A Novel Image Probing System for Precise Quantification of Angiogenesis

Ejaz, Sohail; Seok, Kim Bum; Woong, Lim Chae

doi:10.1177/030089160409000614

Cited by 18 publications

(12 citation statements)

References 56 publications

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“…The CAM assay is rapid, technically simple and inexpensive [85], [86], [87]. To observe the development of embryo, Chick chorioallantoic membrane assay model is very helpful to precede research as it is impossible in other systems like mammalian system [88].The development of floating chick embryo and the changes which occur in nutritious egg yolk are easy to manipulate with the help of CAM model (Figure 8).…”

Section: Chicken Chorio-alntoic Membrane (Cam) Assaymentioning

confidence: 99%

Assessment Methods of Angiogenesis and Present Approaches for Its Quantification

Khan¹,

Shakir²,

Khan³

et al. 2014

CRJ

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Angiogenesis is the extension of blood vessels from the prevailing vessels. It is an essential process in the formation and development of blood vessels, so it is supportive in healing of wound while its inhibition may help to restrict the size of tumor. To quantify the effect of an agent on angiogenesis, a standardized preclinical screening assay is necessary. The objective of this review is to document all the methods of angiogenesis assessment and specific techniques to quantify the results, majorly focusing on the modes of calculation and concerns that researchers need to make before reaching to any conclusion. Scientific research on angiogenesis relies on different models for assessment, including in vitro, in vivo and in ovo models. Many of these assays are practiced to test the effect of both pro and anti angiogenic agents. The findings articulated in this study will help to provide better visions into the pathophysiology of diseases associated with angiogenesis and to establish new as well as appropriate angiogenic or anti angiogenic treatment strategies for the future. Nevertheless, angiogenesis is a vigorous process and the techniques to evaluate this process with thorough assessment of physiological characteristics of new blood vessels will be advantageous over the older systems of angiogenesis assessment methods.

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Section: Chicken Chorio-alntoic Membrane (Cam) Assaymentioning

confidence: 99%

Assessment Methods of Angiogenesis and Present Approaches for Its Quantification

Khan¹,

Shakir²,

Khan³

et al. 2014

CRJ

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“…Same procedure was adopted as reported in our previous publications [21][22][23]. Briefly, White Leghorn chicken (Gallus domesticus) eggs were incubated at 37°C (humidity 55-60%) after sterilization.…”

Section: Preparation Of Chicken Chorioallantoic Membranementioning

confidence: 99%

A novel link between angiogenesis and natural products: Anti-angiogenic effects of Opuntia dillenii

et al. 2013

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Angiogenesis is the formation of new blood vessels from the existing blood vessels and is involved in both physiological and pathological events. Pathological angiogenesis provokes in several important diseases as in inflammatory diseases, ischemic conditions, diabetic neuropathy and cancer. The discovery of new drugs from phyto-chemicals has a long history and anti-angiogenic agents from plant sources provide a platform for the development of phyto-medicines to treat/control pathological angiogenesis. The current project was designed to determine the efficacy of different concentrations of aqueous extract from Opuntia dillenii (OD) on angiogenesis by employing chicken chorioallantoic membrane (CAM) assay. Computer based 3D image probing was utilized to quantify anti-angiogenic effects of OD extract. Extremely aggravated dose dependent anti-angiogenic response was recorded in all groups treated with OD extract with significant reduction (P<0.01) in total area, diameter of the primary, secondary, tertiary blood vessels and capillary plexuses. Analysis of 3D parameters of OD treated CAMs revealed deteriorated angular spectrum and reduction in the height of Abbott curves. In addition, histological data revealed thinning of ectodermal layer and damaged extracellular matrix. The results spotlight that OD extract possess considerable anti-angiogenic potential and further characterization/isolation of diverse phyto-chemicals would turn up with the discovery of novel phyto-agents to control/mitigate pathological angiogenesis.

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“…A great deal of information regarding IPS (a combination of SPIP software and Adobe Photoshop 6.0) already has been explained in our previous study (13). The display screen (IBM-LG, Korea) supported with SVGA graphics (NVIDIA GeForce 2 MX 100/200) and adjusted to resolution 800 × 600 pixels, provided optimal optical representation.…”

Section: Image Processing Systemmentioning

confidence: 99%

“…Sq is a dispersion parameter defined as the root mean square value of the surface within the sampling area. Sq is a very general and widely used parameter for evaluating roughness profile ordinates (13). Sz is an extreme parameter used to evaluate the average value of the absolute heights of the 5 highest peaks within the sampling area.…”

Section: Image Processing Systemmentioning

confidence: 99%

“…There are several reasons for this tendency as they are easy to measure; literature and standards are available to explain these parameters. All these parameters provide detailed information about micrometer and/or nanometer scale neovascularization on bearing surfaces (13). More specifically, micrometer and/or nanometer scale topographies represent different aspects of cell behavior such as cell adhesion, cell proliferation, cell differentiation, cell orientation, production of local factors, and tissue organization, which are important factors of angiogenesis (14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

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A Novel 3-D Model of Chick Chorioallantoic Membrane for Ameliorated Studies in Angiogenesis

Ejaz

Chekarova

Ashraf

et al. 2006

Cancer Investigation

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Decisive indulgence of angiogenesis requires a more holistic assessment and several in vivo assays have been developed that permit a more realistic appraisal of the angiogenic response. One of the most popular assays to study angiogenic activity is the chick chorioallantoic membrane (CAM). Although CAM assay is a vital technique used to study normal and putative angiogenesis, a serious drawback in its utilization is the lack of quantitative assessment of vascularization. In this study, we proposed a new 3-D model of the developing CAM for precise quantification of normal vasculature of CAM from Day 4 to Day 13 of incubation. Image probing technique was used to quantify different 3-D parameters of vascular microarchitecture. A significant increase (P < 0.05) in surface roughness (Sa) was observed at Day 5 of incubation, while highly significant increase (P < 0.01) in Sa values was observed at Day 6 of incubation. Maximum increase (P < 0.001) in Sa values was observed from Day 7 to Day 9 of incubation and slight decrease in Sa values was observed in successive days. Similar results were observed for root mean square values (Sq), absolute heights of the surface (Sz), and amount of lowest valleys (Sy) on CAM. A significant increase (P < 0.05) in developed surface area (Sdr) from Day 5 of incubation, reaching to its maximum (P < 0.01) at Day 9 also was noted. Similarly, significant increase (P < 0.05) in fluid core retention (Sci) illustrates presence of copious fluid in blood vessels. Image probing technique offers a useful modality for visualizing 3-D microvascular architecture of CAM to exaggerate the fine details and reveal the hidden information that can be helpful for precise quantification of angiogenesis. This approach can be used to evaluate the angiogenic and antiangiogenic potential of different biological substances and also can be a valuable independent prognostic indicator in a wide variety of human cancers.

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A Novel Image Probing System for Precise Quantification of Angiogenesis

Cited by 18 publications

References 56 publications

Assessment Methods of Angiogenesis and Present Approaches for Its Quantification

Assessment Methods of Angiogenesis and Present Approaches for Its Quantification

A novel link between angiogenesis and natural products: Anti-angiogenic effects of Opuntia dillenii

A Novel 3-D Model of Chick Chorioallantoic Membrane for Ameliorated Studies in Angiogenesis

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