diameter; lm) of retinal vessel oximetry (RO) in patients with cataract formation against those of healthy controls with clear lenses. Methods: A total of 96 eyes of 62 subjects were examined: 51 eyes from 33 cataract patients (mean age: 64.8y) were compared to 45 eyes from 29 controls with clear lenses (mean age: 61.5y). RO was performed with the oxygen saturation measurement tool from the RVA (IMEDOS Systems UG). The oxygen saturation in all major peripapillary retinal arterioles (A-SO 2 ) and venules (V-SO 2 ) was measured, and their difference (A-V SO 2 ) was calculated. In addition, the corresponding diameter in retinal arterioles (D-A) and venules (D-V) was evaluated. Cataract formation was graded according to the Lens Opacities Classification System III (LOCS III). Oximetry data were compared with the grade of cataract formation within both groups. For statistical evaluation, ANOVAbased linear mixed-effects models were calculated (SPSS â , pairwise comparisons: Bonferroni-corrected; p < 0.05). Results: Cataract eyes showed significantly lower A-SO 2 and A-V SO 2 values (mean AE SD 92.52 AE 9.80% and 28.56 AE 9.80%), when compared to healthy controls (95.47 AE 4.48% and 34.8 AE 7.08%; p = 0.046 and p = 0.001). Within the cataract group, cortical opacities showed significant interactions with the A-SO 2 , V-SO 2 and the A-V SO 2 parameters (p = 0.027; p = 0.002; and p = 0.026, respectively). Conclusions: These data indicate that the cataract-induced light scatter influences optical retinal oxygen measurements. Cortical opacities showed the highest influence on RO measurement when compared to nuclear opacification, nuclear colour and posterior cataract formation.

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A Novel High Resolution In Vivo Digital Imaging System for the Evaluation of Experimental Cataract in Diabetic Rats

Cited by 13 publications

References 18 publications

Effect of Nifedipine on Severe Experimental Cataract in Diabetic Rats

Effect of Nifedipine on Severe Experimental Cataract in Diabetic Rats

Ferulic Acid Protects Human Lens Epithelial Cells Against UVA‐Induced Oxidative Damage by Downregulating the DNA Demethylation of the Keap1 Promoter

Influence of cataract light scatters on retinal vessel oxygen saturation

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