A novel Aspergillus oryzae esterase that hydrolyzes 4‐hydroxybenzoic acid esters

Abstract

Edited by Miguel De la RosaKeywords: Esterase 4-Hydroxybenzoic acid ester Paraben Aspergillus oryzae a b s t r a c tIn this study we report the biochemical characterization of a hypothetical protein from Aspergillus oryzae exhibiting sequence identity with feruloyl esterase and tannase from the genus Aspergillus. The purified recombinant protein showed a hydrolytic activity toward the ethyl, propyl, or butyl esters of 4-hydroxybenzoic acid, but did not show feruloyl esterase or tannase activity. Finally, the enzyme decreased the antimicrobial activity of parabens against A. oryzae via hydrolysis of the ester bond present in butyl 4-hydroxybenzoic acid.

An enzyme from Auricularia auricula-judae combining both benzoyl and cinnamoyl esterase activity

“…The molecular mass (36 kDa) and pI (3.2) of EstBC was found to be within the typical literature values of fungal FAEs and benzoyl esterases [14,17,25]. The pH optima of FAEs described in literature was between 4.5 and 7.6 [26].…”

“…The pH optima of FAEs described in literature was between 4.5 and 7.6 [26]. However, the pH optimum of benzoyl esterases was found to be at higher pH values ranging from 7.0 to 9.0 [14,17,25]. The pH optimum of EstBC at 6.5 corresponded most to the literature values of FAEs.…”

“…These ranged from 37 to 60 °C [26]. The thermal stability properties of EstBC were with 50% residual activity at 65 °C greater than other FAEs and benzoyl esterases [14,17,25,26]. high k cat values for CA-ME [27][28][29].…”

“…There is no consistent nomenclature for benzoyl esterases. They are reported as enzymes hydrolyzing benzoyl esters of simple sugars [12], as lipases acting on oleyl benzoate [13] and esterases cleaving 4-hydroxybenzoic acid esters [14]. Applications of benzoyl esterases are the enantioselective synthesis of flavors, fine chemicals and pharmaceuticals [4,15].…”

A Novel Alkaline Esterase fromSporosarcinasp. nov. Strain eSP04 Catalyzing the Hydrolysis of a Wide Variety of Aryl-carboxylic Acid Esters

“…Enzymes hydrolyzing 4-hydroxybenzoic acid esters are considered to belong to a different esterase family from benzoyl esterases. 17,18) Aryl-carboxylesterases, however, have many potential applications in various fields. They are attractive biocatalysts for the production of various aromas, fragrances, 19,20) and preservatives, 17,18) for the selective removal of protecting groups, 21,22) and in the field of health [23][24][25][26] as well as being potentially beneficial for the environment.…”