A Novel HPLC Method for the Determination of Alpha-Keto Acids in Human Serum Using Meso Stillbenediamine as Derivatization Reagent

Development of HPLC method for estimation of glyoxylic acid after pre-column fluorescence derivatization approach based on thiazine derivative formation: A new application in healthy and cardiovascular patients’ sera

“…Several analytical methods were reported for determination of GA in different matrices including high performance liquid chromatography (HPLC) [8][9][10][11][12][13][14][15][16][17], gas chromatography (GC) [12,18,19], thin layer chromatography (TLC) [20], electrophoresis [21], voltammetric [22], polarography [20] and electrochemiluminescence [23]. However, most of these methods have several drawbacks either low sensitivity [9-11, 13, 14, 16, 20, 23], utility of expensive and sophisticated equipment that are not available in most laboratories [12,18,19] or long analysis time [11,[15][16][17].…”