A novel human-like collagen hemostatic sponge with uniform morphology, good biodegradability and biocompatibility

Abstract

Biodegradable sponges, as a promising hemostatic biomaterial, has been clinically required over the past decades. Current hemostatic sponges are generally prepared by crosslinking and freeze-drying, but the quality control or biocompatibility is often unsatisfactory due to the freezing-caused morphological non-uniformity and the toxicity of raw materials or cross-linkers. The crosslinking often greatly retards the degradation of the sponges and thus affects the healing of the wound. In this work, we prepared a novel hemostatic sponge using human-like collagen and glutamine transaminase (non-toxic cross-linker) and optimized its morphology via "two-step" freezing instead of conventional "one-step" freezing. The resulting sponge showed a good biocompatibility in cytotoxicity and implantation tests and had a significant hemostatic effect in ear artery and liver injury models. Moreover, the sponge could be degraded high efficiently by several common enzymes in organisms (e.g. I collagenase, trypsase, and lysozyme), which means that the sponge can be easily digested by metabolism and can facilitate seamless healing. Finally, both the front and back of the sponge prepared via two-step freezing was more uniform in morphology than that prepared via one-step freezing. More importantly, two-step freezing can be used as a universal approach for preparation of diverse uniform biomaterials.

Chitosan-Based Composite Materials for Prospective Hemostatic Applications

“…As promising biomaterials for hemostasis, biodegradable sponges have been clinically required in the past decades [72]. GE has been extensively studied as an absorbable hemostatic material for its superior properties such as low antigenicity, good biocompatibility and biodegradability [73,74,75].…”

Biodegradable shape memory polymer foams with appropriate thermal properties for hemostatic applications

“…in vitro enzymatic degradation analysis demonstrated that these microspheres had 40% mass loss in 4 weeks (Li et al, ). Jiang et al () synthesized biodegradable collagen sponges that demonstrated good histocompatibility in vivo, and good hemostatic effect when topically applied on bleeding liver and ear artery. However, the chitosan microspheres and collagen sponges do not have the desirable viscoelastic properties of SMP foams, which led to difficulties in delivery into deep wounds and inadequate pressure on the wounds, indicating that they are not suitable for treatment of noncompressible wounds.…”

“…This low T g range was due to the increased free volume in the amorphous polymer network, resulting from the longer branches of PCL-t compared to the traditionally used monomer polyols, N,N,N 0 ,N 0 -tetrakis(2-hydroxypropyl) ethylenediamine (HPED) and triethanolamine (TEA;Singhal et al, 2014 (Li et al, 2017). Jiang et al (2017) synthesized biodegradable collagen sponges that demonstrated good histocompatibility in vivo, and good hemostatic effect when topically applied on bleeding liver and ear artery. However, the chitosan microspheres and collagen sponges do not have the desirable viscoelastic properties of SMP foams, which led to difficulties in delivery into deep wounds and inadequate pressure on the wounds, indicating that they are not suitable for treatment of noncompressible wounds.…”

Prevention of Recurrences in Dupuytren’s Contracture: Are We in the Right Side?

“…Another possible option would be introducing both drugs using a biodegradable sponge [23]. Although this method has not been specifically tested for CCH, and it will probably be more difficult to adequate such a method to CCH administration, it offers multiple possibilities, acting in the first step as a hemostatic.…”