A novel highly charged exopolysaccharide produced by two strains of Stenotrophomonas maltophilia recovered from patients with cystic fibrosis

Cescutti, Paola; Cuzzi, Bruno; Liut, Gianfranco; Segonds, Christine; Bonaventura, Giovanni Di; Rizzo, Roberto

doi:10.1016/j.carres.2011.05.007

Cited by 19 publications

(8 citation statements)

References 18 publications

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“…We have previously reported that binding of calcofluor white indicates that β-linked polysaccharides, such as cellulose and chitin, are part of the matrix of S. maltophilia biofilms (Passerini de Rossi et al, 2007 ). This is in accordance with the presence of many β-1-4 unions in the primary structure of S. maltophilia exopolysaccharides characterized by Cescutti et al ( 2011 ) from planktonic cultures of two mucoid clinical isolates obtained from two CF patients. This is a novel structure among bacterial polysaccharides: It has three uronic acid residues on a total of four sugars in the repeating unit and bears an additional negative charge due to the d-lactate substituent.…”

Section: Resultssupporting

confidence: 89%

“…The EPS matrix is important in forming the biofilm architecture and in protecting bacteria from antimicrobials and host defense mechanisms. Exopolysaccharides, fundamental components of EPS, are recognized as virulence factors (Cescutti et al, 2011 ; Laverty et al, 2014 ). In order to detect the presence of exopolysaccharides in the matrix of S. maltophilia biofilms samples stained with calcofluor white were examined with epifluorescence microscopy.…”

Section: Resultsmentioning

confidence: 99%

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Iron is a signal for Stenotrophomonas maltophilia biofilm formation, oxidative stress response, OMPs expression, and virulence

et al. 2015

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Stenotrophomonas maltophilia is an emerging nosocomial pathogen. In many bacteria iron availability regulates, through the Fur system, not only iron homeostasis but also virulence. The aim of this work was to assess the role of iron on S. maltophilia biofilm formation, EPS production, oxidative stress response, OMPs regulation, quorum sensing (QS), and virulence. Studies were done on K279a and its isogenic fur mutant F60 cultured in the presence or absence of dipyridyl. This is the first report of spontaneous fur mutants obtained in S. maltophilia. F60 produced higher amounts of biofilms than K279a and CLSM analysis demonstrated improved adherence and biofilm organization. Under iron restricted conditions, K279a produced biofilms with more biomass and enhanced thickness. In addition, F60 produced higher amounts of EPS than K279a but with a similar composition, as revealed by ATR-FTIR spectroscopy. With respect to the oxidative stress response, MnSOD was the only SOD isoenzyme detected in K279a. F60 presented higher SOD activity than the wt strain in planktonic and biofilm cultures, and iron deprivation increased K279a SOD activity. Under iron starvation, SDS-PAGE profile from K279a presented two iron-repressed proteins. Mass spectrometry analysis revealed homology with FepA and another putative TonB-dependent siderophore receptor of K279a. In silico analysis allowed the detection of potential Fur boxes in the respective coding genes. K279a encodes the QS diffusible signal factor (DSF). Under iron restriction K279a produced higher amounts of DSF than under iron rich condition. Finally, F60 was more virulent than K279a in the Galleria mellonella killing assay. These results put in evidence that iron levels regulate, likely through the Fur system, S. maltophilia biofilm formation, oxidative stress response, OMPs expression, DSF production and virulence.

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Iron is a signal for Stenotrophomonas maltophilia biofilm formation, oxidative stress response, OMPs expression, and virulence

et al. 2015

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“…Since the 1 H NMR spectrum indicated the presence of O-acetyl groups (Figure F in S1 File ) which increase the complexity of the spectrum [ 42 ], fraction CIII was de-O-acetylated for further NMR analysis. The 1 H NMR spectrum ( Fig 1A ) is typical of a polysaccharide: the anomeric proton region, between 5.5–4.8 ppm, ( inset of Fig 1A ) exhibited a complex pattern, reflecting the glycosidic linkage data and suggesting the absence of regular repeating units.…”

Section: Resultsmentioning

confidence: 99%

Tramesan, a novel polysaccharide from Trametes versicolor. Structural characterization and biological effects

et al. 2017

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Mushrooms represent a formidable source of bioactive compounds. Some of these may be considered as biological response modifiers; these include compounds with a specific biological function: antibiotics (e.g. plectasin), immune system stimulator (e,g, lentinan), antitumor agents (e.g. krestin, PSK) and hypolipidemic agents (e.g. lovastatin) inter alia. In this study, we focused on the Chinese medicinal mushroom “yun zhi”, Trametes versicolor, traditionally used for (cit.) “replenish essence and qi (vital energy)”. Previous studies indicated the potential activity of extracts from culture filtrate of asexual mycelia of T. versicolor in controlling the growth and secondary metabolism (e.g. mycotoxins) of plant pathogenic fungi. The quest of active principles produced by T. versicolor, allowed us characterising an exo-polysaccharide released in its culture filtrate and naming it Tramesan. Herein we evaluate the biological activity of Tramesan in different organisms: plants, mammals and plant pathogenic fungi. We suggest that the bioactivity of Tramesan relies mostly on its ability to act as pro antioxidant molecule regardless the biological system on which it was applied.

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“…42 Like P. aeruginosa, S. maltophilia is capable of forming a biofilm within airways. 43,44 The prevalence of S. maltophilia in CF is variable between CF centers and appears to be increasing. U.S. CF Registry data reported a prevalence of 4% in 1996 increasing to 14% in 2010; in some centers, this organism can be found in the sputum of up to 25% of patients with CF.…”

Section: Burkholderia Gladiolimentioning

confidence: 99%

The Microbiome and Emerging Pathogens in Cystic Fibrosis and Non–Cystic Fibrosis Bronchiectasis

Green¹,

Jones²

2015

Semin Respir Crit Care Med

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Chronic pulmonary sepsis is the predominant cause of morbidity for patients with cystic fibrosis (CF) and non-CF bronchiectasis. Previously it was thought that respiratory infection in these patients was mostly limited to a very small number of typical pathogens; however, in recent years there have been increasing reports of infection with other emerging potential pathogens including Burkholderia, Stenotrophomonas, Achromobacter, Ralstonia, Pandoraea, nontuberculous mycobacteria, and fungal species. Furthermore, culture-independent methodologies have established that the lungs of patients with CF and non-CF bronchiectasis comprise mixed microbiological communities of aerobic and anaerobic bacteria, fungal and viral species, collectively referred to as the lung microbiome. This article addresses the clinical relevance of emerging pathogens and the lung microbiome in CF and non-CF bronchiectasis.

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A novel highly charged exopolysaccharide produced by two strains of Stenotrophomonas maltophilia recovered from patients with cystic fibrosis

Cited by 19 publications

References 18 publications

Iron is a signal for Stenotrophomonas maltophilia biofilm formation, oxidative stress response, OMPs expression, and virulence

Iron is a signal for Stenotrophomonas maltophilia biofilm formation, oxidative stress response, OMPs expression, and virulence

Tramesan, a novel polysaccharide from Trametes versicolor. Structural characterization and biological effects

The Microbiome and Emerging Pathogens in Cystic Fibrosis and Non–Cystic Fibrosis Bronchiectasis

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