A novel IFNbeta-induced long non-coding RNA ZAP-IT1 interrupts Zika virus replication in A549 cells

Huang, Yanxia; Su, Yu‐Ping; Shen, Li; Huo, Zhiting; Chen, Cancan; Sun, Tao; Xu, Tongwen; Li, Ning; Yang, Chia-Han

doi:10.1016/j.virs.2022.08.003

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…25 Many studies have reported that ISGs play critical roles to restrict ZIKV replication. [27][28][29][30][31][32][33] For example, Viperin has been reported…”

Section: Discussionmentioning

confidence: 99%

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UMP‐CMP kinase 2 inhibits ZIKV replication through activation of type I IFN signaling pathway

Zhu,

Tan,

Shi

et al. 2024

Journal of Medical Virology

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Cytidine/uridine monophosphate kinase 2 (UMP‐CMP kinase 2, CMPK2) has been reported as an antiviral interferon‐stimulated gene (ISG). We previously observed that the expression of CMPK2 was significantly upregulated after Zika Virus (ZIKV) infection in A549 cells. However, the association and the underlying mechanisms between CMPK2 induction and ZIKV replication remain to be determined. We investigated the induction of CMPK2 during ZIKV infection and the effect of CMPK2 on ZIKV replication in A549, U251, Vero, IFNAR‐deficient U5A and its parental 2fTGH cells, Huh7 and its RIG‐I‐deficient derivatives Huh7.5.1 cells. The activation status of Jak‐STAT signaling pathway was determined by detecting the phosphorylation level of STAT1, the activity of interferon stimulated response element (ISRE) and the expression of several interferon stimulated genes (ISGs). We found that ZIKV infection induced CMPK2 expression through an IFNAR and RIG‐I dependent manner. Overexpression of CMPK2 inhibited while CMPK2 knockdown promoted ZIKV replication in A549 and U251 cells. Mechanically, we found that CMPK2 overexpression increased IFNβ expression and activated Jak/STAT signaling pathway as shown by the increased level of p‐STAT1, enhanced activity of ISRE, and the upregulated expression of downstream ISGs. These findings suggest that ZIKV infection induced CMPK2 expression, which inhibited ZIKV replication and serves as a positive feedback regulator for IFN‐Jak/STAT pathway.

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“…25 Many studies have reported that ISGs play critical roles to restrict ZIKV replication. [27][28][29][30][31][32][33] For example, Viperin has been reported…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have reported that ISGs play critical roles to restrict ZIKV replication 27–33 . For example, Viperin has been reported to inhibit ZIKV RNA translation by activating ribosome collision‐dependent pathway 27 .…”

Section: Discussionmentioning

confidence: 99%

UMP‐CMP kinase 2 inhibits ZIKV replication through activation of type I IFN signaling pathway

Zhu,

Tan,

Shi

et al. 2024

Journal of Medical Virology

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“…LncRNA-SUSAJ1 , in turn hampers JEV replication and interrupts the transmission of JEV [ 115 , 116 ]. In addition, several broad-spectrum regulatory lncRNAs, such as JINR1 , ZAP-IT1 , MALAT1 , and Gm20559 , are characterized in flaviviral diseases [ 117 , 118 , 119 , 120 ]. For instance, the flavivirus-induced lncRNA JINR1 , mediated by NF-κB, is considered to be the facilitator of EV/DENV/WNV replication.…”

Section: Lncrnas In Viral Zoonosesmentioning

confidence: 99%

“…It functions by interacting with RNA-binding protein RBM10, and manipulating the expression of NF-κB target genes, such as glucose-regulated protein 78 ( GRP78 ) [ 117 ]. Another lncRNA ZAP-IT1 is however induced by type I IFN, and is recognized as a negative regulator for flavivirus infection, and as inhibiting the replication of ZIKV, DENV, JEV and vesicular stomatitis virus (VSV) in a type I IFN signaling independent manner [ 118 ]. Interestingly, from the perspective of pathogens, all members of the Flaviviridae are likely to produce lncRNAs in their infected cells, these lncRNAs are generated by the stalling and degrading of host exonuclease Xrn1 on viral RNA structures, which impacts viral replication, cytopathology as well as pathogenesis, opening up the door to new therapeutic targets for the development of broad-spectrum antiflaviviral therapeutics [ 121 ].…”

Section: Lncrnas In Viral Zoonosesmentioning

confidence: 99%

Unveiling the Hidden Regulators: The Impact of lncRNAs on Zoonoses

Xu,

He,

Yang

et al. 2024

IJMS

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Zoonoses are diseases and infections naturally transmitted between humans and vertebrate animals. They form the dominant group of diseases among emerging infectious diseases and represent critical threats to global health security. This dilemma is largely attributed to our insufficient knowledge of the pathogenesis regarding zoonotic spillover. Long non-coding RNAs (lncRNAs) are transcripts with limited coding capacity. Recent technological advancements have enabled the identification of numerous lncRNAs in humans, animals, and even pathogens. An increasing body of literature suggests that lncRNAs function as key regulators in zoonotic infection. They regulate immune-related epigenetic, transcriptional, and post-transcriptional events across a broad range of organisms. In this review, we discuss the recent research progress on the roles of lncRNAs in zoonoses. We address the classification and regulatory mechanisms of lncRNAs in the interaction between host and zoonotic pathogens. Additionally, we explore the surprising function of pathogen-derived lncRNAs in mediating the pathogenicity and life cycle of zoonotic bacteria, viruses, and parasites. Understanding how these lncRNAs influence the zoonotic pathogenesis will provide important therapeutic insights to the prevention and control of zoonoses.

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Bibliometric analysis of flavivirus-host interactions and immune responses: Research hotspots and trends

Xiong,

Fan,

2024

Biosafety and Health

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A novel IFNbeta-induced long non-coding RNA ZAP-IT1 interrupts Zika virus replication in A549 cells

Cited by 6 publications

References 47 publications

UMP‐CMP kinase 2 inhibits ZIKV replication through activation of type I IFN signaling pathway

UMP‐CMP kinase 2 inhibits ZIKV replication through activation of type I IFN signaling pathway

Unveiling the Hidden Regulators: The Impact of lncRNAs on Zoonoses

Bibliometric analysis of flavivirus-host interactions and immune responses: Research hotspots and trends

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